Bertrand Lens Operation - Nikon Eclipse LV100POL Instructions Manual

20

Bertrand Lens Operation

The polarizing intermediate tube has the Bertrand lens. The Bertrand lens can be placed into the
optical path to perform the conoscope observation.
Setting the Bertrand lens
Put the Bertrand lens turret in the "B" position to move the
Bertrand lens into the optical path.
Put the Bertrand lens turret in the "0" position to remove the
Bertrand lens from the optical path.
Focusing and centering the Bertrand lens
The objective pupil positions vary by magnification and type. So, each time objectives are
switched, the Bertrand lens must be adjusted.
Besides, the Bertrand lens must be centered so that it is aligned to the optical path of the objective.
Note that you need not center the Bertrand lens each time in the case that you have centered the
objective already. (Page 33)
In this adjustment, the aperture diaphragm image is used in the same manner as the condenser lens
adjustment. Do as follows:
1 Perform steps in Page 12, "1 Diascopic Bright-Field Microscopy," and focus on the
specimen.
And focus and center the condenser. (Page 30)
2 Move the Bertrand lens into the optical path.
3 Stop down the aperture diaphragm of the condenser to get a diaphragm image into view.
4 Perform the focusing for the Bertrand lens.
Adjust the Bertrand lens focus ring on the
polarizing intermediate tube to get a clear image
of the diaphragm.
5 Fully stop down the aperture diaphragm of the
condenser.
6 Center the Bertrand lens.
Rotate two centering screws on the polarizing
intermediate tube so that the diaphragm image
comes to the center of the field of view.
III. Operation of Each Part
0
Bertrand
lens turret
Bertrand lens
centering screws
(both sides)
Bertrand
lens focus 0
ring
39