Preparing The Lc System - Agilent Technologies G1323B User Manual

Preparing the LC System

1 Fill one solvent bottle with bidistilled water (channel A) and the other with
2 Turn on the detector lamp and pump using the On/Off button [F7] in the
3 For the quaternary pump, turn on the degasser by pressing the line-power
4 Purge the pump. For more information see
5 Allow the detector at least 15 minutes to provide a stable baseline.
6 Fill the contents of a Agilent Technologies isocratic standard sample
7 Pump the water/acetonitrile (35/65 %) mobile phase through the column for
1200 Series Handheld Control Module Users's Guide
acetonitrile (channel B).
Analysis screen.
switch.
ampoule into a vial and seal the vial with a cap. Place the vial in position 1
of the autosampler tray.
10 minutes at a flow rate of 2 ml/min.
Running a Gradient Analysis

Preparing the LC System

Chapter 3, "Using the Pump".
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