Leica CM1860 Instructions For Use Manual
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Leica CM1860 Instructions For Use Manual

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Leica CM1860/CM1860 UV
Cryostat
Instructions for Use
English
Order No.: 14 0491 80101 - Revision N
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 2.0, Revision N - 10.2019

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  • Page 1 Leica CM1860/CM1860 UV Cryostat Instructions for Use English Order No.: 14 0491 80101 - Revision N Always keep this manual with the instrument. Read carefully before working with the instrument. Version 2.0, Revision N - 10.2019...
  • Page 3 Only in this way is it possible to continuously improve our technology and manufacturing techniques used in our products. This document is protected under copyright laws. All copyrights to this documentation are held by Leica Biosystems Nussloch GmbH.

  • Page 4: Table Of Contents

    Maintenance ..........................17 Technical Data ......................Installing the Instrument ..................... Installation site requirements ....................22 Unpacking instructions for the Leica CM1860/CM1860 UV cryostat ..........23 Standard delivery - packing list ....................26 Installing the handwheel ......................27 Instrument Setup ....................... Electrical connection .........................28 Preparations for starting up the instrument ................28...
  • Page 5 Cleaning ........................... 71 Disinfection at room temperature....................71 Maintenance ..........................72 9.3.1 General maintenance instructions ....................72 9.3.2 Replacing the fuses ........................74 9.3.3 Replacement of the UVC lamp ....................75 9.3.4 Replacing the LED illumination ....................78 Leica CM1860/CM1860 UV...
  • Page 6 Table of contents Ordering Information, Components and Consumables ............10.1 Ordering information .........................79 10.2 Mobile heat extractor - application ................... 102 10.3 Cold extraction - using the Thermal block ................. 103 10.4 Stationary heat extractor (optional) - application ..............104 10.5 Installing the shelf (movable) (optional) ...................

  • Page 7: Important Information

    Symbols used in the text and their meaning Warning Leica Biosystems Nussloch GmbH assumes no liability for consequential loss or damage due to failure to observe the following instructions, particularly in relation to transportation and package handling, and failure to observe the instructions for handling the instrument carefully.
  • Page 8 Important Information Symbol: Title of the symbol: Date of Manufacture Description: Indicates the date when the medical device was manufactured. Symbol: Title of the symbol: CE Compliance Description: The CE marking is the manufacturer's declaration that the medical device meets the requirements of the applicable EC directives.
  • Page 9 Symbol: Title of the symbol: Store dry Description: Indicates a medical device that needs to be protected from moisture. Symbol: Title of the symbol: Upper Description: Indicates the correct upright position of the package. Leica CM1860/CM1860 UV...

  • Page 10: Qualification Of Personnel

    Qualification of personnel The Leica CM1860/CM1860 UV may only be operated by trained laboratory personnel. Prior to operating the instrument, the operator must carefully read and understand these Instructions for Use and must familiarize him/herself with all technical details of the instrument. The instrument is intended for professional use only.

  • Page 11: Intended Use Of Instrument

    Important Information Intended use of instrument The Leica CM1860/CM1860 UV is a high-performance cryostat with disinfection system (CM1860 UV only) for routine and research applications in the fields of biology, medicine and industry. The instrument is used to freeze and section specimen material quickly for diagnosis. The instrument is not designed for the storage of specimen material. The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in these Instructions for Use.

  • Page 12: Safety

    The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. These Instructions for Use include important instructions and information related to the operating safety and maintenance of the instrument.

  • Page 13: Warnings

    The instrument is equipped with the following safety devices: Handwheel lock and safety guard on the knife holder. Warning CM1860 UV only: To prevent adverse health effects from UVC radiation, the UVC disinfection cycle can be started only after the sliding window has been properly closed. Closing the window activates the corresponding safety features.

  • Page 14: Locking/Unlocking The Handwheel

    Safety Note For current information about certificates and recommendations, visit: http://www.LeicaBiosystems.com 2.3.1 Locking/unlocking the handwheel Warning Rotate the handwheel only if the cryostat is cooled and the cryochamber is cold. Warning Always lock the handwheel prior to manipulating the knife and specimen, changing the specimen or taking a break. To lock the handwheel, move its handle to the 12 o'clock (→ Fig. 1-1) or 6 o'clock...

  • Page 15: Safety Guard

    A stable power supply in accordance with the instrument's specifications is essential to its proper functioning. Failure to comply with the above can damage the instrument. • No additional extension cord may be used. Defrosting Warning The quick freeze shelf can become very hot during the defrosting process! Therefore, do not touch it! Leica CM1860/CM1860 UV...

