THORLABS RSB1 Operation Manual
  1. Manuals
  2. Brands
  3. THORLABS Manuals
  4. Laboratory Equipment
  5. RSB1
  6. Operation manual

THORLABS RSB1 Operation Manual

Raman spectroscopy bundle
Hide thumbs Also See for RSB1:
Table of Contents

Advertisement

Quick Links

Download this manual
Raman Spectroscopy
Bundle
RSB1(/M,) RSBR1(/M),
RSBC1(/M), RSBA1
Operation Manual

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the RSB1 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for THORLABS RSB1

Summary of Contents for THORLABS RSB1

  • Page 1 Raman Spectroscopy Bundle RSB1(/M,) RSBR1(/M), RSBC1(/M), RSBA1 Operation Manual...
  • Page 2 Version: Date: 17-Sep-2021 Copyright © 2021 Thorlabs...

  • Page 3: Table Of Contents

    4.1 Mounting RSB1(/M) 4.2 Mounting the Front End 4.2.1 Mounting RSBR1(/M) 4.2.2 Mounting RSBC1(/M) 4.2.3 Mounting Cage Systems 4.2.4 Mounting on the SM1.5 External Thread of RSB1(/M) 4.3 Laser and Laser Driver 4.3.1 Laser 4.3.1.1 Alternative Excitation Wavelength 4.3.2 Optical Fiber 4.3.3 Laser Driver 4.3.4...
  • Page 4 6.3 Coded-Aperture Raman Spectroscopy 6.4 Parameters Affecting Spectra 7 Maintenance and Service 7.1 Troubleshooting 8 Appendix 8.1 Technical Data 8.1.1 RSB1/M and RSB1 Technical Data 8.1.1.1 RSB1/M and RSB1 Dimensions 8.1.2 RSBR1/M and RSBR1 Technical Data 8.1.2.1 RSBR1/M and RSBR1 Dimensions 8.1.3...
  • Page 5 8.5.2.6 Hiding the Menus and Full Screen Mode 8.5.2.7 Displaying a Split View 8.5.2.8 Displaying a Legend in the Data Display Area 8.5.2.9 Dark Room Mode 8.5.3 Acquiring Data 8.5.3.1 Finding the Spectrometer 8.5.3.2 Selecting the Instrument to Use 8.5.3.3 Acquiring a Single Spectrum 8.5.3.4 Repeated Measurements...
  • Page 6 8.7 Return of Devices 8.8 Manufacturer Address 8.9 Warranty 8.10 Copyright and Exclusion of Liability 8.11 Thorlabs Worldwide Contacts - WEEE Policy...
  • Page 7 Paragraphs preceded by this symbol explain hazards that could damage the instrument and the connected equipment or may cause loss of data. Note This manual also contains "NOTES" and "HINTS" written in this form. Please read this advice carefully! © 2021 Thorlabs...

  • Page 8: General Information

    Raman signal from the sample and focus it onto the RSB1(/M) input aperture. For laser light in- put, the RSBR1(/M) features an FC/PC connector. A sample table is provided to place a speci- men at the optimal focal plane.

  • Page 9: Ordering Codes And Accessories

    For data acquisition and analysis, Thorlabs provides the OSA Software, Raman Edition (Thorlabs OSA Raman Software). The software is shipped with the RSB1(/M) on an USB flash drive which also contains all required drivers. For software updates, please see the chapter...
  • Page 10 Front End for Measurements of Liquid Samples in a Cuvette with the RSBC1/M RSB1/M Base Unit (Metric Type). Included are TR40/M Mounting Post and PH50E/M Post Holder, a CF125C/M Clamping Fork, 3500 ml Quartz Cuvette, and a RPB Polystyrene Calibration Block.

  • Page 11: Requirements

    Chapter 1: General Information 1.2 Requirements These are the requirements for the PC intended to be used for operation of the RSB1(/M) using the Thorlabs OSA Raman Software. Please find the OSA Raman Software on the USB stick provided with the RSB1(/M) main unit or for download from the Raman bundle website.

