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Leica SP5 II Instructions Manual page 9

Laser scanning confocal microscope
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8/31/2020
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1. lower the turret, remove your prep; if applicable, remove oil/immersion fluid on immersion objectives
2. switch to the 10× objective
3. if there are users signed up within the next 6 hours do the following, otherwise go to the next step
i. save your experiments and quit LAS AF
ii. copy your data to external storage media or network file servers, as needed
iii. submit your usage using the google form and log out of Windows
4. if no one signed up within the next 6 hours, please proceed to shutdown everything
i. save your experiments and quit LAS AF
ii. Main Switch Board: turn the key counterclockwise to Off-0 to disable Laser Emissions
iii. Main Switch Board: wait 10 s, switch off Scanner Power
iv. copy your data to external storage media or network file servers, as needed
v. submit your usage using the google form
vi. shutdown Windows XP
vii. Main Switch Board: after computer has shutdown, switch off PC Microscope
viii. Main Switch Board: when the Argon laser cooling fan stops, switch off Laser Power
ix. turn off FLUO, if applicable
x. put the cover back on the microscope
5. clean up the work area
file:///E:/pang/work/web-oringinal/sp5_inst_print.shtml
Instruction for Leica SP5 confocal microscope
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