  • Page 16: Operating The Instrument

    We strongly recommend defrosting the Leica CM1860/CM1860 UV cryostat completely for approx. 24 hours, 1-2x annually or as needed. • It is not necessary to remove the microtome for routine disinfection. The CM1860 UV has been designed for UVC disinfection.

  • Page 17: Removing The Microtome

    Note Please contact Leica Biosystems for further details on adequate disinfection measures. Removing the microtome • The microtome is encapsulated and therefore does not require removal by the user.
  • Page 18 Safety • Turn the instrument off and disconnect the power plug before replacing the lamp. For further information, refer to (→ P. 71 – 9. Cleaning, Disinfection, Maintenance). Warning It is possible to break the UVC lamp during replacement. If this happens, the lamp change must be completed by Technical Service.

  • Page 19: Technical Data

    (width x depth x height) Overall size of device, with 730 x 722 x 1206 mm handwheel (width x depth x height) Working height (armrest) 1025 mm Overall size of packaging (width 960 x 820 x 1400 mm x depth x height) Empty weight (without 135 kg accessories) Leica CM1860/CM1860 UV...
  • Page 20 Technical Data Environmental specification Operating altitude Max. 2000 m above sea level Operating temperature +18 °C to +35 °C Relative humidity (operation) 20 to 60 % RH non-condensing Transport temperature -29 °C to +55 °C Storage temperature +5 °C to +50 °C Relative humidity 10 to 85 % RH non-condensing (transport / storage) Minimum distance to walls Back: 150 mm Right: 300 mm Left: 150 mm Emmisions and boundary conditions Overvoltage category according to IEC 61010-1 Pollution degree...
  • Page 21 Horizontal specimen feed 25 mm +3 mm Vertical specimen stroke 59 mm ±1 mm Specimen retraction 20 μm (can be deactivated) Maximum specimen size 55 mm x 55 mm or 50 mm x 80 mm Specimen orientation ±8 ° (x-, y-axis) Coarse feed Slow: max. 600 μm/s Fast: min. 900 μm/s UVC disinfection (only CM1860UV) Manual start Automatic start Manual abort Duration desinfection cycle Short cycle: 30 minutes Long cycle: 180 minutes Leica CM1860/CM1860 UV...

  • Page 22: Installing The Instrument

    Installing the Instrument Installing the Instrument Installation site requirements Warning Do not operate in rooms with explosion hazard! To ensure proper functioning of the instrument, it must be set up while maintaining a minimum distance on all sides from walls and furniture (→ P. 15 – Transport and installation). The place of installation must meet the following requirements: • The instrument requires an installation area of approx. 600 x 730 mm • Room volume must be at least 8 m • Room temperature consistently 18 °C - 35 °C • Temperature range during storage: 5 °C - 50 °C...

  • Page 23: Unpacking Instructions For The Leica Cm1860/Cm1860 Uv Cryostat

    Installing the Instrument Unpacking instructions for the Leica CM1860/CM1860 UV cryostat Note • When the instrument is delivered, check the tilt indicators (→ "Fig. 3") on the packaging. • If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport. Note this on the shipping documents and check the shipment for possible damage.
  • Page 24 Installing the Instrument Fig. 4 Fig. 5 Fig. 6 Fig. 7 Version 2.0, Revision N...
  • Page 25 • To ensure a secure upright position at its intended location, the instrument must be adjusted. To do so, unscrew both adjusting feet (→ Fig. 8-1) using the provided open-end wrench (size 13) (→ Fig. 8-4). • When subsequently transporting the instrument any further on castors, screw them back in all the way. Fig. 8 Leica CM1860/CM1860 UV...

  • Page 26: Standard Delivery - Packing List

    14 0491 80001 (English, with language CD 14 0491 80200) Compare the delivered components with the parts list and your order. Should you find any discrepancies, please contact your Leica sales office without delay. If the supplied local power cord is defective or lost, please contact your local Leica representative. Note A choice of different knife holders is available for the Leica CM1860/CM1860 UV. Version 2.0, Revision N...

  • Page 27: Installing The Handwheel

    Allen key (6 mm). 4. Attach the cover disk (self-adhesive - not pictured). » To deinstall, proceed in reverse order. Warning Rotate the handwheel only if the cryostat is cooled and the cryochamber is cold. Leica CM1860/CM1860 UV...

  • Page 28: Instrument Setup

    Warning Use only the power cord provided by Leica. Failure to observe this causes an imminently hazardous situation that may result in death or serious injury to the operator or other personnel. • Check that the rubber plug is tight (in the drain hole below the left storage shelf), and press it tightly into the drain if necessary.
  • Page 29 Instrument Setup Warning We do not recommend placing disposable blade dispensers in the cryochamber for pre-cooling because it is possible that individual blades may be stuck together during removal. They pose a risk of injury! Leica CM1860/CM1860 UV...

