Start Instrument Operating Software; Priming The Instrument (Initial Cleaning); Multi Laser Measurements - SYSMEX CyFlow Space Operating Manual

The display shows the Windows
5.1.6

Start instrument operating software

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Double click FloMax
After a few seconds, the display shows the FloMax
By confirming with "OK" the CyFlow™ Space is initialized and the last used instrument
settings are loaded.
The display shows empty histograms.
5.1.7

Priming the instrument (initial cleaning)

Use speed 5. Run a tube filled with 1.6 ml Cleaning Solution (REF 04-4009_R) until the
system stops. Remove sample tube and run a second tube with Sheath Fluid (04-4007_R)
for 2 minutes.
The CyFlow™ Space is ready for acquisition.
DANGER
The waste may contain biohazardous material (infectious cells, dyes). To
minimize biohazards, fill about 25 ml of Hypochlorite Solution (REF 04-
4012_R) into the waste container. When emptying the container, be sure
not to come in contact with the fluid. In case of accidental contact, wash
your skin throughout with soap and disinfectant.
5.2

Multi laser Measurements

NOTICE
Alignment of lasers must only be done by Sysmex representatives or by
authorized service personnel.
The CyFlow™ Space Flow Cytometer can be equipped with one up to 4 laser light sources.
Actual optical setup of your instrument may therefore vary. For questions concerning the
laser setup of this instrument, please contact your local Sysmex representative.
In order to minimize crosstalk between multiple light sources, laser beams from different
lasers are focused on different positions (spots) of the flow cuvette. The CyFlow™ Space
Flow Cytometer supports either 2 laser spots (when employing FloMax
laser spots (when employing FloMax
of the CyFlow™ Space is always located on spot 1. Additional lasers are located on spot 2
and spot 3, respectively. Instruments without 488 nm laser, e.g. only with UV-LED or with
532 nm laser will use that light source on spot 1.
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CyFlow Space | Operating Manual | March 2021
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desktop.
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icon to start FloMax
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version 3.x). The blue 488 nm laser as leading laser
CyFlow™ Space operation software.
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Welcome window.
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versions 2.x) or 3
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