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Summary of Contents for Labnet MultiGene OptiMax Thermal Cycler
- Page 1 Labnet MultiGene™ OptiMax Thermal Cycler Instruction Manual Catalog Numbers: TC9610 TC9610-230V...
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Page 2: Table Of Contents
Table of Contents 9.0 Creating a New Protocol ......11 1.0 Introduction .........1 9.1 Changing Temperature/Time Values . -
Page 3: Introduction
1.0 Introduction The Labnet MultiGene™ OptiMax is a powerful, reliable, and affordable thermal cycler. It is equipped with a gradient function that enables the user to set a maximum 24°C temperature gradient between the BLOCK #1 to BLOCK #6 (16-well/block). -
Page 4: Specifications
Pollution Degree 2 5.0 Controls The front panel of the Labnet MultiGene OptiMax thermal cycler includes a display panel and keypad which consists of function keys (F1 to F5), alphanumeric keys, directional arrow keys and the ENTER key. Alphanumeric... - Page 5 Lid knob Sample block Keypad Control panel Air vents (left and right sides) Air vents (front) Figure 2. Front view. Power cable connector USB port Brightness Power switch adjustment knob Product label RS-232 port Air vents (rear) Figure 3. Rear view. Instruction Manual...
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Page 6: Getting Started
For run summary download to computer. 6.0 Getting Started 6.1 Unpacking Open the Labnet MultiGene™ OptiMax thermal cycler package, and confirm that all items listed below are included: Labnet MultiGene OptiMax thermal cycler unit Electric fuses (7A and 3.15A) ... -
Page 7: Lid Opening/Closing
6.4 Lid Opening/Closing There are two ways to open the lid: 1) Slide the lid to the rear. This method is recommended for common operations and to prevent the skin burning from the heated lid. 2) Lift up the lid. This method is used to clean up the heating plate of the lid. Rotate the lid knob clockwise to lower down the heating plate and press down on the cap of the tubes, tighten until you feel resistance. -
Page 8: Function Map
User User Data Log EDIT DELETE (VIEW) Protocol Protocol Protocol Date & Time Protocol Lid Temp Beeper Insert Administrator Delete Service Mode Advanced Ramping Speed Optimization Tm Calculator Increment Pause Save Figure 9. Operation flowchart. LABNET MULTIGENE OPTIMAX THERMAL CYCLER... -
Page 9: Key Entry System
6.7 Loading the Reaction Vessel For the optimal performance of the MultiGene thermal cycler, we recommend Labnet tubes and plates. The recommended sample volume for 0.2 mL tubes or 96-well microplates is 5-100 µL. If using the heated lid with full skirted 96-well microplates it is highly recommended to use a silicone compression mat. -
Page 10: Viewing A User Folder
PIN, and press F1:ACCEPT or ENTER. The screen will prompt “Do you want to delete”. Press F1:ACCEPT to confirm the deletion. Press F5:EXIT to abort the deletion. NOTE: User folders which contain any protocols can’t be deleted except by the administrator. LABNET MULTIGENE OPTIMAX THERMAL CYCLER... -
Page 11: Protocol Management
8.0 Protocol Management After entering the selected User folder, users can press F1-F4 keys to Run, New (Create), Edit (View), and Delete a selected Protocol as shown below. 8/21/2022 11:00 Mike>PROTOCOL PROTOCOL DATE TIME Touchdown 07/25/11 09:10:20 07/28/11 03:25:30 Please select a protocol and press EDIT DELETE EXIT... -
Page 12: Pause A Protocol
4. The display screen will return to Protocol screen. Mike>Lab>RUN>START 8/21/2022 11:00 1 Tmp 3 Tmp 25 Cyc 2 Tmp 1 Cyc 94.0 94.0 72.00 4.00 Temp Time 10:00 5:00 0:30 Estimated Remaining: Do you want to abort? LABNET MULTIGENE OPTIMAX THERMAL CYCLER... -
Page 13: Creating A New Protocol
9.0 Creating a New Protocol A new Protocol can be created by going to the User folder and selecting and editing a basic Protocol format, and then assigning it a new name. 1. Select User name on the User screen. Press F1:OPEN or ENTER. 2. -
Page 14: Advanced Settings
To remove the Optimization Temperature setting, place your cursor in the same temperature segment with “G” Gradient Temperature symbol. Press F2:OPTI again. The screen will show each BLOCK temperature settings. Press F1:OFF to clear the Optimization Temperature setting. Press F5:EXIT will delete the Gradient Temperature setting and its symbol. LABNET MULTIGENE OPTIMAX THERMAL CYCLER... - Page 15 Optimization temperature ranges Temperature setting range: 30.0°C to 99.0°C Temperature difference: 0.1°C to 24.0°C BLOCK #1 BLOCK #2 BLOCK #3 BLOCK #4 BLOCK #5 BLOCK #6 Figure 10. Block layout of 96-well model. 8/21/2022 11:00 Mike>PROTOCOL>NEW>OPTI Block 50.0 58.0 54.0 Block...