  • Page 30: General Overview Of The Leica Cm1860/Cm1860 Uv

    Instrument Setup General overview of the Leica CM1860/CM1860 UV Fig. 10 Version 2.0, Revision N...
  • Page 31 Instrument Setup Fig. 11 Leica CM1860/CM1860 UV 13 Specimen disc Waste container 14 Thermal block (optional) Control panel 1 15 Section waste tray Control panel 2 16 Brush shelf Storage shelf, left 17 Orientable specimen head Storage shelf, right 18 Blade/knife holder base (optional)

  • Page 32: Power Switch And Circuit Breaker

    Instrument Setup Power switch and circuit breaker • The switch must be in the top position (switch position 1) (→ "Fig. 12") for switching on the circuit breaker (→ Fig. 12-1). • The switch must be in the bottom position (switch position 0) (→ Fig. 12-2) for switching off the circuit breaker.

  • Page 33: Operating The Instrument

    To lock and unlock the control panel to protect the entered parameters from unintended modifications. Enable/disable the lock by pressing and holding for 5 seconds. UVC disinfection (CM1860 UV only) Note Only CM1860 UV: Before UVC disinfection, move the anti-roll guide to the side to allow complete disinfection. Opening the sliding window interrupts the disinfection cycle. Press the key to resume the disinfection cycle.
  • Page 34 Operating the Instrument Note For information on the lifetime of and on replacing the UVC lamp, refer to (→ P. 75 – 9.3.3 Replacement of the UVC lamp). To start disinfection, the sliding window must be completely closed. • Press the UVC button – 1x briefly, 30 min mode • Press the button – 1x (approx. 4 sec.), 180 min mode Status UVC lamp short disinfection...

  • Page 35: Configuring The Desired Values

    Use the function button labeled with a clock symbol to set the time of day. • Set the current time using the Plus (→ Fig. 15-1) Minus (→ Fig. 15-2) buttons. • Pressing and holding the Plus Minus button continuously increases or decreases the time (auto-repeat function). Leica CM1860/CM1860 UV...

  • Page 36: Setting The Automatic Defrost Time (Cryochamber)

    Operating the Instrument 6.2.2 Setting the automatic defrost time (cryochamber) Fig. 16 The automatic defrost cycle takes place once every 24 hours. • Briefly touch the Plus (→ Fig. 16-1) Minus (→ Fig. 16-2) button to display the beginning of the defrost time that is currently set. The two LEDs between the display of hours and minutes flash at the same time. • To change the beginning of the defrost time in 15-minute increments, touch or press and hold the Plus Minus button.

  • Page 37: Activating The Peltier Element

    • When only 4 minutes are remaining, a dot (4.) appears after the number 4. From this time on, it is safe to shut off the Peltier element early. • To shut it off early, press the button. • After the shutoff, the display returns to (→ Fig. 18-1). Leica CM1860/CM1860 UV...

  • Page 38: Manual Defrosting Of The Quick Freeze Shelf

    Operating the Instrument 6.2.5 Manual defrosting of the quick freeze shelf Warning The quick freeze shelf can become hot during the defrosting process. Therefore, do not touch it! Fig. 19 • You can activate the manual defrost of the quick freeze shelf by pressing the key (→ Fig. 19-1) (continuous tone sounds), followed immediately by the key (→ Fig. 19-2)

  • Page 39: Setting The Section Thickness

    The preselected section thickness appears in the display above the keys. • Start trimming at approx. 20 µm. • Decrease the section thickness continuously until the required thickness is reached. • After changing the section thickness, discard the first two or three sections. • For sectioning, turn the handwheel at a constant speed. Leica CM1860/CM1860 UV...

  • Page 40: Retraction

    Operating the Instrument Retraction Note The factory default setting for the retraction is ON! Enabling retraction: When switching on the instrument (in the initialization phase), press the Plus button (→ Fig. 21-1). If the handwheel is at the 6 o'clock position or the specimen head is "down", the yellow LED next to Retract (→ Fig. 21-3) is illuminated.

  • Page 41: Control Panel 2 - Electric Coarse Feed

    • Fast or slow feed toward the knife starts. The LED (→ Fig. 23-2) starts flashing as long as the specimen head is in motion. The LED lights up when the forward end position has been reached (→ Fig. 23-2). Slow To feed the specimen, press and hold the appropriate Fig. 23 Slow Fast button. Fast Leica CM1860/CM1860 UV...