  • Page 12: Getting Started

    Raman Spectroscopy Bundle mechanically and electrically. Verify that you have received the following items within the package: RSB1 Base Unit (Imperial) RSB1 Main Unit for Raman Spectroscopy USB 3.0 A to Micro B Cable to Connect the Camera to the PC MB1824 Aluminum Breadboard BBH1 Lifting Handles for the Breadboard 2 x RS2.5P Imperial Mounting Posts...
  • Page 13 6. RPB Polystyrene Block for Calibration 7. Quick Reference RSBA1 Adapter Plate for 60 mm Cage Systems 1. 2 RSBA1 Mounting Brackets with 4 Metric Screws (M4) and 4 Imperial Screws (8-32). 2. Quick Reference Page 6 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 14: Operating Elements

    Chapter 3: Operating Elements 3 Operating Elements 3.1 RSB1(/M) Base Unit The RSB1(/M) Base Unit components are labelled in the image below, which shows the RSB1/M model. Aside from the posts and mounting threads, RSB1 and RSB1/M are identical. Attention...

  • Page 15: Rsbr1(/M) Front End For Reflective Measurements

    3.2 RSBR1(/M) Front End for Reflective Measurements The RSBR1(/M) front end components are labelled in the image below, which shows the RSBR1/M model. RSBR1(/M) Features RSB1(/M) Base Unit Input Aperture Optical Baffle Assembly to the RBS1(/M) SM1 Internal Thread SM1 Locking Ring...

  • Page 16: Rsbc1(/M) Front End For Cuvette Measurements

    3.3 RSBC1(/M) Front End for Cuvette Measurements The RSBC1(/M) front end components are labelled in the image below, which shows the RSBC1/M model. RSBC1(/M) Features RSB1(/M) Base Unit Input Aperture Baffle Assembly to the RBS1(/M) SM1 Internal Thread SM1 Locking Ring...

  • Page 17: Rsba1 Mounting Brackets For Cage System

    Raman Spectroscopy Bundle Chapter 3: Operating Elements 3.4 RSBA1 Mounting Brackets for Cage System Page 10 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 18: System Setup

    RSBA1 mounting adapter. In that case, please use the two more distal mounting taps 2 and 3 Attention Be aware that the mounting is less stable when using taps 2 and 3 are used. The RSB1(/M) may fall over. Page 11 MTN022733-D02...
  • Page 19 Mounted RSB1 Mounting RSB1(/M) in an Overhead Position The RSB1(/M) can also be mounted in an up side down position since the assembly is isomet- ric with mounting taps on both sides. Use the mounting taps on the upper side indicated...

  • Page 20: Mounting The Front End

    Weight (£ 0.5 kg) and leverage should not exceed that of RSBR1(/M). Attention Do not mix metric and imperial systems as the post heights vary slightly. Thorlabs does not re- commend to do so as the beam path would need to be carefully adjusted.
  • Page 21 XY, leave three rows of mounting holes between the posts of the RSB1(/M) and the edge of the sample table free, as shown below. The RSBR1(/M) sample table might need to be slightly moved in order to fit the screws into the optical table.
  • Page 22 The focal plane for the sample is 54.1 mm (2.14'') above the breadboard for the metric system RSB1/M and RSBR1/M. For the imperial system RSB1 and RSBR1, the distance is 52.6 mm (2.08'') above the breadboard. Please see the web drawings for additional information.
  • Page 23 Please be aware that the height of the posts differs by about 1.5 mm between metric and im- perial systems. Depending on which post was used to mount the RSB1(/M) Base Unit, this will impact the height of the beam path.

  • Page 24: Mounting Rsbc1(/M)

    For angled positioning of the RSBR1(/M) carefully unscrew the locking ring between the RSB1(/M) Base Unit and the RSBR1(/M) front unit. Carefully turn the RSBR1(/M) on the SM1 thread of the RSB1(/M). Position the locking ring such that the distance between the RSB1(/M) facing internal thread of the output end of RSBR1(/M) and the counter ring equals 16.5 mm...
  • Page 25 ) all the way towards the RSB1(/M) Base Unit. 6. Position the SM1 connecting ring towards the RSB1(/M) Base Unit such that the open end of the baffle assembly is not covered by the connecting ring. 7. Mount the post ) to the main body ) of the RSBC1(/M) front end.

  • Page 26: Mounting Cage Systems

    4.2.3 Mounting Cage Systems The 60 mm cage system can be attached to the RSB1(/M) Base Unit with the help of the RSBA1 adapter. To do so, screw one RSBA1 mounting bracket each to the top and bottom of the RSB1(/M) as shown below to the left, using the supplied screws.

