Protocol Management; Running A Protocol - Labnet MultiGene OptiMax Thermal Cycler Instruction Manual

8.0 Protocol Management

After entering the selected User folder, users can press F1-F4 keys to Run, New (Create), Edit (View), and Delete a selected Protocol as
shown below.
Mike>PROTOCOL
Please select a protocol and press

8.1 Running a Protocol

1. Press F1:RUN to run the selected Protocol under the User folder screen. The screen will show as below.
Mike>Lab>RUN
2. Users are required to select a Temperature Control Mode before running the Protocol.
Mode 1 (Tube Control): Enables system operation with simulated temperature control. If Mode 1 is selected, users are required to

input the sample volume: 5-100 µL. In this mode, the temperature control is based on Block temperature and an algorithm which
estimates the sample temperature based on the block temperature and the sample volume. Recommended for more accurate
temperature control.
Mode 2 (Block Control): Enables system operation with maximum temperature control based on the actual Block temperature.

Recommended for normal operation.
3. Press F1:START to run the Protocol.
PROTOCOL
Touchdown
Lab
RUN NEW
F 2
F 1
1 Tmp
3 Tmp 25 Cyc
Temp 94.0 94.0
Time
5:00 0:30
Mode:1(1-2)
1:Tube Control
START
F 2
F 1
8/21/2022
DATE
07/25/11
07/28/11
EDIT DELETE
F 3 F 4
8/21/2022
2 Tmp 1 Cyc
55.0 72. 72.00
0
0:30 10:00
Volume: 50ul(5-100ul)
2:Block Control
F 3 F 4
11:00
TIME
09:10:20
03:25:30
EXIT
F 5
11:00
4.00
EXIT
F 5
Instruction Manual | 9