  • Page 42: Daily Use Of The Instrument

    2. Remove the specimen from the cryostat e.g. fix it for later paraffin embedding. 3. Remove section waste using a cold brush. 4. Start UVC disinfection (→ P. 33 – UVC disinfection (CM1860 UV only)). Specimen freezing • Select the sectioning temperature (cryochamber temperature) according to the tissue type to be sectioned (→ P. 61 – 7.5 Temperature selection chart (in minus °C)).

  • Page 43: Quick-Freeze Shelf

    5. Place the specimen disc in one of the holes of the quick freeze shelf and freeze the specimen at low temperature. 6. Once the specimen is frozen, insert the specimen disc (→ Fig. 25-3) with the specimen into the specimen head (→ Fig. 25-2) and start sectioning. Fig. 24 Leica CM1860/CM1860 UV...

  • Page 44: Specimen Discs

    Daily Use of the Instrument Note If the optional available stationary heat extractor is built in, see (→ P. 104 – 10.4 Stationary heat extractor (optional) - application). Specimen discs 7.3.1 Inserting the specimen discs into the specimen head 1. Lock the handle of the handwheel in the upper position. 2.

  • Page 45: Sectioning

    2. Adjust by screwing clockwise or counterclockwise the countersunk screw (→ Fig. 27-3) in the clamping piece in the base using the Allen key No. 4, so that the eccentric lever (→ Fig. 27-2) can be clamped at 0 ° position and 200 ° position.  Repeat this procedure until the knife holder base is securely clamped and not moving. Leica CM1860/CM1860 UV...

  • Page 46: Clearance Angle Adjustment

    Daily Use of the Instrument Fig. 27 7.4.2 Clearance angle adjustment Note • The harder the specimen is, the larger the clearance angle must be chosen. • However: The larger the clearance angle is, the larger the section buckling becomes. • If the clearance angle is too small, sections that are too thick or too thin can result. Also consider that the position of the knife edge relative to the specimen can change when the clearance angle is adjusted.

  • Page 47: Premium Blade Holder

    Inserting the blade holder CE » Push the upper part of the blade holder onto the base. Clamp the blade holder into place on the left side using an Allen key (No. 4) (→ Fig. 30-23). Leica CM1860/CM1860 UV...
  • Page 48 Daily Use of the Instrument Fig. 30 Inserting the blades into blade holder CE Warning Caution! Microtome blades are extremely sharp! Note The blade holder CE can be used either for the low-profile and high-profile blades. Inserting a high-profile blade Warning The safety gloves included in the (→ P. 26 – 4.3 Standard delivery - packing list) must be worn when inserting the blade! 1. Fold the anti-roll guide system (→ Fig. 31-4) towards the left –...
  • Page 49 – while doing so, hold the lever (→ Fig. 35-11) (not the adjusting screw of the anti-roll guide), so that the height of the anti-roll guide remains unchanged. 2. Open the clamping lever (→ Fig. 35-10) by turning it counterclockwise (→ "Fig. 35"). Leica CM1860/CM1860 UV...
  • Page 50 Daily Use of the Instrument 3. Carefully lift out the blade (→ Fig. 35-9). Continue with instruction step 5. Fig. 35 4. Another option for removing the blade is to use the brush with magnet (→ Fig. 36-12). To do so, fold the clamping lever (→ Fig. 36-10) downward counterclockwise (→ "Fig. 36").
  • Page 51 » The section rolls over the glass insert of the anti-roll guide system (→ Fig. 40-1). L Error: Glass insert not high enough.  Remedy: Turn the knurled nut counterclockwise until the section is pushed between the blade and anti-roll guide as shown in (→ Fig. 40-3). Leica CM1860/CM1860 UV...
  • Page 52 Daily Use of the Instrument Fig. 39 » Sections are compressed and block hits the glass insert (→ Fig. 40-2) after sectioning. L Error: Anti-roll guide system is set too high.  Remedy: Turn the knurled nut clockwise until the section is pushed between the blade and anti-roll guide as shown in (→ Fig. 40-3).

  • Page 53: Blade Holder Ce-Tc

    All 4 longitudinal edges of the anti-roll glass plate can be used. 7.4.5 Blade holder CE-TC The blade holder CE-TC (→ "Fig. 42") is designed only for disposable carbide metal blades (tungsten carbide - TC65). Handling of this holder is identical to that of the blade holder CE (→ P. 47 – 7.4.4 Blade holder CE). Fig. 42 Leica CM1860/CM1860 UV...

