Activation Keys - Beckman Coulter Optima L Series Instructions For Use Manual

Run Preparation

Activation Keys

4
Press
SAVE
Actual values will reappear in the displays.
To select a program for use:
1
Press
PROG
2
Press the required program number.
3
Press
ENTER/RECALL
To modify a parameter during the run without changing the program, change the setting as
described above and then press
the modified settings. The lighted number above the
instrument is no longer running from program memory.
Activation Keys
Use the activation keys to control the following ultracentrifuge functions.
VACUUM
1
The vacuum system is activated automatically when you press
begin a run.
2
To evacuate the chamber at another time, make sure the chamber door is closed, then press
VACUUM
The chamber door can be opened only when the vacuum has been completely vented and the
rotor is at rest.
When the run begins, the rotor will not accelerate past 3000 rpm until the chamber pressure drops
below 750 microns. At that time, the rotor accelerates to set speed.
As the vacuum system begins to evacuate the chamber, a series of three LEDs (labeled 750, 200, and
<20 microns) blink, remain lighted, or go off to allow you to monitor the change in pressure (see
Table 2.1). When the pressure drops below 20 microns, the < 20 LED will remain lit until
pressed to vent the chamber at the end of the run.
2-8
and the program will be stored in memory under the selected number.
.
and .
START
ENTER/RECALL
.
. Do NOT press . The instrument will run using
SAVE
key will go out to indicate that the
PROG
ENTER/RECALL
and to
START
is
VACUUM
PN LL-IM-12AB