How Much Sample Do I Need; An Alternative Way To Fill The Loop - Agilent Technologies G1323B User Manual

How Much Sample Do I Need?

To completely fill the sample loop an excess of sample is required. This is
about 2 to 3 loop volumes of sample to achieve 95 % of the maximum loop
volume (the remainder is residual mobile phase in the sample loop).
Determine the optimum number of loop volumes experimentally for your
particular application.
When you partially fill the sample loop, do not inject more than half of the
sample volume. As you inject your sample into the loop, the sample mixes with
the solvent already in the loop and some of the sample could be lost through
port 6 if you try to inject too much.

An Alternative Way to Fill The Loop

When the sample loop is completely filled you can either inject the sample into
the loop or use the syringe to draw the sample through the loop.
1 Move valve to LOAD position.
2 Place vent tube from port 6 into sample vial.
3 Insert needle of syringe into needle port until needle touches stator face.
4 Slowly draw sample into syringe.
5 Leave syringe in position and move valve to INJECT position.
6 Remove syringe.
If you use this method, always flush the loading passages (vent tube on port 6)
after each injection to prevent cross-contamination between injections.
Remember — to flush port 6 and the vent tube, the valve must be in the LOAD
position.
1200 Series Handheld Control Module Users's Guide
Do not press too hard.
Using the Manual Injection Valve

How Much Sample Do I Need?

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