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Mix. The [Mix] key programs the pipette to perform a combination of fills
and dispenses. You can program a mix volume or mix cycle (single fill and
dispense). You can press [Mix] once to program a mix volume, or twice to
program a mix cycle. Mix cycles from 2 to 9 are available on the pipette.
The default setting is three mix cycles.
Some programming considerations for the mix operation:
• If the program begins a mix cycle from zero position, it will complete the
cycle with an automatic blowout, then display "ZERO." Press the trigger
to return the pistons to zero position.
• If the program begins a mix cycle above zero position (volume is
greater than zero), the fluid is dispensed, and the mix cycle begins.
The mix cycle will end at the same volume it started with. Also, the mix
operation will remain in the display so it can be repeated (useful for
serial dilutions), unless followed by another program step. To end the mix
operation, press the [Purge] key, then press the trigger.
Purge. The [Purge] key programs the pipette to purge the fluid. This step can
be entered as a program step, or it can be pressed any time while pipetting to
terminate the program and dispense the remaining liquid.
Note: If the program ends at a volume greater than zero, and the last step is
not a mix cycle, the "Purge" prompt automatically appears. Also, the pipette
emits a double beep tone to warn you that some fluid remains. Press the trigger
to dispense the remaining fluid and return to the beginning of the program.
Examples of pipetting programs with different mix steps are shown in
Section C.2, Programming Examples.
3. Enter volume. Enter the desired volume for [Fill], [Disp], or [Mix] by
pressing the up s or down t section of the [Vol] key. The longer you hold
the key, the greater the incremental speed.
Note: The [Purge] key does not use a volume; therefore, if a purge step has
been entered, continue to the next step.
Programming and Operation
15
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