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GE Monitor UPC-900 Operating Instructions Manual
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GE Monitor UPC-900 Operating Instructions Manual

GE Monitor UPC-900 Operating Instructions Manual

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Monitor UPC-900
Operating Instructions
Original instructions

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Summary of Contents for GE Monitor UPC-900

  • Page 1 Monitor UPC-900 Operating Instructions Original instructions...
  • Page 2 Page intentionally left blank...
  • Page 3 Filter noise in the UV signal ......................Read pH, temperature and conductivity ................... Monitor check ............................5.10 Monitor setup ............................5.11 Alarm timer .............................. 5.12 Service displays ............................. Maintenance ......................Cleaning before planned service ....................Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 4 Conductivity flow cell .......................... pH electrode ............................Storage ..............................Troubleshooting ....................Fault actions ............................Error messages ............................Reference information ..................Specifications ............................Menu overview ............................Ordering information .......................... Health and Safety Declaration Form ................... Index ........................Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 5 The Operating Instructions provides you with the instructions needed to handle Monitor UPC-900 in a safe way. Prerequisites In order to operate Monitor UPC-900 safely, and according to the intended purpose, the following prerequisites must be met: • You should be acquainted with the use of bioprocessing equipment and with the handling of biological materials.
  • Page 6 If you do, you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment. Intended use Monitor UPC-900 is intended for research use only, and shall not be used in any clinical procedures, or for diagnostic purposes. Safety notices This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product.
  • Page 7 Hardware items are identified in the text by bold text (for example, Power). Regulatory information In this section This section describes the directives and standards that are fulfilled by Monitor UPC-900. Manufacturing information The table below summarizes the required manufacturing information. For further infor- mation, see the EU Declaration of Conformity (DoC) document.
  • Page 8 • connected to other products recommended or described in the user documentation, • used in the same state as it was delivered from GE, except for alterations described in the user documentation. International standards This product fulfills the requirements of the following standards:...
  • Page 9 Regulatory compliance of connected equipment Any equipment connected to Monitor UPC-900 should meet the safety requirements of EN/IEC 61010-1, or relevant harmonized standards. Within EU, connected equipment must be CE marked.
  • Page 10 1.4 User documentation User documentation In addition to these Operating Instructions, the documentation package supplied with Monitor UPC-900 also includes product documentation binders containing detailed specifications and traceability documents. The most important documents in the document package with regard to technical aspects of Monitor UPC-900 are listed below.
  • Page 11 Important WARNING Before installing, operating or maintaining Monitor UPC-900, all users must read and understand the entire contents of this chapter to become aware of the hazards in- volved. Failure to do this may cause human injury or death, or damage to the equipment.
  • Page 12 System operation, on page 14. • Maintenance, on page 14. General precautions Always follow these General precautions to avoid injury when using the Monitor UPC-900 instrument. WARNING Do not operate the product in any other way than described in the ManualNameShort.
  • Page 13 Follow local and/or national regulations for safe operation and maintenance of Monitor UPC-900. WARNING Spread of biological agents. The operator has to take all necessary actions to avoid spreading hazardous biological agents.
  • Page 14 Do not connect/disconnect the lamp with the monitor switched Maintenance WARNING Electrical shock hazard. All repairs should be done by service personnel authorized by GE. Do not open any covers or replace parts unless specifically stated in the user documentation. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 15 When lamp power is on, the lamp socket carries dangerous voltage. Do not connect/disconnect the lamp with the monitor switched WARNING Use only GE parts. Only spare parts and accessories that are ap- proved or supplied by GE may be used for maintaining or servicing the product.
  • Page 16 Labels Introduction This section describes the system label on Monitor UPC-900 and its meaning. System label The illustration below shows the system label. Note: The specific data shown on the system label below is only an example. Actual data is specific for each individual system and may vary from system to system.
  • Page 17 NRTL means an organization, which is recognized by the US Occupational Safety and Health Administration (OSHA) as meeting the legal requirements of Title 29 of the Code of Federal Regulations (29 CFR), Part 1910.7. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 18 2.3 Emergency procedures Emergency procedures Introduction This section describes how to perform an emergency shutdown of Monitor UPC-900. It also describes the result in the event of power failure. Emergency shutdown In an emergency situation, do as follows to stop the run: Switch off power to the instrument by pressing the Power switch (1) on the rear panel to the 0 position.