  • Page 27: Mounting On The Sm1.5 External Thread Of Rsb1(/M)

    Please ensure appropriate laser safety measures. 4.2.4 Mounting on the SM1.5 External Thread of RSB1(/M) The SM1.5 external thread on the input aperture end of the RSB1(/M) Base Unit can be used to mount, for example, 1.5'' lens...

  • Page 28: Laser

    Laser Re- quirements 4.3.1.1 Alternative Excitation Wavelength Alternative Excitation Wavelength: Due to the modular design, it is possible to modify the Thorlabs Raman bundle to target the complementary high-wavenumber region from 2800 cm to 3800 cm by choosing an excita- tion wavelength of 680 nm, and changing the filter in the front end.

  • Page 29: Mounting The Laser

    Please see the drawings for CLD1015 online. This ar- mored fiber patch cable is then connected to the FC/PC connector of the Thorlabs front end. Please coil up the fiber pigtail of the laser and store it within the laser compartment of the CLD1015.

  • Page 30: Connecting The Rsb1(/M) To The Pc

    Raman Spectroscopy Bundle Chapter 4: System Setup Note We recommend using a power meter, such as the Thorlabs PM400K2, to check the laser driver setting. 4.4 Connecting the RSB1(/M) to the PC After installing the OSA Raman Software on the PC, connect the camera to the PC using the provided USB 3.0 high-speed cable to a USB 3.0 port of the PC.

  • Page 31: Operating Instructions

    5 Operating Instructions To perform Raman spectroscopy measurements using the RSB1(/M) Base Unit, please as- semble a system consisting of the RSB1(/M) Base Unit, a front end for signal input, a fiber- coupled 785 nm light source that meets the laser requirements , and a laser driver.

  • Page 32: Software And Installation

    " in the Appendix. Software Version Please install the OSA Raman Software provided on the USB data carrier on the PC intended for the use with the RSB1(/M). Please check for updates to the OSA Raman Software on the Thorlabs Raman bundle website: https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=14241...
  • Page 33 Chapter 5: Operating Instructions After the software installation has finished, connect the RSB1(/M) to an available USB 3.0 port on the PC. Please use the cable that is included with the RSB1(/M) Base Unit (Thorlabs Item # USB3-MBA-118 for Scientific Cameras).

  • Page 34: Raman Measurements With The Osa Raman Software

    When first starting the OSA Raman Software, the following screen will appear. Select the "Scan USB" button found under “Sweep” in the main window menu to let the soft- ware automatically recognize the RSB1(/M) base unit. The left panel of the below screen lists the recognized devices.

  • Page 35: Software Configuration Procedure

    Raman Spectroscopy Bundle Chapter 5: Operating Instructions The Raman module is activated once the RSB1(/M) device is selected in the left panel "At- tached Devices". The right panel displays the status information of the connected device and the settings for the selected device.

  • Page 36: Settings

    Raman Spectroscopy Bundle Chapter 5: Operating Instructions The next chapter Settings describes the functionality of the Thorlabs OSA Raman Software settings and how to adjust them. 5.3.3 Settings The Thorlabs OSA Raman Software allows critical parameters for Raman spectroscopy to be set.
  • Page 37 Raman Spectroscopy Bundle Chapter 5: Operating Instructions Open via Devices Icon: Open via Settings Tab: Page 30 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 38: Info Panel

    Raman Spectroscopy Bundle Chapter 5: Operating Instructions 5.3.3.1 Info Panel The "Info" panel displays the status information of the device. Connection: The bullet icon color indicates whether the camera is connected to the PC. Green: The device is connected to PC and recognized by the software. Red: The device is not connected or not recognized by the software.

