Venipuncture - Coagulation Tests; Arterial Puncture - General - Abbott i-STAT 1 System Manual

Time to Test

VENIPUNCTURE - COAGULATION TESTS

Blood Flow
Plastic
Time to Test
Repeat Test

ARTERIAL PUNCTURE - GENERAL

Overview
10-4
For the most accurate results, test samples immediately after drawing.
Samples for lactate must be tested immediately. Samples for pH, PCO
TCO , and ionized calcium should be tested within 10 minutes. Other analytes
2
should be tested within 30 minutes.
If testing is not immediate, remix blood collection tubes by gentle inversion at
least 10 times. Roll syringes between the palms for at least 5 seconds each
in two different directions, then invert the syringe repeatedly for at least 5
seconds, and then discard the first two drops of blood. Blood in the tip of the
syringe may have been exposed to air and may not be homogenous with the
sample in the barrel of the syringe. Note It may be difficult to properly remix a
sample in a 1.0 cc syringe
Collection technique resulting in good blood flow must be used. Inadequate
blood flow may produce erroneous results.
The sample for testing should be drawn into a plastic collection device
(syringe or blood collection tube) containing no anticoagulant, clot
activators, or serum/plasma separators. Any transfer device (dispenser,
capillary tube, pipette or syringe) must be plastic and must not contain
anticoagulant.
Samples collected into glass tubes or syringes, or in tubes containing
anticoagulants, activators, or separators cannot be used with the i-STAT
coagulation cartridges.
Note: CLSI guidelines recommend that the sample for coagulation testing be
the second or third tube drawn when using a blood collection system
(use a discard tube if this is the only sample being drawn) or be taken
from the second syringe if a double syringe technique is used for
drawing blood.
The sample must be immediately dispensed into the sample well of the
cartridge and the cartridge must be inserted immediately into an analyzer.
If a repeat measurement is needed, a fresh sample must be obtained.
Arterial punctures are performed to access gas exchange status.
PCO , PO
, and pH values change with changes in ventilatory support at a
2 2
rate dependent on underlying conditions. Sample should be drawn after these
changes have stabilized.
Art: 714372-00L
, PO
,
2 2
Rev. Date: 30-Jul-12