Pressure Pulsations Test - PerkinElmer FX-10 UHPLC Manual

The Pressure Pulsations Test is to verify that the output flow fluctuations are within
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specification. The specification for this test to pass is 2 % peak-to-peak of the running
pressure at which the test is run.
Test Prerequisites
Start the pump and let the pressure stabilize
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Test Steps
Connect the pump output to a restrictor coil or 1000 psi backpressure device. Set the
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pump flow rate to 1 mL/min. Running pressure for this test must be more than 500 psi.
Adjust tubing length as required to obtain the required back pressure.
Monitor the displayed pressure readout for 1 minute. Note the observed maximum and
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minimum pressures.
Using the numbers obtained determine:
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1.
2.
3.
Example:
Max 1020 psi, Min 1003 psi, Mid Point (MP Pressure) = 1011 psi.
2 % MP = MP * 0.02 = 20.22 psi
Observed Delta = 17 psi
Spec = Observed Delta <= 2 % MP
2.4.1 Test Results
Parameter Specification
Pressure Fluctuation < 2% of MP PSI
2.4.2 Analytical Pump (100 µ µ µ µ L Piston) Flow Check @ 1 mL/min
Test Conditions
Chromatographic Conditions:
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Test Prerequisites
Comments:
PerkinElmer LAS Representative:
Reviewed By:
Flexar Analytical / FX-10 UHPLC Pump IQ/OQ Rev. D June 2009
The difference between maximum and minimum (Delta).
The value of the mid-point pressure (MP).
The 2% value of the midpoint pressure. This mid-point value is the pressure used
to compute what the 2 % peak-to-peak error band should be.
Min Psi
100 % H 2 O
1 mL/min
Max Psi Delta MP
Date:
Date:
Pass/Fail Comments