  • Page 39: Main Panel

    Once loaded, they are stored in the user AppData directory and automatically recog- nized by the Software 2. Adjust the Integration Time and Gain under Acquisition Control 3. Determine and adjust the precise laser wavelength as described in section System Calibra- tion Page 32 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 40 Raman Spectroscopy Bundle Chapter 5: Operating Instructions Page 33 MTN022733-D02...
  • Page 41 The Raman-scattered light passes through the coded matrix with defined slits at the input aper- ture of the RSB1(/M). This leads to exposure of certain pixels in the camera. The Thorlabs OSA Raman Software then calculates the Raman spectrum based on the acquired image and the corresponding information about the position in the coded matrix.
  • Page 42 0 dB. In low light conditions, increasing the analog gain may lead to slight improvements in SNR. The CMOS camera on the RSB1(/M) Base Unit has a maximum analog gain of 24 dB. In- creasing the value up to 48 dB adds digital gain after analog to digital conversion. Please see the chapter "Parameters Affecting Spectra...
  • Page 43 This panel is only visible when opening the Settings dialog via the Settings Tab in the main menu. To deactivate the automatic smoothing of the spectra, select "Do not smooth spectrum". To deactivate the amplitude calibration based on the NIST SRM2241 standard, select "Do not apply amplitude correction". Page 36 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 44: Dark Image

    5.3.4 System Calibration In order to gain meaningful spectra from a Raman spectra using the Thorlabs Raman bundle, a two-step calibration to deliver readings in nanometers with a calibrated amplitude is performed at the factory. A third calibration step to determine the precise excitation laser wavelength must be done by the customer.
  • Page 45 (NIST-SRM-2241) in order for the counts read to correspond to a known number of photons. All calibration data necessary to reconstruct spectra from the measurements are supplied for each RSB1(/M) base unit on the included USB flash drive. Please see the chapter spectrum control for instructions on loading the calibration data into the software.
  • Page 46 For this calculation Thorlabs provides a .XLS worksheet supplied with the kit on the USB stick and instruction described below.
  • Page 47 Step-by-Step Procedure 1. Set up the RSB1(/M) Raman bundle with a Thorlabs front end as described above with the laser attached.
  • Page 48 Note Stray light entering the RSB1(/M) will falsify this calibration measurement and yield misleading data for all following measurements. 6. Acquire a dark image in the Settings ® Active Device window after switching to the “Dark Image”...
  • Page 49 Centroid Wavelength column) in the OSA Raman Software into column "Peak Position Measured" (letter D) of the EXCEL sheet, highlighted in green. The result might look like this: Page 42 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 50 In case the value deviates more, this might due to different reasons: a dusty Polystyrene chip, Polystyrene chip is not in the focal plane, or instability of the laser. If the problem per- sists, please contact Thorlabs. 11. Switch back to the OSA Raman Software and enter the determined wavelength value from cell "Laser Wavelength Solver [nm]"...

  • Page 51: Export Data

    · Save Trace / Save All in the Main Menu: Saves data in the file format .spf2x. · Export Trace: The software can also export spectral data in the following file formats: .csv, .txt, .jdx, .spc. Page 44 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 52: Experimental Measurements

    This chapter describes the procedure to start using the Raman Bundle and acquire a system test measurement. Calibration Steps: Ensure correct mounting of the RSB1(/M) system with a front end which suits your experi- ment: reflective front end RSBR1(/M) , cuvette front end...

  • Page 53: Sample Measurement

    RSBC1(/M): The cuvette of the RSBC1(/M) front end needs to be filled with sufficient sample volume to allow laser light to pass through. Please refer to the dimensions of Page 46 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 54: Compatible Cuvettes For Rsbc1(/M)

    Raman Spectroscopy Bundle Chapter 5: Operating Instructions the CVH100 cuvette holder and the chapter "Compatible Cuvettes for RBSC1(/M) " for details. Place a light shield around the sample (lid for RBSC1x and shield cover for RBSR1x) to prevent stray light from entering the optical path. Turn on the laser.

  • Page 55: Operating Principle

    Therefore, Raman spectroscopy is a valuable source for material analysis, complementary to other types of vibrational spectroscopy. Energy Diagram of the Exciting Photons in Different Applications. Page 48 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 56: Analysis Of Raman Spectra

    Raman Spectroscopy Bundle Chapter 6: Operating Principle 6.1 Analysis of Raman Spectra Raman spectroscopy analyses the shift in energy due to inelastic scattering caused by the Ra- man effect. The wavelength of the inelastically scattered photon is related to the excitation wavelength through the following energy equation that describes the Raman scattering event: E = E - E where...

