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Leica SP5 II Instructions Manual page 11

Laser scanning confocal microscope
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8/31/2020
AP: aperture diaphragm for TL
INT : light intensity, press both together to toggle between Coarse and Fine mode
FD: field diaphragm for IL
3. front
top row switches should not be activated, or
else it may block viewing through the
binocular, to rectify
SHUTTER: for epifluorescent illumination
bottom 6 buttons: for selecting specific cube
(DAPI, GFP, DsRed) for epifluorescence; press
the middle 2 buttons together to display the programs assigned to the variable function buttons
4. right rear variable function buttons
CHGOBJ ↻: rotates the objective turret one position
clockwise in the current mode (dry or immersion)
CHGOBJ ↺: same as above but counterclockwise
DRY/IMM: toggles between dry and immersion mode
5. right front: Focus buttons
Z ↑: moves the Z drive up when a upper focus stop has
been set
Z ↓: moves the Z drive down
SET + Z ↑: toggles set and unset upper focus stop
SET + Z ↓: toggles set and unset lower focus stop
SmartMove remote control for stage movements, as observed in the viewer
display window of the software
1. X
2. Y
3. Z (focus, z-wide)
4. left button set selects Precise or Fast XY movement
5. right button set selects FINE or COARSE focus control
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
press the leftmost button to select
binocular
press the rightmost button to deselect
the supplemental magnification changer
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