Related Manuals for Labnet MultiGene OptiMax TC9610
Summary of Contents for Labnet MultiGene OptiMax TC9610
- Page 1 MultiGene OptiMax Thermal Cycler TC9610 /TC9610-230 Operation Manual Ver.2.1 Midwest Scientific, Inc.
- Page 2 Notice This instrument is licensed for research and development and for uses other than human in vitro diagnostics. Notwithstanding explicitly licensed patents, no right is conveyed expressly, by implication or by estoppel under any patent owned or controlled by Life Technologies Corporation, including, but not limited to, patents that cover thermal cyclers capable of real-time detection.
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Page 3: Table Of Contents
Table of Contents SAFETY PRECAUTIONS ....................1 GENERAL DESCRIPTION .....................2 2.1 F ........................2 EATURES 2.2 P ....................... 2 RODUCT VERVIEW GETTING STARTED......................6 3.1 U ........................6 NPACKING 3.2 I ......................6 NITIAL PERATION 3.3 H ........................6 EATED 3.4 L ....................7 PENING LOSING 3.5 F ......................10 UNCTION... - Page 4 9.1 D ........................26 9.2 D & T ........................27 9.3 L ......................27 EMPERATURE 9.4 B .........................28 EEPER 9.5 A ......................28 DMINISTRATOR 9.6 S ......................28 ERVICE 9.7 R ......................28 AMPING PEED 9.8 T ......................29 ALCULATOR MAINTENANCE AND TROUBLESHOOTING ............30 10.1 C ....................30 LEANING THE UNIT 10.2 C...
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Page 5: Safety Precautions
MultiGene Thermal Cycler Safety Precautions Before using the MultiGene OptiMax thermal cycler for the first time, please read this entire operating manual carefully. To guarantee problem free, safe operation of the MultiGene OptiMax thermal cycler, it is essential to observe the following points. Do not use the machine in a potentially explosive environment or with potentially explosive chemicals. -
Page 6: General Description
General Description The MultiGene OptiMax thermal cycler is a powerful, reliable and affordable thermal cycler. It is equipped with a gradient function that enables the user to set a maximum 24 °C temperature gradient between the BLOCK＃1 to BLOCK＃6 (16 well/block). 2.1 Features Easy-to-operate slide and lever type lid which prevents accidental skin burn ... - Page 7 MultiGene Thermal Cycler Figure 1. Front Panel Overview Name Function Function Keys Use the function keys (F1 to F5) to activate the “button” displayed on the screen above the key; for example, F1 for RUN. (F1 to F5) The four Directional Arrow keys can move the cursor into different Directional directions.
- Page 8 Figure 2. Front View Name Function The lid with heating pad is designed to protect against evaporation resulting from heating of the reaction tube and reaction plate. Rotate Lid Knob in clockwise will lower the heating plate to place Lid Knob pressure on the cap of tubes.
- Page 9 MultiGene Thermal Cycler Figure 3. Rear View Name Function Power Cable Power cable socket and fuse. Connector Power On/Off switch Power Switch Control the brightness of LCD panel. Please use Philip-head screw Brightness driver to adjust it. Adjustment Potentiometer For run summary download to computer USB Port For run summary download to computer RS-232 Port...
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Page 10: Getting Started
The top also places pressure on the caps of tubes and film on titer plates to prevent vapor loss and cross contamination of samples. When using plates it is highly recommended to use a Silicone Compression Mat (Labnet TC96-CM-10, for 96-well models), to assist in placing even pressure on the plates. -
Page 11: Lid Opening/Closing
MultiGene Thermal Cycler 2. To avoid heat dissipation, the Heated Lid should be closed and tighten during a protocol run. Keeping the heated lid open during a protocol run might cause “HEATER ERROR”, because the heated lid fails to reach the set temperature. 3. - Page 12 Figure 5. Slide the lid backward to access the block Figure 6. Slide the lid to align the lid hinge with notches of top case Figure 7. Lift the lid...
- Page 13 MultiGene Thermal Cycler Figure 8. Rest the lid in vertical position...