  • Page 54: Knife Holder Cn

    Daily Use of the Instrument 7.4.6 Knife holder CN Inserting the base 1. To insert the blade/knife holder base (→ Fig. 43-1), move the lever (→ Fig. 43-2) counterclockwise to the back. Slide the base onto the t-piece (→ Fig. 43-3) of the base plate. Note When inserting the blade/knife holder base, overcome the resistance of a spring (located in the foot of the knife holder) by applying a little pressure towards the left.
  • Page 55 (→ Fig. 45-7) in alternating order until both are tight. Fig. 45 • To remove the knife, follow these instructions in reverse order. • Open the wingscrews by turning them counterclockwise and then pull the knife out to the side. Leica CM1860/CM1860 UV...
  • Page 56 Daily Use of the Instrument Warning • After the knife has been removed from the knife holder, it is stored securely in the knife case (→ "Fig. 46"). NEVER place the knife without the knife case onto the work surface next to the instrument! • To prevent the knife from rust, close knife case when the knife is completely dry.
  • Page 57 1. Use a No. 4 Allen key to loosen the screw over the clearance angle adjustment (→ Fig. 48-23) and remove the segment arc (→ Fig. 48-2) from the blade/knife holder base. Fig. 48 2. Use a No. 4 Allen key to loosen the screws (→ Fig. 49-4) on the underside of the segment arc. Leica CM1860/CM1860 UV...

  • Page 58: Cleaning The Blade And Knife Holders

    Daily Use of the Instrument Fig. 49 Warning Never work with only one clamping jaw, as this does not ensure the stability required for the sectioning process. Also, a long knife will not be sufficiently protected by the safety guard in this instance. 3. Lift off the clamping jaw (→ Fig. 50-5) on the right side (caution: do not lose the washers!) and feed it into the adjacent hole (→ Fig. 50-6).
  • Page 59 The blade holder can now be moved sideways and can be taken out of the segment arc. Fig. 51 Knife holder CN • Occasionally oil the moving parts such as the axes (→ Fig. 52-1) (→ Fig. 52-2) as well as the slot (→ Fig. 52-3) with a drop of cryo oil. Fig. 52 Leica CM1860/CM1860 UV...
  • Page 60 Daily Use of the Instrument Note If several blade/knife holders are cleaned at the same time, the parts must NOT be mixed up! Failure to adhere to this may result in sectioning problems! Disinfection • At room temperature, wipe contaminated surfaces with a paper towel moistened with alcohol-based disinfectants.

  • Page 61: Temperature Selection Chart (In Minus °C)

    Spleen or bloody tissue  Testicular  Thyroid  Tongue  Uterus curettage  The temperature values given in this table are based on experience; however, these are only reference values. Each tissue may require individual temperature adjustments. Leica CM1860/CM1860 UV...

  • Page 62: Defrosting

    Daily Use of the Instrument Defrosting Defrosting the cryochamber actually means defrosting the evaporator to prevent excessive frost buildup. The evaporator is flushed with hot gas during defrosting. The cryochamber itself is not being defrosted. The condensation water that is produced during defrosting is collected in a container that is accessible from the outside. Warning To make sure that the condensation water stemming from the defrost cycles drains into the waste container and to avoid the risk of possible contamination, ensure that the tap of the waste container (→ Fig. 55-2)

  • Page 63: Manual Defrosting Of The Quick Freeze Shelf

    9. Switch off the chamber illumination and start the UVC disinfection. 10. Lock control panel 1 (→ P. 33 – Fig. 14) using the button. 11. Do NOT switch off the instrument using the circuit breaker, as otherwise no cooling takes place. Leica CM1860/CM1860 UV...

  • Page 64: Switching Off The Instrument For A Longer Period

    Daily Use of the Instrument 7.7.2 Switching off the instrument for a longer period Note If you do not intend to use the instrument for several weeks you may turn it off. Please note, however, that it may take up to several hours to cool the cryochamber down to very low temperatures after turning the instrument on again.

  • Page 65: Troubleshooting

    The display alternates between HELP and the real-time display. The message is acknowledged by pressing a key on the keyboard. The HELP display keeps appearing at a relatively short interval. Leica CM1860/CM1860 UV...

  • Page 66: Temperature Control Button

    Troubleshooting Temperature control button On the back of the instrument there is a temperature control button (→ Fig. 54-1). If the temperature of the cryochamber exceeds 60 °C the switch responds automatically and turns the instrument off. Fig. 54 Possible causes and remedies: 1. Temperature of the immediate surroundings is constantly higher than 40 °C.  Drop the temperature of the immediate surroundings. 2. When setting up the instrument, the minimum clearance (→ P. 22 – 4.1 Installation site requirements) to walls and furniture was not kept.