  • Page 57: Acquisition Of Raman Spectra

    Raman peaks may also vary depending on the excitation wavelength range. Proper wavenumber scaling, as done in the Thorlabs factory, is therefore important from the point of measurement calibration. The imaging spectrograph is calibrated through wavelength dispersion only.
  • Page 58 Thorlabs RSBx1(/M) Bundle Features: The core of the Thorlabs RSB1(/M) is an imaging spectrograph for recording spectra in the spectral range from 815 nm to 915 nm, which fit Raman shifts with wavenumbers ranging from 500 cm to 1800 cm when using a 785 nm excitation laser source.

  • Page 59: Coded-Aperture Raman Spectroscopy

    Chapter 6: Operating Principle 6.3 Coded-Aperture Raman Spectroscopy The Thorlabs Raman bundle is based on an imaging spectrometer that features a diffraction grating as the dispersive element. Traditional spectrographs of this design face a trade-off between the desired spectral resolution and the achievable light throughput. This is because the dispersive element produces spectrally separable images of the same slit image.
  • Page 60 Chapter 6: Operating Principle ment . In case of the RSB1(/M) Thorlabs Raman Base Unit this is a Hadamard mask of order 64 with a matrix element size of 36 mm x 36 mm. The coded aperture increases the light throughput without damaging the system resolution. The sepctral resolution is defined by the size of the single mask element.

  • Page 61: Parameters Affecting Spectra

    To compensate for this and allow compar- ison of acquired data to databases, each unit of the RSB1(/M) is calibrated over the whole measurement range using NIST standards. For RSM1(/M), this calibration is done at the factory.
  • Page 62 This background level is even modulated by amplitude correction. Please note that for the Thorlabs Raman bundle this subtraction will not entirely eliminate the flat background level, because residuals of Poisson noise are still accountable after averaging of hundreds of thousands of pixels as it is done with the large coded-aperture.
  • Page 63 Ra- man scattering response. Therefore, a Coded-Aperture Raman Spectrosocpy Systems, like the Thorlabs Raman Bundle RSB1(/M) are more powerful in the analysis of complex mixtures com- pared to convetnional slit systems.
  • Page 64 Raman Spectroscopy Bundle Chapter 6: Operating Principle Note Because the sampling volume of Coded-Aperture Raman Spectrosocpy Systems is rel- atively large, the spatial resolution of the Raman analysis in these systems is limited when com- pared to slit Raman Spectroscopy systems. Excitation Power Because the number of scattered Raman photons per time is linearly proportional to the intens- ity of the excitation source, it is critical to use enough laser power.

  • Page 65: Maintenance And Service

    Thorlabs for return instructions. Please keep the RSB1(/M) away from dust, especially when the dust cover on the input aper- ture is removed. Debris may collect on the coded aperture of the RSB1(/M). Care must be taken when cleaning a sensor faceplate to avoid damage to the sensor. Please use every pre- caution to avoid contact with the coded aperture.

  • Page 66: Troubleshooting

    · OSA Raman Software does not Recognize the RSB1(/M) Base Unit Ø The software version might not support the RSB1(/M) Base Unit. Please be ensure to use the OSA Raman Software provided on the USB stick included with the RSB1(/M) or...
  • Page 67 Ø This is because of the constant presence of intrinsic noise. · Thorlabs OSA Raman Software cannot Find any Instrument Ø If the instrument was connected after the Thorlabs OSA Raman Software was started, it will not automatically detect the instrument. Press the button “Scan USB” found under “Sweep”...
  • Page 68 Raman Spectroscopy Bundle Chapter 7: Maintenance and Service Raman Bundle in the "Device Manager" List · After Starting the Measurement the Screen does not Update. Ø Start by checking your settings Ø Make sure at least one trace is set to “Write” or “Average”; see section Trace Update Op- tions Ø...

  • Page 69: Appendix

    Please find the technical data for RSB1/M and RSB1 Base Unit RSBR1/M and RSBR1 re- flective front end RSBC1/M and RSBC1 cuvette front end RSBA1 adapter for cage mounting on RSB1(/M) Base Unit below. Page 62 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 70: Rsb1/M And Rsb1 Technical Data

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.1.1 RSB1/M and RSB1 Technical Data All technical data are valid at 23 ± 5 °C and 45 ± 15% rel. humidity (non-condensing). RSB1/M RSB1 Item # Optical Specifications Wavelength Detection Range 815 nm to 915 nm Spectral Resolution (FWHM) (Wavelength) <0.65 nm...

