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Record mV or absorbance values from the run for each step (AU1 through AU6, and if
•
applicable, for the quaternary pump, AU7 through AU10) in the AU column of Tables 1
and 2 below.
Table 1 - Binary and Quaternary (Reservoirs A and B)
STEP
TIME
0
5.0
1
1.0
2
3.0
3
3.0
4
3.0
5
3.0
6
3.0
7
5.0
Table 2 - Quaternary (Reservoirs C and D)
STEP
TIME
0
5.0
1
1.0
2
3.0
3
3.0
4
3.0
5
5.0
Comments:
PerkinElmer LAS Representative:
Reviewed By:
Flexar Analytical / FX-10 UHPLC Pump IQ/OQ Rev. D June 2009
FLOW
%A
(water)
5.0
100
5.0
100
5.0
0
5.0
10
5.0
49
5.0
51
5.0
90
5.0
100
FLOW
%C
(water)
5.0
100
5.0
100
5.0
0
5.0
10
5.0
90
5.0
100
CURV
%B
(acetone)
0
0.0
0
0.0
100
0.0
90
0.0
51
0.0
49
0.0
10
0.0
0
0.0
%D
CURV
(acetone)
0
0.0
0
0.0
100
0.0
90
0.0
10
0.0
0
0.0
Date:
Date:
AU
(absorbance or mV)
AU1 =
AU2 =
AU3 =
AU4 =
AU5 =
AU6 =
AU
(absorbance or mV)
AU7 =
AU8 =
AU9 =
AU10 =
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