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8/31/2020
relevant details
for tile scan, current rotation calibration for stitching is -0.19 ° (-0.18 ° is also OK) with a 10% overlap
tile scan order is row-wise in the viewer display window, starting from the lower left, which
corresponds to column-wise starting from the lower right as displayed in the Tile Scan window
Note: The orientation of the specimen as shown in the viewer display window (1) is rotated
when compared to that as observed through the ocular of the microscope (2) or represented
in the Tile Scan or Mark and Find window (3). Confusing? Indeed!
1. viewer display window
3. Tile Scan or Mark and Find window
viewer display windows rotated 90 ° left
coordinate system
values (mm) displayed in the window denotes the upper right corner of the image in the viewer
display window
upper left of the stage is 0, 0
lower right of the stage is 126, 82
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
2. at the microscope
viewer display windows rotated 90 ° right
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