  • Page 67: Possible Sources Of Error, Causes And Remedies

    Scraping noise during sectioning • Anti-roll plate protrudes too far • Align anti-roll plate correctly. and specimen head return beyond the knife edge and is stroke. scraping against the specimen. Leica CM1860/CM1860 UV...
  • Page 68 Troubleshooting Problem Cause Remedy Uneven sections. • Knife damaged. • Use a different part of the knife. • Edge of anti-roll plate • Replace anti-roll plate. damaged. Chatter during sectioning. • Specimen insufficiently frozen • Refreeze specimen onto the onto the specimen disc. disc.
  • Page 69 • Dirt on back of knife. • Clean. • Nick in the cutting edge of the • Use a different part of the blade or knife. blade/knife. Inconsistent or inaccurate • Microtome defective. • Call technical service. specimen feed. Leica CM1860/CM1860 UV...

  • Page 70: Changing The Battery

    Troubleshooting Problem Cause Remedy Specimen disc cannot be • Moisture on the underside • Apply concentrated alcohol to removed. causes the specimen disc to the contact point or heat the freeze to the freezing shelf or specimen head. specimen head. Cryostat non-operational.

  • Page 71: Cleaning, Disinfection, Maintenance

    • Risk of explosion when using alcohol: Provide proper ventilation and ensure the instrument is switched off. Note • Remove frozen section waste from the cryostat with a cold brush daily. • (CM1860 UV only) We always recommend disinfection through UVC-light (→ P. 33 – 6.1 Control panel Disinfection at room temperature Warning • Wear protective clothing during disinfection work (gloves, face mask, lab coat, etc.).

  • Page 72: Maintenance

    To ensure a smooth operation of the instrument over a long period of time we recommend the following: • At least once a year, have the instrument inspected by a qualified service engineer authorized by Leica. • Enter into a service contract at the end of the warranty period. For further information, please contact your local Leica service center. • Clean the instrument DAILY. Version 2.0, Revision N...
  • Page 73 • The germicidal UVC lamp is a simple and safe means of minimizing pathogens, which considerably reduces the risk of infection. • Nevertheless, UVC light disinfection cannot replace chemical disinfection of the cryochamber at regular intervals. • UVC light disinfection is effective only on those surfaces that are directly illuminated. Leica CM1860/CM1860 UV...

  • Page 74: Replacing The Fuses

    Cleaning, Disinfection, Maintenance Fig. 56 Fig. 57 Fig. 58 9.3.2 Replacing the fuses Warning • Turn the instrument off and disconnect the power plug before replacing the fuses! • Use only the fuse types specified in (→ P. 19 – 3. Technical Data). • Failure to comply with this can cause severe damage to the instrument and its surroundings as well as endangering life and limb! Version 2.0, Revision N...

  • Page 75: Replacement Of The Uvc Lamp

    If the lamp is broken, it must be replaced by the service technician, as the replacement involves a high risk of injury. A UVC lamp has an estimated lifetime of approx. 9000 hours. Each on/off switching cycle reduces the lamp life by approx. one hour plus burning time (30 minutes or 180 minutes respectively). Leica CM1860/CM1860 UV...
  • Page 76 Cleaning, Disinfection, Maintenance Note When both disinfection indicator lights (short and long-term disinfection) in control panel 1 are flashing alternately, the UVC lamp must be replaced. 1. Switch off the instrument at the circuit breaker. 2. Disconnect the power plug. 3. Slightly lift the sliding window (→ Fig. 60-1) holding it by the grip (→ Fig. 60-2) and pull it out to the front.
  • Page 77 The UVC lamp (→ Fig. 63-1) is installed in front of the protection screen for chamber illumination (→ Fig. 63-2). 1. Hold the lamp with both hands and carefully pull it out of the clips with a GENTLE forwards motion (→ Fig. 63-3). Fig. 63 Leica CM1860/CM1860 UV...

  • Page 78: Replacing The Led Illumination

    • Dispose of the UVC lamp separately. 9.3.4 Replacing the LED illumination The LED illumination is designed for maximum life span. In case of damage, please contact Leica customer service to arrange replacement. For further details, see (→ P. 105 – 11. Warranty and Service). Version 2.0, Revision N...

