Thermo Scientific VWD-3100 Operating Instructions Manual

Thermo Scientific VWD-3100 Operating Instructions Manual

Variable wavelength detectors
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Thermo Scientific Dionex
UltiMate 3000 Series
Variable Wavelength Detectors
VWD-3100 and VWD-3400RS
Operating Instructions
(Original Operating Instructions)
Revision: 1.7
Date: September 2013
© 2013 Thermo Fisher Scientific Inc.
Doc No. 4820.7451

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Summarization of Contents

Introduction and Safety
How to Use this Manual
Explains the manual's layout and conventions for user understanding.
Safety Precautions
Details symbols and critical safety guidelines for instrument and chemical handling.
Intended Use
Defines the device's purpose and scope of use.
FCC Compliance Note
FCC compliance information for the device.
Detector Overview and Components
Unit Description
Provides a detailed description of the detector's features and capabilities.
Operating Principle
Explains how the detector works based on photometric detection and Lambert-Beer's Law.
Detector Configurations
Lists available detector configurations and their part numbers.
Interior and Front Panel Elements
Describes internal parts and front panel elements for operation and monitoring.
Detector Connections and Interfaces
Front Panel Elements
Details the components and indicators on the detector's front panel.
Rear Panel Ports
Identifies and describes ports and connectors on the rear panel.
Fluid Connections
Explains how to route capillaries for fluid connections.
Flow Cells and Lamps
Details available flow cells and detector lamps for optimal performance.
Installation and Setup
Facility Requirements
Specifies site requirements for instrument installation.
Unpacking and Positioning
Provides instructions for unpacking and positioning the detector in the system.
Detector Connections
Step-by-step guide for connecting the detector's cables and ports.
Chromeleon Setup
Instructions for setting up the detector within the Chromeleon software.
System Preparation and Equilibration
Startup Overview
Outlines the key actions required to prepare the detector for initial operation.
Capillary Connection Precautions
Details general precautions for safely connecting capillaries.
Drain Tubing Connection
Instructions for connecting the drain tubing for waste discharge.
System Equilibration
Steps for equilibrating the entire system before sample analysis.
Detector Operation
Power-Up and Status Screens
Describes the detector power-on sequence and status screen information.
Detector Control Methods
Details control via front panel display or Chromeleon software.
Display Screens and Menus
Explains how to use function keys and menus on the detector display.
Operating Information
Provides general notes and information for operating the detector.
Detector Settings and Data Acquisition
Setting Wavelengths
Instructions for setting operating wavelengths in Chromeleon or on the display.
Lamp Control
Details on turning lamps on automatically or manually for operation.
Data Acquisition Control
Procedures for starting and stopping data acquisition.
Advanced Operation and Monitoring
Display Mode and Leak Detection
How to set display modes and manage leak detection settings.
Temperature and Screen Settings
How to record temperature signals and adjust screen brightness/contrast.
Startup and Shutdown Automation
Information on automating startup and shutdown procedures.
Performance Optimization
Performance Optimization Parameters
Discusses selecting parameters to optimize detector performance.
Baseline Behavior
How to define baseline behavior during wavelength switching.
Recording Absorbance Spectra
Procedures for recording absorbance spectra for analysis.
Special Functions and Diagnostics
Predictive Performance
Functions for estimating consumable lifetime and monitoring service.
Detector Diagnostics
Using Chromeleon for diagnostic tests to check detector performance.
Qualification Procedures
Procedures for checking and documenting HPLC system performance.
Detector Shutdown and Maintenance
Detector Shutdown
Precautions and procedures for shutting down the detector.
Routine Maintenance
Lists maintenance procedures for ensuring optimal performance.
Troubleshooting
Troubleshooting Overview
Introduces features for identifying and resolving operational problems.
Detector Display Messages
Lists detector-related messages and recommended remedial actions.
Chromeleon Diagnostics Messages
Remedial actions for detector failures during Chromeleon diagnostics tests.
Operating Problems
Common Operating Problems
Lists common operating problems, probable causes, and remedial actions.
Service Procedures
General Safety Precautions
Describes precautions for user-performed maintenance and safety guidelines.
Wavelength Calibration
Explains automatic wavelength calibration and manual verification procedures.
Lamp Maintenance
Safety precautions and instructions for replacing deuterium and tungsten lamps.
Flow Cell Maintenance
Information on dummy flow cells, cleaning, and replacing flow cells.
Detector Component Maintenance
Drying the Leak Sensor
Steps to dry the leak sensor after a leak is detected.
Replacing Power Fuses
Instructions for replacing the main power fuses.
Updating Detector Firmware
Procedures for updating the detector's firmware version.
Technical Information
Technical Specifications
Lists detailed specifications including optical design, light sources, range, noise, linearity.
Accessories and Parts
Standard Accessories
Lists standard accessories shipped with the detector.
Optional Accessories
Lists optional accessories available for the detector.
Consumables and Spare Parts
Lists consumables and spare parts for the detector.
Appendix Information
Common Mobile Phases
Provides information on properties of frequently used mobile phases.
Digital I/O Ports
Details the pin assignment and functions of the digital I/O ports.
Declaration of Conformity
Declaration of conformity for holmium oxide filters used for wavelength verification.

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