Measurements And Data Acquisition - SYSMEX CyFlow Cube 6 V2m Operating Manual

Fault
The result of the Calibration
Beads measurement is not
in the preselected regions of
the original configuration
script

7.1.3 Measurements and data acquisition

Fault
The device emits an
unusual / noisy sound after
starting the measurement
There is no data acquisition
visible (peaks/dots in
histogram/dot plot) during
the RUN phase
CyFlow™ Cube 6 V2m | Operating Manual | February 2020
Remedy
Check within the software if you have loaded the correct
configuration file for your measurement.
Shake the bottle vigorously (e.g. by vortexing) and repeat
the measurement.
Check the date of expiry of the Calibration Beads.
Check the speed settings.
Perform a priming procedure.
Perform a cleaning procedure.
Remedy
Check that the Waste bottle is closed properly and that
the bottle is not cracked.
Check all visible tubing and make sure that they are not
pinched.
Check that liquid (Sheath Fluid and sample) is dropping
into the Waste bottle after starting the measurement.
Check within the software if the laser/s is/are switched
on.
Check if the right trigger parameter has been selected
(choose FSC trigger as default setting, select SSC for
very small cells, e.g. bacteria).
Check gain values and the threshold.
Increase the flow rate.
7 Troubleshooting
33