Hach BioTector B7000 User Manual page 71

Connect Deionised Water to the BioTector's ZERO WATER port using ¼" PFA tube. The
BioTector TOC TN analyzers require Zero Water to adjust the zero offset during the zero
calibration cycle. This is different than the BioTector TOC analyzers, where only acid and
base reagents are required. The reason is that the TN measurements are made in the liquid
phase, so the makeup of the liquid must be the same for the zero calibration and online
readings. Therefore, every time a zero calibration is carried out in the BioTector TOC TN
analyzer, Zero Water must be connected. . Failure to do so may have an impact on system
zero response and the analysis results. The deionised water usage is <500ml per zero
calibration cycle.
Check the electrical connections within the BioTector.
In systems built with FMI Heavy Duty Circulation Pump, insert the fuse for the Circulation
Pump on the Power PCB (Mains PCB).
Reconnect the tube rails/tubing for the Sample, Acid and Base Pumps. Reconnect the tube
rails/tubing for the Nitrogen Pump (N Pump).
Check the Swagelok/PFA tube connections within the BioTector.
Power up the analyzer. Go to Operation, Time & Date menu and adjust the time and the date.
Using the Simulate, Oxidation Phase Simulate menu, check the following:
Check the purity of Oxygen: Turn on the oxygen concentrator or the oxygen supply if a
cylinder is used. When an oxygen concentrator is used, let the concentrator run for at least 10
minutes before the oxygen purity test is carried out.
In the Simulate menu, test the oxygen purity by turning on the MFC with a setting of 10 l/hr,
and flowing gas through the CO
analyzer zero reading should be within ± 0.5% of full scale of the CO
is typically ± 50ppm CO
If the CO analyzer zero reading is outside the specifications, confirm that there is no CO
2
the oxygen by connecting the CO
source and BioTector oxygen inlet and set the MFC to 10 l/h in the same Simulate menu. As
the size of the CO filter is small, keep the 10 l/h gas flow running for at least for 5 minutes
2
and record the CO readings at the end of the 5 minute period. If the CO
2
drop significantly with the CO
contamination in the oxygen supply.
Check that the peristaltic pumps are pumping correctly. The pump rate measurements are used to
confirm that the correct tubing has been installed in the appropriate pump. Any variation between tube
batches is corrected when the zero and span calibration is carried out. The pump rates can be
measured by supplying DI Water or tap water to the pump at the inlet port using a small container.
Confirm the Acid Pump is pumping correctly by removing the fitting at the pump outlet port
and using a 10ml graduated cylinder (at 20 pulses, the measured volume should be between
3.9ml and 4.9ml in ~13 seconds).
Confirm the Base Pump is pumping correctly (at 20 pulses, the measured volume should be
between 3.9ml and 4.9ml in ~13 seconds).
Important Note: For the correct operation of the system, the measured Acid and Base Pump rates
must be identical or as close as possible. The maximum allowable difference in the measured volumes
for acid and base injections above is typically 5% or ~0.2ml. This figure is based on standard system
operation conditions and may not cover systems built with specific configurations.
analyzer for 5 minutes. At the end of this period, the CO
2
. Record the CO analyzer zero reading.
2 2
filter (used with the base container) between the oxygen
2
filter in place, this will indicate that there is no CO
2
analyzer range, which
2
in
2
readings do not
2
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