  • Page 79: Ordering Information, Components And Consumables

    Knife support for knife holder CN, for short knives 14 0419 19426 Knife support for knife holder CN, for long knives 14 0419 19427 Disposable blades Leica TC-65 disposable blades, 5 pcs. 14 0216 26379 Leica high-profile disposable blades, Type 818, 1 pack of 50 14 0358 38926 Leica high-profile disposable blades, Type 818, 10 packs of 50...
  • Page 80 Ordering Information, Components and Consumables Description Order No. Embedding well bar, 3 x 30 mm 14 0201 39122 Specimen stage, rectangular, 28 mm 14 0201 39123 Specimen stage, rectangular, 36 mm 14 0201 39124 Heat extractor 14 0201 39125 Container for specimen stages 14 0201 39126 Dispensing slides for Dr.
  • Page 81 Knife/blade holder base, for knife holder CN, blade holder CE, CE-TC, premium blade holder Order No. 14 0491 47875 Fig. 66 Premium blade holder, assembly, for high-profile and low-profile blades, with lateral movement, glass anti-roll guide and palm rest Order No. 14 0491 48023 Fig. 67 Leica CM1860/CM1860 UV...
  • Page 82 Ordering Information, Components and Consumables Knife holder CN, for standard microtome knives or magnetic blade rails. Clearance angle adjustment and knife height adjustment. Backrests for long and short knives. Anti-roll guide and adjustable safety guard. Order No. 14 0477 42358 Fig. 68 Blade holder CE, universal (high-profile and low-profile disposable...
  • Page 83 14 0800 37740 Anti-static kit for blade holder CE, low-profile Order No. 14 0800 37739 Fig. 73 Backrest for knife holder CN, for short knives Order No. 14 0419 19426 Fig. 74 Backrest for knife holder CN, for long knives Order No. 14 0419 19427 Leica CM1860/CM1860 UV...
  • Page 84 Ordering Information, Components and Consumables Disposable blades Leica TC-65, Leica TC-65 microtome, disposable blade system for sectioning hard specimen materials. The Leica TC-65 tungsten carbide disposable blades were specially developed for the requirements in labs where hard, blunt materials are routinely sectioned.
  • Page 85 Ordering Information, Components and Consumables Knife, 16 cm, steel, profile c, (flat on both sides, for wax and frozen sections). Serial number of the knife (→ Fig. 78-1) Note: including knife case 14 0213 11140 Order No. 14 0216 07100 Fig. 78 Knife, 16 cm, tungsten carbide, profile c, Note: including knife case 14 0213 11140 Order No. 14 0216 04206 Fig. 79 Leica CM1860/CM1860 UV...
  • Page 86 Ordering Information, Components and Consumables Knife, 16 cm, steel, profile d, Note: including knife case 14 0213 11140 Order No. 14 0216 07132 Knife, 16 cm, tungsten carbide, profile d, Note: including knife case 14 0213 11140 Serial number of the knife (→ Fig. 80-1) Order No. 14 0216 04813 Fig. 80 Variable knife case, For 1 to 2 knives from 10 to 16 cm in length Order No.
  • Page 87 - 4 specimen stages, large - 4 heat extractors - 1 container for specimen stages - 16 dispensing slides Fig. 83 - 1 sectioning board/freezing stage - 1 heat extractor, elevated - 1 embedding forceps, angled Order No. 14 0201 39115 Leica CM1860/CM1860 UV...
  • Page 88 Ordering Information, Components and Consumables Embedding well bar set with small recesses consisting of: - 1 embedding well bar, 18 mm recesses - 4 specimen stages, small - 2 heat extractors - 8 dispensing slides Order No. 14 0201 39116 Embedding well bar set Fig. 84 with medium-sized recesses consisting of:...
  • Page 89 - 28 mm Pack of 1. Order No. 14 0201 39123 Specimen stage, rectangular, stainless steel, size - 36 mm Fig. 87 Pack of 1. Order No. 14 0201 39124 Heat extractor Order No. 14 0201 39125 Fig. 88 Leica CM1860/CM1860 UV...
  • Page 90 Ordering Information, Components and Consumables Container for specimen stages Order No. 14 0201 39126 Fig. 89 Dispensing slides for Dr. Peters' cryoembedding system Pack of 8. Order No. 14 0201 39127 Fig. 90 Version 2.0, Revision N...
  • Page 91 Toolset for cryostats, consisting of: • 1 brush, narrow 14 0183 28642 • 1 Leica brush with magnet 14 0183 40426 • 1 Allen key with handle, No. 5 14 0194 04760 • 1 Allen key, No. 2.5 14 0222 04137 • 1 Allen key, No.
  • Page 92 Ordering Information, Components and Consumables Easy Dip, specimen slide holder, for 12 specimen slides, gray Pack of 6 Order No. 14 0712 40161 Fig. 93 Easy Dip, staining container Color: white Pack of 6 Order No. 14 0712 40150 Color: pink Fig. 94 Pack of 6 Order No.
  • Page 93 25 mm Order No. 14 0416 19275 30 mm Order No. 14 0370 08587 40 mm Order No. 14 0370 08637 55 mm Order No. 14 0419 26491 50 x 80 mm Order No. 14 0419 26750 Fig. 95 Leica CM1860/CM1860 UV...
  • Page 94 Ordering Information, Components and Consumables Fig. 96 Heat extractor, stationary (optional) Brush shelf Peltier element (with 2 startions) Specimen head, orientable Freeze shelf, 8 positions Waste tray Shelf, movable (optional) Storage space Blade holder CE with anti-roll guide - also functions as a safety guard (optional) Version 2.0, Revision N...
  • Page 95 (13 slots) Order No. 14 0491 47786 Fig. 97 Transfer block for specimen discs, small (5 slots) Order No. 14 0491 47787 Fig. 98 Large transfer blocks (→ "Fig. 97") and small transfer blocks (→ "Fig. 98") can be combined (→ "Fig. 99"). Fig. 99 Leica CM1860/CM1860 UV...
  • Page 96 Ordering Information, Components and Consumables Heat extractor mobile (→ P. 102 – 10.2 Mobile heat extractor - application) Order No. 14 0443 26836 Fig. 100 Thermal block for easy removal of frozen tissue from the specimen disc (→ P. 103 – 10.3 Cold extraction - using the Thermal block). Order No. 14 0398 18542 Fig. 101 Adapter for Miles Tissue Tek specimen discs.
  • Page 97 Glass - 70 mm, for special application, consisting • Glass insert 70 mm • Metal frame for exchangeable glass inserts CE • 50 µm spacer Recommended section thickness up to 4 µm For blade holder CE: (14 0491 47873, 14 0419 33992) Fig. 105 Order No. 14 0419 37258 Leica CM1860/CM1860 UV...
  • Page 98 Ordering Information, Components and Consumables Anti-roll guide system CE Glass - 70 mm, for special application, consisting • Glass insert 70 mm • Metal frame for exchangeable glass inserts CE • 150 µm spacer Recommended section thickness over 50 µm For blade holder CE: (14 0491 47873, 14 0419 33992) Order No.
  • Page 99 Blue, 9 x 118 ml Order No. 380 1481 Fig. 110 Both media may not be available in your country. Please contact your local distributor. Embedding medium for cryosectioning Tissue Freezing Medium, 125 ml Order No. 14 0201 08926 Fig. 111 Leica CM1860/CM1860 UV...
  • Page 100 Ordering Information, Components and Consumables Cryo oil 250 ml bottle Order No. 14 0336 06100 Fig. 112 Cut-resistant safety gloves 1 pair, size S Order No. 14 0340 40859 Cut-resistant safety gloves 1 pair, size M Fig. 113 Order No. 14 0340 29011 Version 2.0, Revision N...
  • Page 101 Ordering Information, Components and Consumables Heat extractor stationary, assembly Order No. 14 0471 30792 Low-temperature buffer for heat extractor (parking station) (→ Fig. 114-1) Order No. 14 0471 30793 Fig. 114 UVC fluorescent tube 2-pin Order No. 14 0471 40422 Fig. 115 Leica CM1860/CM1860 UV...

