Manual Defrosting Of The Cryochamber; Setting The Section Thickness; Retraction (Factory Default Setting: Disabled) - Leica CM1860 Instructions For Use Manual

6.2.6 Manual defrosting of the cryochamber

Fig. 20

6.2.7 Setting the section thickness

Fig. 21
6.3 Retraction
(factory default setting: disabled)
Enabling retraction:
When switching on the instrument (in the initialization phase), you have to press the
If the handwheel is at the 6 o'clock position or the specimen head is "down", the yellow LED next to
RETRACT is illuminated. When the instrument is switched off, this status is stored.
Disabling retraction:
When switching on the instrument (in the initialization phase), you have to press the
handwheel is at the 6 o'clock position or the specimen head is "down", the yellow LED next to RETRACT
is not illuminated and remains off even during sectioning.
Leica CM1860 /CM1860UV – Cryostat
You can activate manual defrosting of the cryochamber by pressing the
key (continuous tone sounds), followed immediately by the
the cryochamber temperature selection field (continuous tone stops). The
display flashes during the defrosting (duration 12 min.).
To shut off the manual defrost early, press the
by the button in the cryochamber temperature selection field.
Adjust the section thickness using the
control panel field (see Fig. 21) in a range from 1 - 100 µm:
From 1 - 5 µm in 0.5 µm increments
From 5 - 20 µm in 1 µm increments
From 20 - 60 µm in 5 µm increments
From 60 - 100 µm in 10 µm increments
The preselected section thickness appears in the display above the keys.
• Start trimming at approx. 20 µm.
• Decrease the section thickness continually down to the appropriate value.
After changing the section thicknesses, discard the first two or three
sections.
• For sectioning, turn the handwheel at a consistent speed.
6. Operating the Instrument
key, followed immediately
and pushbuttons in the top
key in
key (Fig. 21).
key. If the
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