Agena BIOSCIENCE MassARRAY Nanodispenser RS1000 User Manual page 154

Appendix D Troubleshooting
152
Issue
Dispensed volume too
low.
Dispensed volume too
variable.
Incorrect number of
fiducials (i.e., incorrect
SpectroCHIP Array
alignment)
Unable to read plate
barcode.
Unable to read
SpectroCHIP Array
barcode.
Notification Location
During a run: volumes are shown in
the Volume tab of the Transfer
screen.
After a run: A warning message
appears if any dispenses were out
of range.
Transfer tab will show a CV(%) that is
too high.
Warning message appears at start
of dispensing run.
Warning message appears at start
of dispensing run.
Warning message appears at start
of dispensing run.
MassARRAY® Nanodispenser RS1000 v2.1 User Guide
For Research Use Only. Not for use in diagnostic procedures.
Recommended Action
Increase dispense speed.
At the desalting step, use a 0.001%
Tween and HPLC-grade water
solution in place of the regular water
addition.
For UltraSEEK panels only: re-
dispense analyte from the reaction
plate onto the SpectroCHIP Array,
i.e., double dispense the analyte
onto the same pads. This increases
the number of analyte molecules,
thereby increasing signal intensity.
Use the final dispensed volume
from the first dispensing run as the
initial volume for the second
dispensing run.
Increase aspirate time and/or
dispense time.
At the desalting step, use a 0.001%
Tween and HPLC-grade water
solution in place of the regular water
addition.
Ensure that the SpectroCHIP Array
is properly seated on the deck.
If that does not solve the problem,
retry with a new SpectroCHIP Array.
Ensure that the recommended plate
type is being used.
Attempt to restart dispensing run.
If that does not solve the problem,
retry with a new plate with a new
barcode.
Attempt to restart dispensing run.
If that does not solve the problem,
retry with a new SpectroCHIP Array.
See
To set the dispensing
speed:, page 37
Desalting the Analyte,
page 21
Dispensing to
SpectroCHIP Arrays,
page 19
and
Accessing dispense
volume data from
previous runs, page 56
Recommended
Dispense Settings,
page 31
Selecting aspirate/
dispense parameters,
page 37
Loading the
SpectroCHIP Arrays,
page 45
Removing the
SpectroCHIP Arrays,
page 54
Table 1.8
Tap the run button on the
Transfer screen.
Setting up the
dispensing run, page 30
Tap the run button on the
Transfer screen.
Setting up the
dispensing run, page 30
USG-CUS-059 Rev05