Test Steps
Connect the backpressure regulator to the output of the pump. This device will create the
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necessary back pressure on the pump check valves.
Purge the pump and fill the line to be used for the test with water.
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Zero the scale. Weigh the collection vessel on a calibrated scale or balance.
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Take the collection vessel and go to the pump and set the pump flow for 3 mL/minute.
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Start the pump. (Direct the initial flow of water off to waste until the pressure stabilizes.
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When pressure stabilizes, direct the flow of water into the collection vessel and
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simultaneously start a stopwatch or other timing device.
Let the pump run for 5 minutes.
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When 5 minutes has elapsed, remove the tube from above the collection vessel and
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insert it into the waste.
Weigh the collection vessel with the water. Subtract the weight of the collection vessel.
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The remaining weight of the water should be 15 grams ± 0 .15 g (14.85 to 15.15 g)
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3 mL/min * 5 min = 15 g (water weighs 1 gram per mL)
Divide the weight of the water by 5 to get the volume delivered in a 1 minute interval.
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Record this number in the Test Result field of Flow Accuracy @ 3 mL/min portion of the
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IQOQ Test Certificate. Enter N/A if testing FX-10 UHPLC Pump.
2.4.3 Test Results
Parameter
Analytical Pump (100
µ µ µ µ L Piston) Accuracy: 3
mL/min
2.4.4 FX-10 UHPLC Pump (30 µ µ µ µ L Piston) Flow Check
@ 0.3 mL/min
Test Conditions
Chromatographic Conditions:
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Test Prerequisites
It is important that the volume delivered is correct; the pressure during the delivery is not
•
critical. There is a minimum pressure requirement of 350 psi for the check valves to
operate properly.
Comments:
PerkinElmer LAS Representative:
Reviewed By:
Flexar Analytical / FX-10 UHPLC Pump IQ/OQ Rev. D June 2009
Specification
± 1% of 3mL/min
(2.97ml/min to
3.03ml/min)
100 % H 2 O
0.3
Test Result
Pass/Fail
mL/min
Comments
Date:
Date: