Orthoscopic Observation - Nikon Eclipse LV100POL Instructions Manual

2

Orthoscopic Observation

This section describes the orthoscopic observation procedure. This observation method is the characteristic
method for polarizing microscopes. In this method, the specimen is observed with the polarizer and the
analyzer placed in the optical path.
The shape of the specimen in the direction of the optical axis and its optical properties in the direction of the
thickness can be observed. The vibration direction of the light and the property of the refraction can be
measured with observing light extinction or interference colors of specimens using the circular graduated stage.
Focus on the specimen with the diascopic bright-field microscopy. (Refer to Page 12,
1
"1. Diascopic Bright-field Microscopy.")
Pull out the analyzer slider of the
2
polarizing intermediate tube to locate the
analyzer into the optical path.
Set the scale for the analyzer to the "0" position.
And, remove the Bertrand lens from the optical
path.
See Pages 37 and 39.
Set the polarizer for the diascopic
3
microscopy into the polarizer rotation ring.
See Page 36.
Adjust the orientation of the polarizer and
4
the analyzer.
See Page 38.
After the adjustment, observe the specimen and
focus on it again.
Perform the orthoscopic microscopy.
5
• You can measure the retardation in various range with the P-CL 1/4 λ & tint plate.
See Page 40.
• The condenser aperture diaphragm and the field diaphragm must be adjusted in the same ways for the
bright-field microscopy.
See Pages 31 and 32.
• The top lens of the swing-out condenser must be operated depending on the magnification of the
objective to be used.
See Page 29.
16
0
0. 8 Ach r
0.90
0.6 JAPAN
0.4 0.2
F.S.
Set the polarizer.
3
Locate the
2
analyzer into
the optical path.
NC B
ND 8