  • Page 19 Monitor UPC-900 shall be decontaminated before decommissioning and all local regu- lations shall be followed with regard to scrapping of the equipment. Disposal, general instructions When taking Monitor UPC-900 out of service, the different materials must be separated and recycled according to national and local environmental regulations. Recycling of hazardous substances Monitor UPC-900 contains hazardous substances.
  • Page 20 Waste electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 21 Periodic replacement of those consumables or parts to maintain the declared EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 22 Indicates that this toxic or hazardous substance contained in at least one of the homoge- neous materials used for this part is above the limit requirement in SJ/T11363-2006. Data listed in the table represents best information available at the time of publication. • Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 23 System description Module Monitor UPC-900 is an on-line monitor for measurement of UV-absorption, pH (optional in ÄKTA™ systems) and conductivity. The monitor can work with standard glass pH electrodes with a built in liquid-filled reference electrode and a BNC connector.
  • Page 24 3 System description 3.1 Illustrations Illustrations Monitor UPC-900 front and right side Part Function Display Dial Holder Optical unit Conductivity flow cell pH electrode Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 25 UV signal from optical unit Analogue out 0 1 V Chart recorder outputs, 3 channels 0 to1.0 V UniNet 1 Computer network Power switch Mains supply on/off switch Mains Supply voltage, grounded Lamp UV lamp connection Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 26 3 System description 3.2 Optical unit Optical unit Illustration Part Function Optical unit Filter Lens Beam splitter UV flow cell Inlet Photodetector Outlet Photodetector Analog outputs Microprocessor Monitor UPC-900 front panel Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 27 The conductivity is automatically calculated by multiplying the measured conductance by the flow cell’s cell constant. The cell constant is precalibrated on delivery but can be measured with a separate calibration procedure. This procedure is described in Setup adjust conductivity, on page 74. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 28 Conductivity measurement has a dynamic range from 1 μS to 999.9 mS/cm. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 29 Ag/AgCl, an outer electrolyte bridge of 1 M KNO3, an annular ceramic reference junction and a low profile pH membrane. The pH electrode is delivered with a transparent cover. The flow cell should not be used with any other pH electrode. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 30 Internal electrolyte bridge containing 4M KCl saturated with AgCl Silicone sealing Ag/AgCl reference electrode Internal annular coaxial ceramic reference junction External electrolyte bridge containing 1M KNO O-ring Glass electrode containing diluted, buffered KCl O-ring External annular coaxial ceramic reference junction Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 31 4 Installation Installation About this chapter This chapter provides required information to enable users and service personnel to unpack, install, move and transport Monitor UPC-900. Precautions WARNING Before attempting to perform any of the procedures described in this chapter, you must read and understand all contents of the Safety instructions chapter.
  • Page 32 To ensure correct ventilation a free space of 0.1 m is required behind and in front of the instrument. Do not use any soft material under the instrument, to ensure that the venti- lation inlet in the front is not blocked. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 33 ÄKTA systems. Install the optical unit Install the holder Hook the holder (1) into the slot on the right hand side of the module. Secure it by pushing the slide clamp up (2). Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 34 4 Installation 4.3 Install the optical unit Illustration of the UV flow cell Part Function Flow cell 5 mm O-ring Flow cell 2 mm O-ring Protective plug for flow cell (red) Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 35 To remove the flow cell, reverse the procedure. Note: Make sure that the Hg lamp position and the filter is selected according to the wave length to be used. This is described in Change lamp assembly, on page 92. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 36 Secure it by tightening the screw (2) in the holder. Connect the lamp cable to the socket Lamp on the rear panel of the instru- ment. Connect the signal cable to the socket Optical unit on the rear panel of the instrument. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 37 The length should be 170 mm when using the 5 mm flow cell, and 230 mm when using the 2 mm flow cell. Connect the other end of the tubing to the conductivity flow cell or to another appropriate unit. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 38 Connect the conductivity flow cell to the socket conductivity flow cell on the rear panel of the module. Connect the tubing with fingertight connectors. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 39 If the flow cell holder is not used, the flow cell must still be installed at an angle of 30° from the vertical with the outlet placed higher than the inlet to prevent air bubbles being trapped in the cell. The flow direction is marked on the flow cell. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 40 4 Installation 4.5 Install the pH electrode Step Action Connect the tubing with fingertight connectors. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 41 If the flow cell is full of liquid, it is not possible to insert the electrode. If so, loosen the inlet connection while inserting the electrode to allow the liquid to run out from the flow cell. Remember to re-tighten the connector. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 42 The sockets for electrical signals are located on the rear panel. The monitor and the flow cells are separate units. All flow cells are connected to the rear of Monitor UPC-900, see illustration in Rear side, on page 25.
  • Page 43 The signal cable is delivered with protective covers on each wire. Do not remove the protective covers from unused connections as a short circuit may dis- turb the measurements. Set the recorder to 0 to 1 V input, full scale. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 44 PC and a termination plug. The UniNet 1 link can connect, in series, the PC with Pump P-920, Monitor UPC-900 and the Frac-920. The termination plug is connected to the last module in the chain.
  • Page 45 Remove the four screws in the filter housing. Separate the filter housing from the detector housing. Carefully remove the filter wheel from the filter housing. If necessary, remove the filter(s) from the filter wheel by pressing it (them) out, for example with a small screwdriver. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 46 Reassemble the circular plastic band with the filter wheel peg fitting into the band notch. Check that all filters are clean. Insert the filter wheel back into the filter housing. Note: The filter wheel can be placed only in correct position. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 47 4 Installation 4.7 Optical filters (optional) Step Action Reassemble the filter housing with the detector housing by fastening the four screws. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 48 5 Operation Operation About this chapter This chapter provides the information required to operate Monitor UPC-900 in a safe way. Precautions WARNING Before attempting to perform any of the procedures described in this chapter, you must read and understand all contents of the Safety instructions chapter.
  • Page 49 The selftest takes approx. 1 minute. When start-up is completed with no errors, the display shows the main menu 1. The monitor can be used immediately but the full specifications are not obtained until after 1 hour of lamp warm–up. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 50 ÄKTA systems. Note: When running chromatography using organic solvents, we recommend that the pH electrode is removed and the dummy electrode inserted in its place as organic solvents will cause pH electrode degeneration. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 51 When the required menu is visible, the menu or selection is accepted by pressing the OK button. OK button Go down to a sub menu or accept setting. ESC button Return one menu level. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 52 The cursor moves back one place to the right every two seconds if the dial is not turned. The text or numerical value displayed is accepted by pressing OK. To cancel, press ESC. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 53 12.50 22.4 Set Cond Zero Level Set UV Range Calibrate pH Set ph Analog. Out Check Monitor UPC-900 Operating Instructions 29-0567-16 AB (7.00–12.00) Alarm/Timer 00:22:24 Eventmark Set Cond Analog. Out Set UV Analog. Out Set Parameters Set UV Zero Level...
  • Page 54 Set UV Range Check Set UV Zero Level Set Cond Full Scale Calibrate pH Setup Set ph Analog. Out (7.00–12.00) Monitor UPC-900 Operating Instructions 29-0567-16 AB Calibr pH Buffer 1 Alarm/Timer 00:22:24 Set ph Full Scale Calibr pH Buffer 2...
  • Page 55 Set UV Range Check Set UV Zero Level Set Cond Full Scale Setup Calibrate pH Set ph Analog. Out (7.00–12.00) Monitor UPC-900 Operating Instructions 29-0567-16 AB Alarm/Timer 00:22:24 Calibr pH Buffer 1 Set ph Full Scale Calibr pH Buffer 2...
  • Page 56 12.50 22.4 Autozero UV Cond%Tc pH Check Set Cond Zero Level Set UV Range Alarm/Timer 00:22:24 Monitor UPC-900 Operating Instructions 29-0567-16 AB Set Cond Analog. Out Set UV Analog. Out Eventmark Lamp Set Parameters (on) Setup Set UV Zero Level...
