Analytical Pump (100 Μl Piston) Flow Check @ 1Ml/Min - PerkinElmer FX-10 UHPLC Manual

It is important that the volume delivered is correct; the pressure during the delivery is not
•
critical. There is a minimum pressure requirement of 350 psi for the check valves to
operate properly.
Test Steps
Connect the backpressure regulator to the output of the pump. This device will create the
•
necessary back pressure on the pump check valves.
Purge the pump and fill the solvent line to be used for the test with water.
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Zero the scale. Weigh the collection vessel on a calibrated scale or balance.
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Take the collection vessel and go to the pump and set the pump flow for 1 mL/minute.
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Start the pump. (Direct the initial flow of water off to waste until the pressure stabilizes.
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When pressure stabilizes, direct the flow of water into the collection vessel and
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simultaneously start a stopwatch or other timing device.
Let the pump run for 5 minutes.
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When 5 minutes has elapsed, remove the tube from above the collection vessel and
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insert it into the waste.
Weigh the collection vessel with the water. Subtract the weight of the collection vessel.
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The remaining weight of the water should be 5 grams ± 0 .05 g (40.95 to 5.05 g)
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1 mL/min * 5 min = 5 g (water weighs 1 gram per mL)
Divide the weight of the water by 5 to get the volume delivered in a 1 minute interval.
•
•
Record this number in the Test Result field of Flow Accuracy @ 1 mL/min portion of the
IQOQ Test Certificate. Enter N/A if testing FX-10 UHPLC Pump.
2.4.2 Test Results
Parameter
Analytical Pump (100 µL
Piston) Accuracy: 1
mL/min
2.4.3 Analytical Pump (100 µ µ µ µ L Piston) Flow Check @ 3 mL/min
Test Conditions
Chromatographic Conditions:
•
Test Prerequisites
It is important that the volume delivered is correct; the pressure during the delivery is not
•
critical. There is a minimum pressure requirement of 350 psi for the check valves to
operate properly.
Comments:
PerkinElmer LAS Representative:
Reviewed By:
Flexar Analytical / FX-10 UHPLC Pump IQ/OQ Rev. D June 2009
Specification
± 1% of 1mL/min
(00.99ml/min to
1.01ml/min)
100 % H 2 O
3 mL/min
Test Result Pass/Fail
Date:
Date:
Comments