Adjusting Stroke Volume; Automatic Shut-Down; Troubleshooting The Pump - Agilent Technologies G1323B User Manual

3 Using the Pump

Adjusting Stroke Volume

Adjusting Stroke Volume

The stroke volume defines the volume of mobile phase which is displaced by
one stroke of pump piston 1. You can set the stroke volume to AUTO
(automatic) or a value between 20 and 100 µl. When the stroke is set to AUTO,
the pump uses large strokes at high flow and shorter strokes at low flow rate.
This can improve the mixing performance and gradient linearity by reducing
the size of the solvent packets to be mixed.
The stroke volume can be accessed by using the Settings button [F1] in the
Analysis screen, selecting the pump and then pressing More ...
The amplitude of the pressure pulsation is directly proportional to the stroke
volume. Decreasing the stroke volume decreases the pulsation amplitude,
giving a better signal-to-noise ratio with flow-sensitive detectors. To maintain
the flow, the pumping frequency is increased, increasing the frequency of the
pressure pulsation. Increasing the pump frequency gives better peak area
reproducibility at low flow rates.

Automatic Shut-down

At the end of a sequence you can set the pump to be automatically turned off
using the Sequence End Actions button [F1] in the Sequence screen. You can
turn off the pump completely or load a specific shut-down method.

Troubleshooting the Pump

1 Select the Tests button [F3] in the System screen.
2 Select Pump from the menu.
You can now select various tests to check the pump. For further information
about these tests see the Reference Manual for the Agilent 1200 Series pump.
68 1200 Series Handheld Control Module Users's Guide