- Page 16 Only Multi-cycle segments will have incremental/decremental time or temperature function. BBB>PROTOCOL>NEW>INCRE. 8/21/2022 13:00 1 Tmp 2 Tmp 3 Tmp 25 Cyc Temp 94.0 94.0 55.0 72.0 0:30 Time 5:00 0:30 0:30 10:00 Temperature Increment: + 0.0 EXIT CLEAR LABNET MULTIGENE OPTIMAX THERMAL CYCLER...
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Page 17: Saving A Protocol
Time Increment BBB>NEW>INCRE. 8/21/2022 13:00 1 Tmp 2 Tmp 3 Tmp 25 Cyc Temp 94.0 94.0 55.0 72.0 Time 0:30 10:00 5:00 0:30 0:30 Time Increment: + 0:00 CLEAR EXIT 9.4.3 Pause Setting Position your cursor on the desired temperature segment. Press F4:PAUSE to add a pause action during operation. Pause will appear on the Temperature Segment graph. -
Page 18: Deleting A Protocol
NOTE: Protocols in the Preset Protocols Folder can’t be deleted, while Protocols in the Public Folder can be deleted by any user if not password protected. MIKE>PROTOCOL 8/21/2022 11:00 PROTOCOL DATE TIME Touchdown 07/25/11 09:10:20 07/28/11 03:25:30 Are you sure you want to delete? LABNET MULTIGENE OPTIMAX THERMAL CYCLER... -
Page 19: System Setup
12.0 System Setup System Setup contains 8 functions: 1. Data Log: Contain the history of the latest-run Protocol. 2. Date & Time: Change the Date and Time settings. 3. Lid Temperature: Choose the preferred lid temperature as default setting during running a Protocol. 4. -
Page 20: Lid Temperature
Users can turn ON or OFF the sound of this unit by selecting the Beeper screen under SYSTEM screen. NOTE: Remember to press F1:ACCEPT to store your settings. SYSTEM>BEEPER 8/21/2022 11:00 Beeper Function: (1:ON 2:OFF) Press F1 to save your settings EXIT ACCEPT LABNET MULTIGENE OPTIMAX THERMAL CYCLER... -
Page 21: Administrator
12.4 Administrator The password of Administrator is “9610”. The Administrator of this unit has rights to delete any User Folder and any Protocols inside a User’s Folder. 12.5 Service Mode Only authorized service personnel have the password to enter Service Mode and perform necessary maintenance and repairs. 12.6 Ramping Speed Users can choose the Ramping Speed. -
Page 22: Maintenance And Troubleshooting
13.2 Cleaning the Heated Lid Make sure the Labnet MultiGene thermal cycler is unplugged and cooled down. To clean the heated lid, slide the lid back on the tracks until the front black pins align with the two slots, taking care that the Rolleiflex cable is clear. Lift the lid from the front, (be careful of sharp edges) and flip up. -
Page 23: Troubleshooting
14.0 Troubleshooting Problem Cause Solution The display is Off even when power is Power is not reaching the system. Check power source voltage. switched On. Power cord is not plugged into the socket Reconnect the power cord. properly. Power fuse is blown out. Replace the power fuse. -
Page 24: Limited Warranty
(WEEE), this product is marked with the crossed-out wheeled bin and must not be disposed of with domestic waste. Consequently, the buyer shall follow the instructions for reuse and recycling of waste electronic and electrical equipment (WEEE) provided with the products and available at www.corning.com/weee. LABNET MULTIGENE OPTIMAX THERMAL CYCLER... -
Page 25: Appendix: Protocols In Default Folder
Appendix: Protocols in Default Folder 2-Step PRESET>2-STEP>RUN 8/21/2011 11:00 2 Tmp 25 Cyc 2 Tmp 1 Cyc 1 Tmp Temp 94.0 94.0 55.0 55.00 4.00 Time 10:00 5:00 0:30 0:30 Mode:1(1-2) Volume: 50ul(5-100ul) 1:Tube Control 2:Block Control START EXIT Gradient ... - Page 26 START Time Increment PRESET>Time inc.>RUN 2011/8/21 13:00 3 Tmp 20 Cyc 2 Tmp 1 Tmp - 0:05 95.0 95.0 65.0 72.0 5:00 0:30 0:30 10:00 Mode:1(1-2) Volume: 50ul(5-100ul) 1:Tube Control 2:Block Control START EXIT LABNET MULTIGENE OPTIMAX THERMAL CYCLER...
- Page 27 To request certificates, please contact us at www.labnetlink.com. Warranty/Disclaimer: Unless otherwise specified, all products are for research use or general laboratory use only.* Not intended for use in diagnostic or therapeutic procedures. Not for use in humans. These products are not intended to mitigate the presence of microorganisms on surfaces or in the environment, where such organisms can be deleterious to humans or the environment.
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