Operation; Installing The Rotor; Rotor Drive Pin Orientation - Beckman Coulter JA-14.50 Instructions For Use Manual

JA-14.50 Fixed-Angle Rotor
Preparation and Use

Operation

1
Precool the rotor in the centrifuge or in a refrigerator before use—especially before short
runs—to ensure that the rotor reaches the set temperature.
A suggested precooling cycle is a minimum of 30 minutes at 2000 RPM at the required
temperature.
2
If fluid containment is required, use capped tubes.
It is strongly recommended that all containers carrying physiological fluids be capped to
prevent leakage.
3
Select the JA-14.50 rotor.
4
For additional operating information, see the following:
•
Run Times
•
Run Speeds

Installing the Rotor

Rotor Drive Pin Orientation

Two metal pins inside the rotor drive hole engage with teeth on the centrifuge drive hub to prevent
the rotor from slipping during acceleration and deceleration (see
properly installed, the pins sit either in the drive hub grooves or on top of the hub surface
The rotor pins are positioned parallel to the Beckman Coulter name engraved at the center of the
rotor body (see
rotor, also are indicators of the drive pin position. Knowing the pin orientation before you install
the rotor will help to ensure that you position the rotor properly on the hub, minimizing the chance
of hub damage. Follow the steps below.
6
for using k factors to adjust run durations.
for information about speed limitations.
Figure 6). The markings on the lifting ring, located near the top periphery of the
Figure 4). When the rotor is
(Figure
PN B32164AC
5).