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Programming and Operation
2. Select pipetting operation. Enter the programming mode by pressing the
[Fill] or [Mix] key.
For example, to begin
programming with a filling
operation, press the [Fill] key.
The display shows "Fill" with
a flashing "Vol" prompt:
Note: The displayed volume defaults to the last volume programmed for the
selected operation, or to the default volume for the pipette, if it is being programmed
for the first time.
Fill. The [Fill] key programs the pipettor to aspirate a volume of liquid. A
single fill step can be programmed for fluid transfers, or a combination of fill
steps can be programmed for dilutions or supernatant collection.
Dispense. The [Disp] key programs the pipette to dispense a volume of liquid.
The dispense volume can either be the entire fill volume or a smaller increment
for repeat pipetting. The dispense volume needs to be programmed only once
for repeat pipetting. The pipette will dispense the volume each time the trigger
is pressed (or repeatedly during a paced dispense) until the fill volume is
reached, or until insufficient volume remains.
14
Fill
Volume
Volume
12.50
10.00
30.0
25.0
125.0
100.0
250.0
250.0
850
850
1250
1250
Table C.1.2: Default Programming Volumes (in µl)
Dispense
Volume
Volume
10.00
5.00
25.0
10.00
100.0
50.0
50.0
50.0
100
100
Figure C.1.2a: Flashing Volume Display
Mix
100
100
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