  • Page 57 Set UV Zero Level Set Parameters Setup Set Cond Zero Level Set UV Range Calibrate pH Set Cond Full Scale Monitor UPC-900 Operating Instructions 29-0567-16 AB (7.00–12.00) Check Alarm/Timer 00:22:24 Set UV Analog. Out Set Cond Analog. Out Calibr pH Buffer 1...
  • Page 58 Set ph Zero Level Set UV Analog. Out Set Cond Analog. Out Set UV Zero Level Calibr pH Buffer 2 Set ph Analog. Out Monitor UPC-900 Operating Instructions 29-0567-16 AB Setup Set Cond Zero Level Calibrate pH Set Cond Full Scale Calibration OK (7.00–12.00)
  • Page 59 Adjustment of the cell constant is only necessary when the monitor is to be used to de- termine conductivity with high accuracy. The procedure is described in Setup adjust conductivity, on page 74. Calibration can also be performed from UNICORN. Select System Control:System:Calibrate. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 60 Lamp (on) Select Set parameters and press OK. Cond%Tc pH Calibrate pH Autozero UV (7.00–12.00) Lamp Eventmark (on) Cond%Tc pH Autozero UV pH 12.50 22.4 Lamp Eventmark (on) Monitor UPC-900 Operating Instructions 29-0567-16 AB Autozero UV pH 12.50 22.4 Check...
  • Page 61 Cond%Tc pH Set Cond Zero Level Set UV Range Set Cond Full Scale pH 12.50 22.4 Calibration OK Monitor UPC-900 Operating Instructions 29-0567-16 AB Check Alarm/Timer 00:22:24 Lamp Set UV Analog. Out Set Cond Analog. Out Set UV Zero Level...
  • Page 62 100% corresponds to 59.16 mV per pH step at 25°C. The asymmetry potential at pH 7 is shown as a mV value. Press ESC to return to the Set Parameters menu. Before use, rinse the electrode using distilled water. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 63 An electrode is still usable at lower slopes and higher asymmetry potentials but the re- sponse will be slower and the accuracy diminished. Voltage (mV) +500 mV 0 mV –500 mV Part Function Voltage (mV) Assymmetry Calibration curve Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 64 Peaks narrower than the minimum peak width value according to the table below may be distorted. Because of this, the averaging time should be as short as possible. On de- livery, the averaging time is set to 1.3 s. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 65 Set Cond Full Scale Calibrate pH Calibr pH Buffer 2 Set ph Analog. Out (7.00–12.00) Monitor UPC-900 Operating Instructions 29-0567-16 AB Set ph Zero Level Set UV Analog. Out Set Cond Analog. Out Set UV Zero Level Calibr pH Buffer 1...
  • Page 66 Calibrate pH (7.00–12.00) Set ph Full Scale Set UV Analog. Out Set Cond Analog. Out Calibr pH Buffer 1 Monitor UPC-900 Operating Instructions 29-0567-16 AB Set ph Zero Level Set UV Range Set Cond Full Scale Calibr pH Buffer 2...
  • Page 67 Select main menu Check and press OK. Check Lamp Intensity Select sub menu Check Lamp Intensity. Check Recorder Check Recorder Please wait! Check Recorder Check Recorder Please wait! Check Service Mode Check Service Mode Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 68 10% steps back to 0 V. The test is run continuously. Compare the diagram of the chart recorder with the figure. Check Service Mode Check Service Mode Stop the test by pressing OK or ESC. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 69 The module name and software version are displayed, UPC-900 press OK. V1.00 The date of the last service is displayed, press OK. Date of Maintenance: A test of the module’s buzzer is performed, press OK. Buzzer Test Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 70 Select sub menus Temp, Cond, pH or UV with the dial Setup and press OK. Temp Cond pH UV Setup Temp Cond pH UV Setup Adjust Temp (22.3°C) Warning! This will change temp calib Setup Adjust Temp (22.3°C) 22.3 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 71 The current adjustment value is displayed as default. Setup Adjust Temp Enter the temperature shown on the thermometer and Setup (22.3°C) 22.3 Temp Cond pH UV press OK. Setup Cond Ref Temp (25.0°C) Setup Cond Ref Temp (25.0%) 25.0 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 72 OK. Setup Cond Ref Temp Setup Setup Cond Ref Temp (25.0°C) Temp Cond pH UV (25.0%) 25.0 Setup Cond Ref Temp Setup Cond Ref Temp (25.0°C) (25.0%) 25.0 Setup Cond Ref Temp (25.0%) 25.0 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 73 The current reference temperature value is displayed Setup Cond Ref Temp as default. Adjust the reference temperature value Setup Cond Ref Temp (25.0%) 25.0 (25.0°C) setting as necessary and press OK. Setup Cond Ref Temp (25.0%) 25.0 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 74 Warning! This will pressing OK. Setup Cond Ref Temp change cell calib (25.0°C) Warning! This will change cell calib Setup Cond Ref Temp (25.0%) 25.0 Setup Adjust Cond Monitor UPC-900 Operating Instructions 29-0567-16 AB (83.53mS/cm) 86.6 Setup Adjust Cond (83.53mS/cm) 86.6...
