Nikon Eclipse LV100POL Instructions Manual page 46

3. Aperture Diaphragm in the Epi Illuminator
Since the aperture diaphragm is used for adjusting the
numerical aperture of the illumination system, this
diaphragm is related to the resolution, contrast, and
depth of focus of the optical image. Generally, the
aperture diaphragm should be adjusted to about 70 to
80% of the numerical aperture of the objective.
The aperture diaphragm open/close lever changes the
opening of the aperture diaphragm. Remove one of
the eyepieces, and then adjust the aperture diaphragm
opening while observing the exit pupil of the
objective (the bright area when the aperture
diaphragm is fully opened) in the eyepiece tube.
The diaphragm image may not appear in the case of
samples with low reflectivity. In this case, change to a
sample with a near-polished surface.
* The aperture diaphragm in the LV-UEPI
illuminator was centered before shipping. You
don't need center the aperture diaphragm.
4. Operating Filters on the Epi Illuminator
Two filter sliders are located near the rear side of the
epi illuminator. Each slider can holds two filters.
Push in or pull out the filter slider to locate the filters.
NCB11
(ND filter)
ND4
ND16 (ND filter)
(green interference filter)
GIF
IF (interference filter)
44
Filter type
(color balancing filter)
Intended use
For color balance adjustment and color photomicrography
For brightness adjustment (transmittance 25%)
For brightness adjustment (transmittance 6%)
For contrast adjustment
For interference
Aperture diaphragm
open/close lever
0
P
A. ST O
F. ST O P
B F
D F
Exit pupil of an objective
Ach r
0. 8
0.6 0.90
0.4 JAPAN
0.2
70~80
NC B
ND 8
100
F.S.
Aperture diaphragm
Two filter sliders
0
A. ST O P
P
F. ST O
B F
D F
0. 8 Ach r
0.6 0.90
0.4 JAPAN
0.2
NC B
ND 8
F.S.