Nikon Eclipse LV100POL Instructions Manual page 60

Polarizing microscope
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Problem
The brightness is
insufficient.
(Refer to the
troubleshooting for the
electric system too.)
Diascopic
microscopy
The objective hits the
specimen when
switched from low to
high magnification. The
specimen goes out of
focus by switching
objectives.
The specimen does not
move smoothly.
When viewing through
the binocular eyepiece,
the image does not
resolve into a single
image.
Eye strain develops
while viewing.
58
Cause
The lamp voltage is too low.
A ND filter is placed in the optical path.
The aperture diaphragm is stopped
down too far.
A polarizer or an analyzer is placed in
the optical path although the bright-
field microscopy is intended to be
performed.
The used objective is not suitable for
the microscopy.
The condenser position is too low.
The eyepiece diopters are not adjusted.
The eyepieces are not attached
correctly.
The specimen is placed upside-down.
The cover glass is too thick.
The specimen holder is not secured
correctly on the stage.
The interpupillary distance is not
adjusted.
The eyepiece diopters are not adjusted.
The interpupillary distance is not
adjusted.
The eyepiece diopters are not adjusted.
The brightness is not appropriate.
Eyepieces with different viewfield
numbers are used for the left and right
eyes.
Countermeasure
Adjust the brightness with the
brightness control knob.
Remove the ND filter from the optical
path.
Open the diaphragm to a suitable size.
Remove the polarizer or the analyzer
from the optical path.
Use the designated objective.
Adjust the condenser focus knob so that
the field diaphragm image is focused on
the specimen surface.
Adjust the diopters.
Mount the eyepieces correctly by
aligning the positioning grooves. (P.53)
Set the specimen on the stage with the
cover glass facing up.
Use the specified type of cover glass
(thickness: 0.17 mm).
Secure the specimen holder correctly.
Adjust the interpulillary distance. (P.28)
Adjust the diopters.
Adjust the interpulillary distance. (P.28)
Adjust the diopters.
Adjust the brightness with the
brightness control knob or the
combination of ND filters.(P.23 and 24)
Use eyepieces having the same
viewfield number.
(P.23)
(P.24)
(P.31 and 44)
(P.36 and 37)
(P.49)
(P.30)
(P.28)
(P.28)
(P.28)

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