  • Page 75 (83.53mS/cm) 86.6 and press OK. The new cell constant is automatically calculated. The range is 1.000 to 999.9 mS/cm. Conductivity graph The graph shows the conductivity of 1.00 M NaCl at 20 to 30°C. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 76 Temp Cond pH UV Setup Show Cond (on) Setup Cond Ref Temp Change the setting if desired and press OK. (25.0°C) Setup Show Cond (on) on off Setup Cond Ref Temp (25.0%) 25.0 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 77 Temp Cond pH UV Setup Setup Set Averaging Temp Cond pH UV Temp Cond pH UV (1.3S) Set Averaging Set Averaging (1.3S) (1.3S) Set Averaging (1.3S) 0.64 Set Averaging Set Averaging (1.3S) 0.64 (1.3S) 0.64 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 78 (1.3s) 0.64 Lamp (on) Autozero UV Setup Cond%Tc pH Temp Cond pH UV Setup Adjust Temp Eventmark Lamp (22.3°C) (on) Monitor UPC-900 Operating Instructions 29-0567-16 AB pH 12.50 22.4 Warning! This will Autozero UV change temp calib Setup Adjust Temp...
  • Page 79 Temp Cond pH UV Setup Language (GB) GB D E F I Setup Adjust Temp Setup Language (22.3°C) (GB) GB D E F I Warning! This will change temp calib Monitor UPC-900 Operating Instructions 29-0567-16 AB Setup Adjust Temp (22.3°C) 22.3...
  • Page 80 I = Italian Setup unit number The unit number is the identification the Monitor UPC-900 has on the UniNet-bus. It should correspond to the number set in UNICORN for the Monitor UPC-900. The number should be set to 0 if one Monitor UPC-900 is used.
  • Page 81 Sets the display angle to compensate for different viewing heights. Step Action Display Select main menu Setup and press OK. Select sub menu Set Display Angle and press OK. Set Display Angle Select viewing angle (->\ Up, ->| Mid or ->/ Down). Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 82 Set Timer menu. The clock will be reset when Buzzer 18:34:52 power is turned off. A set alarm/timer function can be reset by pressing OK in the menu Alarm/ Timer off? Insert Access Code: Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 83 5.12 Service displays Insert access code The instrument has service displays for use by authorised service personnel. If the service display Insert Access Code is accidentally selected, press ESC to return to the normal operation display. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 84 GE. Do not open any covers or replace parts unless specifically stated in the user documentation. WARNING Use only GE parts. Only spare parts and accessories that are ap- proved or supplied by GE may be used for maintaining or servicing the product.
  • Page 85 Copy the form you need from Section 8.4 Health and Safety Declaration Form, on page 118 or print it from the PDF file available on the User Documentation CD. User maintenance schedule Periodic maintenance Interval Action Every 3 months Check the monitor Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 86 Follow local and/or national regulations for safe operation and maintenance of Monitor UPC-900. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 87 Rinse the syringe and then flush the flow cell with distilled water (10 ml). 6.3.3 Conductivity flow cell Precautions WARNING Corrosive substance. NaOH is corrosive and therefore dangerous to health. When using hazardous chemicals, avoid spillage and wear protective glasses and other suitable Personal Protective Equipment (PPE). Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 88 Follow local and/or national regulations for safe operation and maintenance of Monitor UPC-900. Clean the conductivity flow cell inplace...
