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8/31/2020
Stage automation
Caution: whenever the Tile Scan or Mark and Find window is launched
i. any tiles or points marked will be processed when you click Start
ii. the grey area represents the stage, a left mouse click will cause the stage to move to that specific
location and can be dangerous for any objective lenses except the 10×
Tile Scan
1. click
2. check that Scan Field Rotation is set correctly (see
3. go Live
4. move the prep/stage to the left boundary of the region of interest, focus
5. click
6. repeat the last 2 steps for the top, right, and bottom boundries, the software will calculate the matrix
needed
Note: Marking any points outside the established coverage area will expand the matrix but
marking any points inside will not change anything. Unfortunately, there is no provision to
save the points here or apply any points already saved from Mark and Find.
7. move about to make sure the coverage is sufficent, especially with multichannel acquistion
8. setup Z-stack, configure Average etc., as needed
9. click Start to acquire data
Mark and Find
1. click
2. if there are existing coordinates, you can save or clear them first
3. go Live
4. move the prep/stage to the desirable area and focus on the object of interest
5. click
6. repeat the above 2 steps to mark more positions as needed, i.e., positionN+1, N+2... etc.
7. you can go back to any position by selecting it from the drop down list
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
to toggle displaying the Tile Scan window
to mark the current position
to toggle displaying the Mark and Find window
to mark the current position, which will be listed as positionN
below)
r e l e v a n t d e t a i l s
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