Homogenization Method; Promega Corporation - Promega Maxwell 16 MDx Technical Manual

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Figure 48. User Protocol screen.
2. In the User Protocol screen, select View to see the
parameters for an existing method. Select Edit, and
enter the correct PIN if the method is PIN-protected.
Figure 49. Edit Protocol.
3. Selecting Edit Protocol opens a screen that displays the
current method name on the left of the title bar and the
current method type on the right. To change the
purification method type (e.g., from DNA to RNA),
select the method listed in the screen header. This will
open the Choose a Method screen where you can
choose DNA, RNA or Protein (SEV) or DNA, RNA or
Total NA (LEV). Select the parameter to be changed,
and change as described above.

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Maxwell
®
16 MDx Instrument Technical Manual
4. To change a custom method name or PIN in the User
5. To change the protocol name, select Edit/Add Protocol
B. Homogenization Method
Both the SEV and LEV modes have an option that allows
homogenization of tissue samples prior to running the
purification method. These methods must be user-validated
Figure 50. Edit run parameters.
Protocols screen, select the existing custom protocol
name to be changed, and press the Run/Stop button.
In the next User Protocols screen, select Edit. If the
method is PIN-protected, enter the PIN. In the next
User Protocol screen select Administration to open the
User Protocol Options screen.
Figure 51. User Protocol Options.
Name; use the keyboard to enter the new name.
To change a protocol PIN, select Edit/Add PIN, and use
the keypad screen to add and confirm the new PIN.
To remove the protocol name or PIN, choose Remove
Protocol Name or Remove PIN. After confirming that you
wish to remove a name or PIN, the instrument will
remove these settings.
The user-defined methods may be run by selecting User
in the Protocols screen (see Section 5.A).
part# TM320
27
rev. 9/09

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