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Page 14: Function Map
3.5 Function Map The User Screen includes 5 function options; 1) VIEW folders under selected User folder, 2) NEW(Create) a User, 3)EDIT a User, 4)DELETE a User, 5)SYSTEM. Once a user folder is selected and the password is approved, users can; 1) RUN a Protocol, 2)NEW(Create) a protocol, 3) EDIT(VIEW) a Protocol, and 4) DELETE a Protocol. -
Page 15: Key Entry System
If using the heated lid with 96 well plates it is highly recommended to use a Silicone Compression Mat ( Labnet TC96-CM-10, Axygen CM-96-RD ) to assist in placing even pressure on the plates. Note: To avoid deformation of the sample tubes or Strips, it’s important to load at least one tube in each of the four corners and at least four tubes in the center of the block, this will distribute the pressure evenly throughout the surface. -
Page 16: User Folder Management
User Folder Management After the unit is turned on, the initial screen which contains software version and manufacturer’s name will show up for 10 seconds and then enter User Folder screen. Pressing any key will go to User Folder screen immediately. A sample screen is shown below. -
Page 17: Creating A New User Folder
MultiGene Thermal Cycler Identification Number(PIN) as shown in the screen below. After the PIN is entered, press F1:ACCEPT or ENTER key to confirm the PIN. To run a Protocol, please refer Section 5.1. > > 4.2 Creating a New User Folder Under User Folder screen, press F2:NEW can create a new User folder. - Page 18 selected User folder. You are required to enter the PIN and press F1:ACCEPT or ENTER . The screen will prompt “Do you want to delete”. Press F1:ACCEPT to confirm the deletion. Press F5:EXIT to abort the deletion. Note: User folders which contain any protocols can’t be deleted except by administrator.
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Page 19: Protocol Management
MultiGene Thermal Cycler Protocol Management After entering the selected User Folder, users can press F1~F4 keys to Run, New(Create), Edit(View) and Delete a selected Protocol as shown below. > > 5.1 Running a Protocol 1. Press F1:RUN to run the selected Protocol under the User folder screen. The screen will show as below. -
Page 20: Pause A Protocol
2. Users are required to select a Temperature Control Mode before running the Protocol. Mode 1(Tube Control): Enable system operation with simulated temperature control. If Mode 1 is selected, users are required to input the sample volume : 5-100ul. In this mode, temperature control is based on Block temperature and an algorithm which estimates sample temperature based on the block temperature and the sample volume. -
Page 21: Terminating A Protocol
MultiGene Thermal Cycler > > > > 94.0 94.0 55.0 72.0 72.00 4.00 ∞ ∞ 5.4 Terminating a Protocol 1. Press F5:EXIT or STOP key in the bottom-left side of the Alphanumeric keypad to terminate a running protocol. 2. The display will show message “Do you want to abort?” as below. 3. -
Page 22: Creating A New Protocol
Creating a New Protocol A new Protocol can be created by going to the User Folder and selecting and editing a basic Protocol format, and then assigning it a new name. 1. Select User name on User screen. Press F1:OPEN or ENTER button to enter User Folder. -
Page 23: Inserting/Deleting Temperature Segment
MultiGene Thermal Cycler The temperature and time ranges are shown below. ◦ ◦ Temperature range: 4.0 to 99.9 Time range (minute: second): 00:01 to 99:59 ( 99:99 = ∞ ) 6.2 Inserting/Deleting Temperature Segment Temperature Segment can be inserted or deleted by selecting F2:INSERT or F3:DELETE key. - Page 24 > > > > > > ∞ ∞ 6.4.1 OptimizationTemperature Setting 1. To enter an Optimization Temperature setting, place your cursor on the desired Temperature Segment. Select F2:OPTI. to enter the screen below. 2. The temperatures of all BLOCK offset are based on BLOCK＃1. If press F2:AUTO ◦...
- Page 25 MultiGene Thermal Cycler BLOCK＃1 BLOCK＃2 BLOCK＃3 BLOCK＃4 BLOCK＃5 BLOCK＃6 Figure 10. Block layout of 96-well model The Optimization temperature ranges are shown below. ◦ Temperature setting range: 30.0 to 99.0 ◦ Temperature difference: 0.1 to 24.0 > > > > >...
- Page 26 6.4.2 Incremental/Decremental Temperature and Time Settings To increase or decrease the dwell time or temperature after each repeat you must have more than one cycle repeat. i.e. 55ºC and a dwell time 40 seconds, you could decrement the second step by 5 seconds the second step would be 55ºC and a dwell time of 35 seconds.
