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8/31/2020
Z
software control of the z-drive
This provides alternate means to control the z-Position only when
the Smart Panel is unavailable
enter a number directly into the text boxes of z-Position,
Begin, or End
mouse: place cursor on z-plane (cursor change into double
arrow), either click and drag, or click and then use thumb
wheel
Caution: Use extreme caution with mouse control! It is
very easy to drag the z-position too far and ram the
objective onto the slide and damage the objective.
setting z-step size or slice thickness
the general guideline is to set the z-step size to ½ to ⅓ of
the section thickness (clickable link) shown in the XY panel
section thickness (dz) is dependent on the emission
wavelength (λ), refractive index of the immersion
medium (n) and NA of the objective, and the size of
the pinhole (AU)
dz = √((λ×n÷NA²)²+(AU×n×√2×1.22×λ÷NA²)²)
section thickness can be adjusted to the mean of the
emission wavelength by following the link and then
Apply to the step size
usually the shortest wavelength emission band is used
to set the section thickness in a multichannel scan
use System optimized in the Z-Stack panel if you plan to do
deconvolution
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
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