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Figure 21. Maxwell
16 Configuration screen. The functions to
change the hardware (Hardware) and firmware (Configuration)
settings (see Section 7) are accessible from this screen. Also, the
UV lamp may be run from this screen (UV On; Section 8). This
screen also provides access to the Service functions (Service Mode;
Section 10.D). User protocols also may be set up from this screen
by selecting User Protocol (Section 10.A).
5. Operating the Maxwell
A. Preprogrammed Methods
The preprogrammed methods supplied can be used to
purify DNA, RNA and Protein, and the options available
differ between the SEV and LEV mode. The protocol to be
used can be chosen during run setup.
Setting up a Run in the SEV Mode
1. The Home screen must indicate SEV. Select Run. Select
the User, and add the matching PIN if appropriate.
Pressing the Run/Stop button opens the Protocols
screen. From this screen, you can select a protocol.
Figure 22. Protocols screen.
Promega Corporation
2800 Woods Hollow Road • Madison, WI 53711-5399 USA
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Maxwell
®
16 MDx Instrument
®
16 MDx Instrument Technical Manual
2.
•
Select DNA to access the protocols for Blood and
Cells (use with the Maxwell
Purification Kit Promega Cat.# AS1010 or Maxwell
16 Cell DNA Purification Kit, Cat.# AS1020), Buffy
Coat (use with the Maxwell
Purification Kit Promega Cat.# AS1010) or Tissue
DNA (use with the Maxwell
Purification Kit Promega Cat.# AS1030) purification.
Select the method required.
•
Select RNA to access the protocol for Blood, Cells
and Tissue RNA (use with the Maxwell
RNA Purification Kit, Promega Cat.# AS1050)
purification.
•
Select Protein to purify polyhistidine-tagged protein
from cells (use with the Maxwell
Protein Purification Kit, Promega Cat.# AS1060).
•
Select User to access and run custom protocols.
Instructions for setting up customized protocols are
presented in Section 10.A. Select the method
required.
•
Select Other to access specialized protocols for
Homogenization or Fixed Yield DNA and to access a
Demo option.
Homogenization allows limited homogenization of
some types of tissue samples prior to running the
purification method. See Section 10.B.
Fixed Yield DNA purifies a limited amount of DNA,
allowing a reproducible amount of DNA to be
captured between samples.
The Demo protocol is for demonstration purposes
only and should not be used for sample purification.
Please contact Promega Technical Services at
techserv@promega.com for more information on
these specialized options.
3. A confirmation screen allows you to verify that the
correct selection has been made. If correct, press the
Run/Stop button, and you will be prompted to open
the door to load the samples. Press the Run/Stop button.
Open the door. The cartridge rack will extend, ready
for sample loading. Follow the steps in Section 5.B and
5.C to gather the run data and perform the run. Follow
the steps in Section 6 to generate the run reports.
Important. At this point there are different paths
!
forward, depending on whether the run data reporting
options are being used. See Section 5.B for more
information.
Setting Up a Run in the LEV Mode
1. The Home screen must indicate LEV. Select Run. Select
the User, and add the matching PIN if appropriate.
Pressing the Run/Stop button opens the Protocols
screen. From this screen, you can select a protocol.
®
16 Blood DNA
®
®
16 Blood DNA
®
16 Tissue DNA
®
16 Total
®
16 Polyhistidine
part# TM320
rev. 9/09
11
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