Advertisement
8/31/2020
Set up acquistion parameters and acquire an image
1. Beam Path Settings in the central Work area: there are 3 ways to
configure excitation and emission parameters
A. use a preset
i. click on Load/Save single setting
ii. select a Leica or User Settings (for multichannels, the default
is simultaneous scan, see
B. reuse a setting from a previous scan
i. open a previous experiment
ii. in the Experiments tab, click to select the desirable scan
iii. right-click to choose Apply Setting or hit Apply button at the bottom
C. assemble from scratch
i. set laser power
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
below for sequential
activate the shutter
set power level of the desirable laser
line, 5 % is a good starting point
scan)
2/16
Advertisement