  • Page 89 If these procedures fail to rejuvenate the electrode, the problem is most likely a clogged liquid junction. Step Action Heat a 1 M KNO solution to 60 to 80°C. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 90 R <300 mV for 254 nm R <150 mV for 280 nm R <150 mV for 214 nm, Cond%Tc pH replace the lamp according to Change lamp assembly, on page 92, or contact GE for lamp replacement. Lamp (on) Autozero UV Eventmark pH 12.50...
  • Page 91 • Lamp room temperature. (on) When necessary replace the lamp according to Change Autozero UV lamp assembly, on page 92, or contact GE for lamp re- placement. Eventmark pH 12.50 22.4 Autozero The module internal absorbance value for autozero can be checked to test the consis- tency of buffers.
  • Page 92 Slide the lamp housing firmly into place. There will be a faint click when the housing is positioned correctly. The Hg lamp housing can take up two positions, one for 280 nm and one for all other wavelengths. Note: The Zn lamp housing has only one position. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 93 The Hg optics with 254 and 280 nm filters and the Zn optics with the 214 nm filter are delivered with filters installed. If other filters are to be used, install the new filters as de- scribed in Install optical filters, on page 45. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 94 The flow cell can also be stored dry by flushing as above with distilled water and then blowing a compressed inert gas such as nitrogen (N ) through the cell. Replace the red protective caps. Never use compressed air as this may contain droplets of oil. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 95 (supplied) can be inserted in the flow path. Electrode regeneration If the electrode has dried out, immerse the lower end of the electrode in buffer with a 1:1 mixture of pH 4 buffer and 2 M KNO overnight. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 96 This chapter provides information required to enable users and service personnel to identify and correct problems that may occur when operating Monitor UPC-900. If the suggested actions in this guide do not solve the problem, or if the problem is not covered by this guide, contact your GE representative for advice.
  • Page 97 Clean the system in accordance with the ÄKTA • system manual. Clean the column in accordance with the column • instructions. Check that the mixer is functioning correctly and • that the correct chamber volume is being used. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 98 • be restored by soaking it in buffer overnight. Clogged liquid junction. Refer to Section 6.3.4 Clean • the pH electrode, on page 89. pH values vary with varied Replace the pH electrode. back-pressure Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 99 We recommend that the pH electrode is not used in applications using organic solvents. Mount the dummy electrode instead. Clogged liquid junction. Refer to Section 6.3.4 Clean • the pH electrode, on page 89. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 100 Section 6.3.2 Clean UV flow cell, on page 86. buffer appears over time The ambient temperature may have changed. Use • a temperature compensation factor, see Setup conductivity temperature compensation, on page72. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 101 Set the conductivity analog output, on page 56 and Check recorder, on page 68 No text on the front dis- Check that the mains cable is connected and the play power switch is in ON-position. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 102 7 Troubleshooting 7.2 Error messages Error messages Warnings 34 to 77 If the suggested actions do not correct the fault, call GE. Message Action 1 Perform a new start-up. The preceding message 34 Start up failed may tell more about the cause.
  • Page 103 3 Check that optical unit is not exposed to direct sunlight. 4 If problem remains, call service 1 Auto is not allowed from a level above 2 AU. 77 WARNING Autozero Out of range 2 Check buffers. 3 Clean UV cell. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 104 2 Call for service. 1 Factory calibration for conductivity is lost. The 89 Electrical error monitor can still be used but may not meet speci- Call for service fications for conductivity measurement. 2 Call for service. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 105 The difference between the zero level and full scale 97 WARNING pH scale must be at least 1 pH unit. Increase the span between (0-100%)<1pH unit zero and full scale settings. See Section 5.4 Analog outputs settings, on page 56. Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 106 3 Switch on the module. ERROR key (Esc) ERROR key (OK+Esc) 1 Switch off the module. ERROR number 100 2 Check all connections. 3 Switch on the module. ERROR number 109-113 ERROR number 120-121 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 107 3 Switch on the module. ERROR number 118 1 Switch off the module. Exc x/y in ab.c 2 Check all connections. 3 Switch on the module. Exc DIV/O in ab.c Exc instr in ab.c Exc address in ab.c Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 108 8 Reference information Reference information About this chapter This chapter provides reference information that may become useful when installing, operating, maintaining and troubleshooting Monitor UPC-900. In this chapter This chapter contains the following sections: Section See page 8.1 Specifications 8.2 Menu overview 8.3 Ordering information...
