Episcopic Microscopy - Nikon Eclipse LV100POL Instructions Manual

22

Episcopic Microscopy

To perform episcopic microscopy, attach the LV-UEPI universal epi illuminator and a lamp for the
epi illumination to the microscope.
1. Switching the Episcopic Illumination
You can switch the illumination between the bright-
field and the dark-field to be used for the episcopic
microscopy by operating the illumination selector
lever on the right side of the epi illuminator.
When the lever is pushed in, the bright-field (BF)
illumination is specified. And when the lever is pulled
out, the dark-field (DF) illumination is specified. But
you cannot perform the episcopic dark-field
microscopy with this microscope. Note that the dark-
field setting (DF) is used for the diascopic
illumination.
Lever position
Push in (BF)
Pull out (DF)
42
Illumination method
Episcopic bright-field illumination
(and Episcopic polarization microscopy)
Diascopic bright-field illumination
(The dark-field microscopy is not available.)
0
P
A. ST O
F. ST O P
B F
D F
Illumination selector lever
Ach r
0. 8 0.90
0.6 JAPAN
0.4
0.2
NC B
ND 8
F.S.