Nikon Eclipse LV100POL Instructions Manual page 22

Rotate the nosepiece to locate the desired
17
magnification objective into the optical
path. Then, perform the microscopy.
• Each time you change objectives, the field
diaphragm and the condenser aperture
diaphragm on the epi illuminator must be
adjusted.
- The field aperture should be adjusted so that
its image circumscribes the view field.
- And the aperture diaphragm should be 70% to
80% of the numerical aperture of the
objective.
See Pages 43 and 44.
• Focus on the specimen again using the fine focus
knob or the coarse focus knob.
See Page 25.
• Use the ND filters on the epi illuminator to
adjust the brightness.
See Page 44.
• To use an oil immersion type objective or a
water immersion type objective, perform the oil
immersion or water immersion.
See Pages 34 and 35.
To perform the episcopic polarization
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microscopy, locate the polarizer on the
side of the epi illuminator into the optical
path and locate the analyzer on the
polarizing intermediate tube into the
optical path.
• The polarizer azimuth should be adjusted with
the ring on the polarizer slider.
See Page 36.
To return to the bright-field microscopy
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• Fully rotate the brightness control knob to the far side to turn off the epi illumination lamp.
• Pull out the illumination selector lever on the epi illuminator to set the dark-field (DF) position for it.
• Push the epi/dia selector switch to select the diascopic illumination. The indicator for the diascopic
illumination (lower side) turns on.
• Rotate the brightness control knob to turn on the diascopic illumination lamp and adjust the
brightness.
• Remove the analyzer out of the optical path.
Turn off all the power after completing the observation.
20
20
17
0
B F
D F
Switch to
17
any
desired
objective.
Ach r
0. 8 0.90
0.6 JAPAN
0.4
0.2
F.S.
17
18
0
B F
D F
Locate the polarizer
18
into the optical path.
0. 8 Ach r
0.90
0.6 JAPAN
0.4 0.2
F.S.
Adjust the field
diaphragm and the
aperture diaphragm.
A. ST O P
F. ST O P
NC B
ND 8
Readjust the focus.
Locate the analyzer
into the optical path.
P
A. ST O
P
F. ST O
NC B
ND 8