  • Page 109 Static noise short term 40×10–6 AU at 254 nm (typically 6×10–6 AU at 254 nm) long term 40×10–6 AU at 254 nm Static drift ±100×10–6 AU/h at 254 nm Flow sensitivity 2×10–4 AU min/ml Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 110 Flow rate sensitivity ±1% within 0 to 100 ml/min Conductivity flow cell Property Value Flow rate 0 to 100 ml/min Maximum pressure 5 MPa (50 bar, 725 psi) Generated backpressure Max. 0.01 MPa at 100 ml/min Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 111 Maximum deviation 0.1 pH units pH flow cell Property Value Flow rate 0.1 to 100 ml/min Maximum pressure 0.5 MPa (5 bar, 72 psi) Generated backpressure Maximum 0.02 MPa (0.2 bar, 2.9 psi) at 100 ml/min Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 112 UV flow cells Quartz, ETFE (ethylene tetrafluoroethylene), titani- conductivity flow cell Titanium, CTFE (chlorotrifluoroethylene) pH electrode Glass, FFKM (perfluororubber) flow cell Titanium dummy electrode PTFE (polytetrafluoroethylene) pH stability range 1 to13 (1 to14, <1 day exposure) Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 113 Zn, 214 nm, room temperature 2000 hours Chemical resistance The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatogra- phy of biomolecules, except 100% ethylacetate, 100% hexane, and 100 % tetrahydrofuran (THF). Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 114 Date of Maintenance: Buzzer Test See next page for sub menus Setup Setup Setup Language Set Clock Setup Unit Number Set Timer Setup Display Angle (18:34:52) 00.00.00 Set Alarm 00:32:21 Alarm/Timer 12:30:52 Alarm/Timer off? Buzzer 08:23:34 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 115 Set Averaging UV sub menus (1.3S) 0.64 Set Averaging (1.3S) Setup Lamp Run Time Setup Lamp Run Time Hg (2000h) 3000 Hg (2000h) Setup Show UV (on) Setup Show UV (on) on off Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 116 18-1111-26 pH flow cell, round tip, including 18-1112-92 dummy electrode Dummy electrode, round tip 18-1111-03 pH electrode complete, round tip, 18-1134-84 incl. pH electrode, flow cell and holder Flow cell holder UPC-900 18-3055-87 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 117 Tubing cutter 18-1112-46 U-wrench, M6 19-7481-01 U-wrench, 1/4" 18-1112-45 Signal cable, 6 pin mini-DIN-open 18-1110-64 Chart Recorder REC 111, 1 channel 18-1132-32 Chart Recorder REC 112, 2 channel 18-1132-33 A = accessories C = consumables Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 118 Bio-Sciences Co rp, 800 Centennial A Avenue, P.O. Box 132 7, Piscataway, NJ 08855-1 1327 © 2010-14 General Electric Com mpany—All rights res served. First publishe ed April 2010. DOC1149542/28-980 00-26 AC 05/2014 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 119 7, Piscataway, please e call local tec chnical suppo rt or custome service. NJ 08855-1 1327, US © 2010-14 General Electric Com mpany—All rights res served. First publishe ed April 2010. DOC1149544/28-980 00-27 AC 05/2014 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 120 Change, 94 Setup, 80 Storage, 95 Conductivity measurement Troubleshooting, 100 Maintenance, 85 Conductivity temperature Manufacturing information, 7 Setup compensation, 72 Monitor Setup reference tempera- Clean, 86 ture, 73 Notes and tips, 7 Decontamination, 19 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
  • Page 121 UV flow cell, 26, 34 Clean, 87 Storage, 94 Safety UV lamp notices, 6 Control, 54 Safety precautions, 12 UV measurement Service display, 83 Troubleshooting, 97 Service mode UV signal Check, 69 Filter noise, 64 Site requirements, 32 Monitor UPC-900 Operating Instructions 29-0567-16 AB...
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  • Page 124 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare repre- sentative for the most current information.