Activation Keys - Beckman Coulter Optima L Series Instruction Manual

Preparative ultracentrifuges
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Run Preparation

ACTIVATION KEYS

[
]
VACUUM
[
]
START
2-8
To select a program for use:
1. Press [
].
PROG
2. Press the required program number.
3. Press [
ENTER/RECALL
To modify a parameter during the run without changing the program,
change the setting as described above and then press [
Do NOT press [
]. The instrument will run using the modified
SAVE
settings. The lighted number above the [
cate that the instrument is no longer running from program memory.
Use the activation keys to control the following ultracentrifuge
functions.
The vacuum system is activated automatically when you press
[
] and [
ENTER/RECALL
START
at another time, make sure the chamber door is closed, then press
[
]. The chamber door can be opened only when the vacuum
VACUUM
has been completely vented and the rotor is at rest.
When the run begins, the rotor will not accelerate past 3000 rpm until
the chamber pressure drops below 750 microns. At that time, the rotor
accelerates to set speed.
As the vacuum system begins to evacuate the chamber, a series of
three LEDs (labeled 750, 200, and <20 microns) blink, remain
lighted, or go off to allow you to monitor the change in pressure (see
Table 2-1). When the pressure drops below 20 microns, the < 20 LED
will remain lit until [
VACUUM
end of the run.
Press [
] and [
ENTER/RECALL
[
], the instrument will not be activated. If this occurs, press
START
[
] and [
ENTER/RECALL
START
] and [
].
START
] key will go out to indi-
PROG
] to begin a run. To evacuate the chamber
] is pressed to vent the chamber at the
] to begin a run. If you press only
START
] again to begin the run.
] .
ENTER/RECALL

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Optima l-100kOptima l-90k

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