  • Page 102: Mobile Heat Extractor - Application

    Ordering Information, Components and Consumables UVC fluorescent tube 4-pin Order No. 14 0471 57384 Fig. 116 Storage system, movable for installation in the front part of the cryostat for cooled storage of preparation aids. Order No. 14 0491 46750 Fig. 117 10.2 Mobile heat extractor - application Specimen freezing with the freezing shelf can be accelerated by the additional use of a heat extractor.

  • Page 103: Cold Extraction - Using The Thermal Block

    3. After about 20 seconds, the frozen specimen can be removed from the specimen disc with forceps (→ Fig. 118-7). 4. If the cap is too loose, readjust it using the screw (→ Fig. 118-6). Note Do not overtighten the screw. Fig. 118 Leica CM1860/CM1860 UV...

  • Page 104: Stationary Heat Extractor (Optional) - Application

    Ordering Information, Components and Consumables 10.4 Stationary heat extractor (optional) - application • Fasten the support (→ Fig. 119-1) of the heat extractor (→ Fig. 119-2) to the left side panel of the cryochamber using two screws at the provided holes (→ Fig. 119-3) and insert the heat extractor. • Mount parking station (→ Fig. 119-4) into one hole...

  • Page 105: Warranty And Service

    11. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively.

  • Page 106: Decontamination Certificate

    Decontamination Certificate 12. Decontamination Certificate Any product that is to be returned to Leica Biosystems or serviced on site must be properly cleaned and decontaminated. The associated decontamination certificate template can be found on our website www. LeicaBiosystems.com in the product menu. This template must be used to record all required data. If a product is returned, a copy of the completed and signed decontamination certificate must either be enclosed or handed over to a service technician. The user shall be responsible for products that are returned without a completed decontamination certificate or with a missing decontamination certificate.
  • Page 108 Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 69226 Nussloch Germany Tel.: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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