Oil Immersion Operation - Nikon Eclipse LV100POL Instructions Manual

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Oil Immersion Operation

Objectives marked "oil" are oil-immersion type
objectives. These objectives are used with immersion oil
(option) between the specimen and the tip of the objective.
Bubbles in the oil will adversely affect the viewing of the
image. Be careful to prevent bubbles from forming. To
check for air bubbles, fully open the field diaphragm and
aperture diaphragm, remove the eyepiece, and examine the
pupil (bright round section) of the objective inside the
eyepiece tube. If it is difficult to ascertain the presence of
bubbles, attach an optional centering telescope with an optional adapter, then check for air bubbles
while turning the eyepiece section of the centering telescope to adjust focus. If you detect bubbles,
remove them by one of the following methods:
• Turn the nosepiece slightly to move the oil-immersed objective back and forth once or twice. (In
the case of the condenser, gently turn the condenser focus knob to move the condenser up and
down slightly.)
• Apply more oil.
• Remove the oil and replace it with new oil.
Use as little oil as possible (just enough to fill the space between the tip of the objective and the
specimen, or between the tip of the condenser and the specimen). If too much oil is applied, the
excess oil will flow onto the stage or around the condenser.
Wipe off oil
Any oil remaining on the oil-immersion objective or adhering to the dry-type objective will
noticeably degrade image quality. After use, thoroughly wipe off all oil, and make sure that no oil
remains on the tips of other objectives. Oil on the condenser should also be wiped away carefully
after use.
Use petroleum benzine to wipe off immersion oil. For optimum results, we recommend following
up petroleum benzine with absolute alcohol (ethyl or methyl alcohol).
If petroleum benzine is unavailable, use methyl alcohol alone. However, methyl alcohol does not
clean as well as petroleum benzine, it will be necessary to wipe the surface repeatedly. (Usually,
three- or four-times wipe is sufficient to clean the lenses.)
WARNING
When using petroleum benzine or absolute alcohol, always follow the instructions provided
by the manufacturer. These liquids are highly flammable and must be kept away from
flames and sparks.
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