Calibrate Sample Volumes - Teledyne 5800 Installation And Operation Manual

4.5 Calibrate Sample
Volumes
CALIBRATE VOLUME
DEFINE SAMPLE
CALIBRATE VOLUME:

PRESS WHEN READY!
TAKING ___ ml
CALIBRATE SAMPLE
VOLUME DELIVERED:
___ ml
___! ARE YOU
SURE? YES
5. Return the pump tube to the bulkhead fitting.
Note
If a grab sample is taken while a program is running, it is not
counted as part of the number of samples.
The sampler can deliver sample volumes repeatable to ±5 ml or
±5% of the average volume in a set. For accurate sample
volumes, the sampler uses the suction line length (Section 3.3.4)
to generate pump tables. The pump tables along with the liquid
detector are used to determine the suction head height and
measure the liquid volume. By calculating the suction head, the
delivered volumes are not affected by varying liquid levels.
Note
Incorrect suction line values, disabling the liquid detector, and
worn pump tubes may adversely affect the sample volume
accuracy.
Calibrating the sampler can enhance sample volume accuracy. To
calibrate sample volumes, follow the steps below. These steps
assume that the sampler has been correctly installed (Section 2)
and the suction line has been cut and its length entered in the
sampler configuration (Section 3.3.4). These steps also require a
graduated cylinder to measure the delivered sample volume.
Note
Teledyne Isco offers a 1000 ml graduated cylinder. Order part
number 299-0020-00.
To calibrate the sample volume:
1. At the Main Menu, press the Calibrate button.
2. Select the CALIBRATE VOLUME option and press Enter.
3. Pull the lower pump tube from the bulkhead fitting. Hold
the end of the tube over the graduated cylinder. Press the
Enter button when you are ready.
4. Hold the graduated cylinder while the sampler collects the
sample.
5. Measure the sample volume collected in the graduated
cylinder and enter it in the VOLUME DELIVERED screen.
6. If there is a large difference between the expected volume
and your entered volume, the sampler asks you to confirm
NO
the entered volume. If the volume you entered is correct,
select YES. Otherwise, select NO and re-enter the
measured sample volume.
7. Return the pump tube to the bulkhead fitting.
Isco 5800 Refrigerated Sampler
Section 4 Operation
4-9