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Page 27: Saving A Protocol
MultiGene Thermal Cycler Note: Temperature limits 4.0ºC to 99.9ºC. DO NOT exceed. Only Multi-cycle segments will have incremental/ decremental time or temperature function. Time Increment > > > > ∞ ∞ 6.4.3 Pause Setting Position your cursor on desired temperature segment. Press F4:PAUSE to add a pause action during operation. -
Page 28: Editing(Viewing) A Protocol
Editing(Viewing) a Protocol An editing Protocol can be saved under a new name (newly created Protocol) or under the same name (edited Protocol). Under the User’s Folder, choose the Protocol to be edited. Select F3:EDIT to edit the selected Protocol. This displays the existing Protocol and enables editing of the temperature segment and numerical values as shown below. -
Page 29: System Setup
MultiGene Thermal Cycler > > Note: Protocols in the Preset Protocols Folder can’t be deleted, while Protocols in the Public Folder can be deleted by any user if not password protected. System Setup System Setup contains 6 functions: Data Log: contains the history of the latest-run Protocol. Date &... -
Page 30: Data Log
& & 9.1 Data Log The Data Log records a log of activity for the most recently run Protocol. Executing a new Protocol updates the history log by overwriting previous log. To export log to a PC a Hyper Terminal communication protocol must... -
Page 31: Date & Time
MultiGene Thermal Cycler 9.2 Date & Time Users can change the date type, default date and time of this unit by selecting Date &Time screen as shown below under SYSTEM screen. Please remember to press F1:ACCEPT to store your settings. >... -
Page 32: Beeper
9.4 Beeper Users can turn ON or OFF the sound of this unit by selecting the Beeper screen as shown below under SYSTEM screen. Please remember to press F1:ACCEPT to store your settings. > > 9.5 Administrator The password of Administrator is “9610”. The Administrator of this unit has rights to delete any User Folder and any Protocols inside a User’s Folder. -
Page 33: T Mcalculator
MultiGene Thermal Cycler > > 9.8 Tm Calculator Users can press the number of the bases of A, T, C and G in the sequence, then press F1:RUN to get the Tm value of the sequence. > >... -
Page 34: Maintenance And Troubleshooting
Maintenance and Troubleshooting 10.1 Cleaning the unit Please ensure that no liquid is spilled onto or into the unit. In addition, periodically wipe it clean of dust and other residue that comes with normal operation of the unit. Use a soft, lint-free cloth and deionized water. -
Page 35: Troubleshooting
MultiGene Thermal Cycler the unit. Slide the new block into place and connect the plug. Gently slide the block until the two screw holes are lined up. Replace the two screws and plugs. Shut the face panel and secure it with two screws. Troubleshooting Problem Cause... -
Page 36: Error Messages
2. Faulty LCD panel 2. Return unit service. Display is too dark or light Backlight brightness is not Adjust Display Brightness adjusted properly. Potentiometer. 1. Foreign matter, between Lid will not close. Remove the foreign object or lid heater and sample matter. -
Page 37: Appendix A: Technical Specifications
MultiGene Thermal Cycler Appendix A: Technical specifications Model Name TC9610/TC9610-230V Sample Capacity 0.2ml x 96 tubes 4 ~ 99.9 °C Temperature Ranges +/- 0.5°C ( 30 to 99.9 °C) Temperature Accuracy Temperature Uniformity +/- 0.5°C 4°C / 3 °C Max. Temp. Increment/Decrement Rate 30 ~ 99 °C (Max. -
Page 38: Appendix B: Protocols In Default Folder
Appendix B: Protocols in Default Folder 2-Step > > > > ∞ ∞ Gradient > > > > ∞ ∞... - Page 39 MultiGene Thermal Cycler Touch down > > > > ∞ ∞ Time Increment > > > > ∞ ∞...
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Page 40: Appendix C: Ce Declaration
Appendix C: CE Declaration Labnet International Inc. 31 Mayfield Avenue Edison, NJ 08837, U.S.A. Declaration of Conformity Product Name: MultiGene OptiMax Thermal Cycler Model Names: TC9610 /TC9610-230 All models comply with the following European standards: EMC: EN 61326 (Group 1, Class B) -
Page 41: Appendix D: Hyper Terminal And Usb Driver Setup
MultiGene Thermal Cycler Appendix D: Hyper Terminal and USB Driver Setup Users are required to purchase the USB or RS-232 cable to connect the MultiGene and the PC. For USB connection, users are required to install the USB driver. Hyper Terminal Setup: Click on the start tab of Windows and go to accessories. -
Page 42: Appendix E: Multigene Recommended Consumables
Compression Mat TC96-CM-10 Silicone Compression Mat Sealing Tape Clear sealing tape will seal 96 and 384 well Plates when used with Labnet compression mat this polypropylene film eliminates well to well contamination and cross-over in PCR applications Functional Temperature range -40ºC to +104ºC... - Page 43 MultiGene Thermal Cycler compression mat this polypropylene film eliminates well to well contamination and cross-over in amplification applications. Ideal for light sensitive samples and its uniformly applied adhesive provides optimum well sealing Functional Temperature range -80ºC to +104ºC Cat# TC-AS-100. Silicone Compression Mat is used to seal 96 well plates when using the MultiGene Thermal Cycler heated lid function.
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