  • Page 71: Rsb1/M And Rsb1 Dimensions

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.1.1.1 RSB1/M and RSB1 Dimensions RSB1/M Page 64 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 72 Raman Spectroscopy Bundle Chapter 8: Appendix RSB1 Page 65 MTN022733-D02...

  • Page 73: Rsbr1/M And Rsbr1 Technical Data

    600 mW MM-Fiber with 105 mm Core Focal Volume ~ 10 mm General Specifications Interface to RSB1(/M) Base Unit SM1 (1.035"-40) Internal Thread 75.0 mm x 99.1 mm x 114.6 3.00'' x 3.89'' x 4.36'' Dimensions (L x W x H) mm (2.95'' x 3.90'' x 4.51'')

  • Page 74: Rsbr1/M And Rsbr1 Dimensions

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.1.2.1 RSBR1/M and RSBR1 Dimensions RSBR1/M Page 67 MTN022733-D02...
  • Page 75 Raman Spectroscopy Bundle Chapter 8: Appendix RSBR1 Page 68 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 76: Rsbc1/M And Rsbc1 Technical Data

    Dimensions: 12.5 mm x 12.5 mm x 45 mm (0.49" x 0.49" x 1.77") Compatible Cuvette Type Optical Axis Height Above Cuvette Base: 8.5 mm (0.33") Four Polished Sides Interface to RSB1(/M) Base Unit SM1 (1.035"-40) Internal Thread Dimensions with post in Min position 69.3 mm x 115.2 mm x 133.4 mm 2.66'' x 4.54'' x 5.19''...

  • Page 77: Rsbc1/M And Rsbc1 Dimensions

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.1.3.1 RSBC1/M and RSBC1 Dimensions RSBC1/M Page 70 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 78 Raman Spectroscopy Bundle Chapter 8: Appendix RSBC1 Page 71 MTN022733-D02...

  • Page 79: Rsba1 Technical Data

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.1.4 RSBA1 Technical Data 8.1.4.1 RSBA1 Dimensions Page 72 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 80: Thorlabs Raman Bundle Technical Data

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.1.5 Thorlabs Raman Bundle Technical Data This section contains specifications for the Raman bundle consisting of the RSB1(/M) Base Unit and a Thorlabs Raman front end, either RSBR1(/M) or RSBC1(/M): Specifications Raman Spectroscopy Bundle Exposure Time: Technical Limitations 0.036 ms (Min) to 22795 s (Max) in 0.022 ms Increments...

  • Page 81: Technical Data Explained

    Usually, peaks can be identified at an SNR exceeding two to three, while for quantification, a higher SNR may be necessary. The SNR specified for RSB1(/M) in the technical data is based on the Raman Spectrum of the calibration polystyrene sample (RBC). The spectrum is acquired with the RSB1(/M) Base Unit and RSBC1(/M) front end at an excitation power of approximately 250 mW and with the dark image subtracted.

  • Page 82: Spectral Accuracy And Spectral Resolution

    The SNR characterization includes exposure times up to 20s, which is the maximum exposure time of the RSB1(/M) built-in camera. Note that averaging multiple exposures can increase the SNR, but once it is above 50-100 s total exposure time, it no longer follows the same trend and does not increase anymore.
  • Page 83 RSB1(/M)’s spectral range. The red arrow in the inset marks the selected spectral line. The right figure below is a magnified view of the figure to the left. The spectral accuracy...
  • Page 84 RSB1(/M), with an average value of 0.54 nm. For the purpose of the Thorlabs Raman bundle, Thorlabs specifies spectral resolution to be better than 0.65 nm, again deduced from the 3σ...

  • Page 85: Amplitude Correction

    8.1.7.3 Amplitude Correction Each spectrometer contains components (grating, AR coatings, detector etc.) with wavelength- dependent efficiency. In the RSB1(/M) Base Unit, the most important components to consider are grating and camera. The corresponding efficiency curves shown in Typical Dark Spectrum...
  • Page 86 ) which leads to an (unavoidable) decrease in SNR over the spectral range of the RSB1(/M). For the Thorlabs Raman bundle, NIST amplitude correction is enabled by default. To disable amplitude correction, place a check mark next to ‘Do not apply amplitude correction’ in vanced Mode Settings ->...

