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Symptoms
Power failure occurs during the instrument
run.
Update of firmware from the SD Card is
not successful.
Need to cycle power on the instrument.
10.Appendix
A. User-Defined Methods
The Maxwell
®
16 MDx Instrument allows the generation
and use of customized user-defined methods. You can
generate a method that optimizes purification from
challenging sample types or unique applications.
User-defined sample processing times for lysis, binding,
drying and elution can be set. In addition, the number of
cycles can be customized for particular samples. The new
run parameters can be saved on the instrument as a user
method. The actual effect of any change must be determined
through user testing. The potential benefits and
disadvantages of changing any of these parameters are
listed below. The user will need to optimize parameters.
Potential Effects of Changing Run Parameters
Changing Lysis Time: This is the amount of time that the
plunger mixes the sample before addition of MagneSil
particles to the sample.
•
Potential Benefits: Complete lysis of the sample is
necessary to recover DNA, RNA or protein. The optimal
lysis time for a specific sample type depends on the
time required to adequately lyse the samples. Some
samples will not require additional lysis time after
preprocessing, while other samples will benefit from
an extended lysis time.
•
Potential Disavdantages: Extended lysis times could result
in the formation of precipitates, which may interfere
with purification and downstream applications.
Promega Corporation
2800 Woods Hollow Road • Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 • Phone 608-274-4330
Maxwell
®
16 MDx Instrument Technical Manual
Causes and Comments
To recover your samples after a power failure, first ensure that the
particles are in one of the wells of the cartridge and are not attached
to the plunger. If the power failure occurred at a point where the
magnetic particles were captured on the outside of the plungers,
manually move the plungers up and down in the wells to wash the
particles off.
Switch the instrument on. Note the UV light will not come on during
the self-diagnostic checks that the instrument performs. However, if
you have scheduled the UV lamp to come on after instrument
initialization, cancel the UV treatment to avoid losing the sample. In
the Home screen restart the run from the beginning. Add new plungers
during run set up.
Note the power outage will prevent the UV light coming on during
initialization, and it will also cancel any programmed UV treatment
until a successful run has been completed. Cycle the power after a run
has been successfully completed to re-initialize the UV system.
To update the firmware you must use the supplied 1Gb SD card. Other
1Gb SD cards may work, but Promega does not support other SD
cards. It is recommended that you leave the SD card in its slot when
not in use to prevent losing it.
If you need to recycle the power, it is extremely important to remove
any samples from the instrument before cycling the power on the
instrument because the UV lamp comes on as part of the startup
diagnostic tests.
Changing Binding Time: This is the amount of time that
is allowed for binding the MagneSil
the nucleic acid or protein.
•
•
Changing Drying Time: This is the amount of time prior
to elution that the particles are air-dried.
•
®
•
Changing Elution Time: This is the amount of time that
the plunger is used to mix particles in the elution buffer.
•
Potential Benefits: Maximal recovery requires efficient
binding of the target molecules to the magnetic
particles. Increased binding time can increase binding
of the target molecule
Potential Disadvantages: Increased binding times may
increase carryover of detergents, contaminants and
other components that may affect purity or inhibit
downstream applications.
Potential Benefits: Excess alcohol from the wash buffers
can interfere with downstream applications such as gel
electrophoresis, PCR and enzymatic reactions.
Increasing the drying time can remove excess alcohol
from the target.
Potential Disadvantages: Overdrying can reduce recovery
of the target molecules from the MagneSil
Potential Benefits: Efficient recovery of the target
molecules requires removal from the MagneSil
particles. Extending the elution time can enhance the
removal of the target molecules from the MagneSil
particles.
®
particles containing
®
particles.
®
®
part# TM320
rev. 9/09
24
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