Leica CM1860 Instructions For Use Manual page 41

Clearance angle adjustment
The harder the specimen is, the larger the clearance angle must be.
However: The larger the clearance angle is, the larger the section buckling becomes.
If the clearance angle is too small, sections that are too thick or thin can result. Also consider that
the position of the knife edge relative to the specimen can change when the clearance angle is
adjusted. For this reason, always place the specimen above the knife when adjusting the clearance
angle. Otherwise, the specimen could hit the knife while being raised.
• A clearance angle that is too steep or too shallow may not lead to optimum sectioning results
and can possibly damage the specimen. We generally recommend using higher clearance
angles for harder specimens and relatively lower clearance angles for softer ones.
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Leica CM1860/CM1860 UV – Cryostat
Adjust the clearance angle with every new tis-
sue type!
1. The clearance angle scale is located on the
left side of the blade holder.
2. Release the blade holder by turning the No.
4 Allen screw (23) counterclockwise. Select
a clearance angle of 0°. To do so, align the
number 0 to the index mark (25) and tighten the
25
Allen screw (23). If the sectioning results are
not satisfactory, increase the clearance angle
in 1° increments until you achieve optimum
Fig. 36
results.
7. Daily Use of the Instrument
Settings of 1° - 2° (blade holder CE) are
well suited for most applications.
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