  • Page 87: List Of Acronyms

    Aspirin: 36 %, Titanium(VI) Oxide: 14 %, Caffeine: 11 % The red line shows the spectrum measured by the RSB1(/M) and the blue line shows a linear combination of four database spectra. All the information necessary for automatic amplitude correction is included within the calibra- tion data supplied with each Raman bundle on the USB stick.

  • Page 88: References

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.3 References 1) U.S. patents ## 7092101, 7301625, 7505130, EU patent # EP1839020, Japan patent # JP5220420 2) Static two-dimensional aperture coding for multimodal, multiplex spectroscopy” by Michael E. Gehm, Scott T. McCain, Nikos P. Pitsianis, David J. Brady, Prasant Potuluri, and Michael E. Sullivan –...

  • Page 89: Application Note Polarization Dependent Raman Spectroscopy

    < 0.75. In contrast, a vi- brational mode of r ³ 0.75 is not completely symmetric and is called a depolarized band. This application note describes how to use the Thorlabs Raman bundle to investigate polariza- tion dependent Raman scattering.
  • Page 90 This leads to increased background and noise levels within the spectral range of the base unit. Therefore, in the 180° transmission setup, it is recommended to use additional excit- ation wavelength filtering in the Raman scattered light path, e.g. using Thorlabs item # FEL0800 or # FELH0800.

  • Page 91: Optimizing Raman Signal Via Collimator Rotation

    Chapter 8: Appendix 8.4.1 Optimizing Raman Signal via Collimator Rotation The starting point of this configuration is the Thorlabs Raman Base Unit RSB1(/M) with front end RSBC1(/M) as configured at the factory and set up as described in the manual , and a laser source fed to the FC/PC port of the RSBC1(/M) via a multimode fiber.

  • Page 92: Including Polarization Optics In The Excitation Path

    This allows to the determination of depolarization ratios by adding a polarizer and a half- wave plate to the excitation path. The following chapter describes the steps to modify the Ra- man System for polarized Raman measurements, starting from a running Raman RSB1(/M) Base Unit with the RSBC1(/M) front end.
  • Page 93 ADAFC1 mating sleeve within the driver and an armored fiber (e.g. MR16L01) for connecting the Thorlabs RSBx1 Raman Spectroscopy bundle and laser. However, in these measurements we wanted to preserve as much of the laser’s polarization and thus skipped the armored fiber.

  • Page 94: Including Polarization Optics In The Excitation And Raman Scattering Path

    Follow the safety procedures, use safety gear and get in touch with your laser safety officer. 2. Unscrew the connecting ring between the baffle assembly and RSBC1(/M) main body 3. Unscrew the baffle assembly from the RSB1(/M) Base Unit at the SM1 thread 4.

  • Page 95: References

    McCamant, D. W., Kukura, P., & Mathies, R. A. (2003). Femtosecond Broadband Stimulated Raman: A New Approach for High-Performance Vibrational Spectroscopy. Applied Spectroscopy, 1317- 1323. doi:10.1366/000370203322554455 Tuschel, D. (2014). Practical Group Theory and Raman Spectroscopy, Part II: Application of Polariza- tion. Spectroscopy. Page 88 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 96: Osa Raman Software: General Instructions

    The below chapter gives general instruction to the OSA Raman Software on the USB stick in- cluded with the RSB1(/M). 8.5.1 Interface Overview When the Thorlabs OSA Raman Software is first started, the main window shown below is composed of five areas: · The Main menu, containing the command buttons ·...

  • Page 97: Customizing The Display

    . A locked axis cannot be dragged or zoomed using the mouse or keyboard in order to prevent an accidental change of the data display range. Axis Options Dialog Opened for the Horizontal Axis Page 90 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 98: Zooming And Scrolling The Vertical Axis

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.5.2.2 Zooming and Scrolling the Vertical Axis The primary vertical axis can be zoomed and scrolled by using the slide bar displayed to the left of the vertical axis. In the same way, the secondary vertical axis can be zoomed and scrolled using the slide bar displayed to the right on the secondary vertical axis (this axis is only visible when a trace is plotted on it –...

  • Page 99: Displaying The Secondary Vertical Axis

    8.5.2.5 Displaying the Secondary Vertical Axis In the Thorlabs OSA Raman Software the traces can be shown on either the primary axis with its labels and units displayed to the left of the graph, or on the secondary axis with its labels and units displayed to the right of the graph.

