Creatinine/Crea - Abbott i-STAT 1 System Manual

CREATININE/CREA

Creatinine is measured amperometrically. Creatinine is hydrolyzed to creatine in a reaction catalyzed by
the enzyme creatinine amidohydrolase. Creatine is then hydrolyzed to sarcosine in a reaction catalyzed
by the enzyme creatine amidinohydrolase. The oxidation of sarcosine, catalyzed by the enzyme
sarcosine oxidase, produces hydrogen peroxide (H
the platinum electrode to produce a current which is proportional to the sample creatinine concentration.
Creatinine Amidohydrolase
Creatinine + H O
2
Creatine Amidinohydrolase
Creatine + H O
2
Sarcosine Oxidase
Sarcosine + O + H O
2 2
H O O + 2H +2e
+
2 2 2
See below for information on factors affecting results. Certain substances, such as drugs, may affect
analyte levels in vivo.
1
If results appear inconsistent with the clinical assessment, the patient sample should be retested using
another cartridge.
INTENDED USE
The test for creatinine, as part of the i-STAT System, is for the quantitative determination of creatinine in
whole blood on the i-STAT handheld.
Creatinine measurements are used in the diagnosis and treatment of renal diseases, in monitoring renal
dialysis, and as a calculation basis for measuring other urine analytes.
Contents
Each i-STAT cartridge contains one reference electrode (when potentiometric sensors are included in
the cartridge configuration), sensors for the measurement of specific analytes, and a buffered aqueous
calibrant solution that contains known concentrations of analytes and preservatives. For cartridges that
contain a sensor for the measurement of creatinine, a list of reactive ingredients is indicated below:
Reactive Ingredient
Creatinine
Creatine Amidinohydrolase
Creatinine Amidohydrolase
Sarcosine Oxidase
Metrological Traceability
The i-STAT System test for creatinine measures creatinine amount-of-substance concentration in the
Rev. Date: 03-Aug-12
Creatine
Sarcosine + Urea
Glycine + Formaldehyde + H
--
Biological Source
N/A
Actinobacillus sp.
Microbial
Microbial
Art: 714183-00T
O ). The liberated hydrogen peroxide is oxidized at
2 2
O
2 2