Trial Run; Cold Room Installation - Beckman Coulter Airfuge Instructions For Use Manual

Installation
Installation Procedures

Trial Run

When installation is complete, perform a trial run to confirm proper ultracentrifuge operation.
Refer to CHAPTER 3
1
After the rotor runs for a few minutes, turn the
The run light should go out approximately 2 minutes after the
•
2
Observe rotor motion through the window.
• Fixed angle rotors should stop within about 2 minutes after the run light goes out.
• Other rotors should stop within about 3½ minutes.
NOTE
If a fixed angle rotor stops in less than 45 seconds, sample stirring may result; adjust the brake
or levitation air to provide a longer stopping time. If a rotor takes too long to stop, the brake, pin
tension, or levitation air needs adjusting. See

Cold Room Installation

If rotor temperatures below room temperature are required, the Airfuge can be operated in a cold
room. A typical cold room set-up is shown in
above, the following variations are required.
1
Because large amounts of water may condense in the airstream as it passes through the lower
temperatures of the cold room, a second filter is required to remove the additional water.
Install the second filter as close as possible to the ultracentrifuge.
The maximum distance between the filter and the instrument should be 0.6 m (2 ft).
2
The second filter will cause a pressure drop of about 5 psig (35 kPa) in the air pressure that
reaches the instrument. Make sure that the required operating air pressure is obtainable at the
instrument pressure gauge.
2-6
for operating instructions. Time the rotor deceleration as follows.
dial to zero and begin timing.
TIME
CHAPTER 5 for adjustment procedures.
Figure 2.3. In addition to the installation procedures
E dial is turned off.
TIM
AF-IM-13AC