  • Page 100: Hiding The Menus And Full Screen Mode

    Displaying Data on Both the Primary and Secondary Vertical Axes 8.5.2.6 Hiding the Menus and Full Screen Mode There are two buttons located in the top right corner of the main menu, close to the Thorlabs logo as shown below ·...

  • Page 101: Displaying A Legend In The Data Display Area

    (e.g., ‘\mu’ will produce µ). The name of the trace and the comment of the trace can also be modified through the Trace info dialog, as described in section “Viewing properties of a trace ”. Page 94 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 102: Dark Room Mode

    Raman Spectroscopy Bundle Chapter 8: Appendix Show a Legend in the Data Display 8.5.2.9 Dark Room Mode If the screen is too bright, the graph background and grid color can be easily changed by click- ing the “Dark Room Mode” button under the “Display” menu. The background color will turn black and the grid color will turn white.

  • Page 103: Acquiring Data

    8.5.3.1 Finding the Spectrometer When the software starts it will automatically detect and read the properties of all Thorlabs spectrometers connected to the computer through USB. If the program was started before the instrument was connected or powered-on, the software will not be able to find the instrument.

  • Page 104: Acquiring A Single Spectrum

    Raman Spectroscopy Bundle Chapter 8: Appendix Devices Dialog Listing All Currently Connected Devices To acquire data from several connected devices simultaneously, start by setting up which trace should receive data from which device. This is done in the trace menu, which is opened by clicking the trace icon .

  • Page 105: Repeated Measurements

    In the Thorlabs OSA Raman Software, spectra are averaged as a rolling average, meaning that the screen will be updated for each new spectrum that is collected and the average of all the measured spectra will be displayed on the screen.

  • Page 106: Moving Markers

    Raman Spectroscopy Bundle Chapter 8: Appendix The movable markers can be used to inspect the value of the data at different positions, change the displayed area of the graph (see sections "Zooming and Scrolling the Horizontal Axis " and "Zooming and Scrolling the Vertical Axis "), or place fixed markers.

  • Page 107: Fixed Markers

    Movable Markers 8.5.5 Fixed Markers The Thorlabs OSA Raman Software can handle up to 2048 fixed markers. A fixed marker has a fixed horizontal position and is connected to a single trace (not necessarily the active trace) and will track the value of its trace at the given position. Fixed markers are identified by a number.

  • Page 108: Automatically Mark Peaks

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.5.5.2 Automatically Mark Peaks Pressing the button “Mark Peaks” will add one fixed marker to each peak in the currently active trace that has previously been automatically detected with the Peak track analysis tool (see section "Peak Track").

  • Page 109: Working With Data In Traces

    8.5.6 Working with Data in Traces The collected spectra are stored in what are called “traces.” The Thorlabs OSA Raman Soft- ware can handle up to 26 traces labeled from A to Z for spectra. The controls for the traces are found in the area between the data display area and the main menu.
  • Page 110 Raman Spectroscopy Bundle Chapter 8: Appendix the data. For devices with multiple detectors, it is also possible to select to view data from one of the detectors or a stitched combination thereof. · Fix: Traces with this option will not be updated at the next acquisition. ·...

  • Page 111: User-Defined Update Options

    “Math” under the “Math” main menu, see section “Math operations ”. Calculation of User-Defined Function From Measured Spectra in a Separate Trace and Scale Page 104 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 112: Viewing Properties Of A Trace

    Raman Spectroscopy Bundle Chapter 8: Appendix 8.5.6.4 Viewing Properties of a Trace To see the properties of a trace, click on the icon or the label of the trace that you want to in- spect, and select the menu item “View Info.” This will open a window displaying basic informa- tion of the trace, such as the serial number of the OSA Raman Software, date and time of the acquisition, and the settings that were used during the acquisition.

  • Page 113: Reordering Traces

    If the Trace was result of calculation, the related formula will be up- dated automatically. 8.5.6.6 Math Operations Thorlabs OSA Raman Software provides a range of pre-configured tools for post-processing of the spectra stored in traces, found under main menu "Math"...
  • Page 114 Raman Spectroscopy Bundle Chapter 8: Appendix The expression can also contain mathematical functions; available functions are listed and in- serted by clicking on the button labelled “Fx.” The calculation will be performed when the “Calculate” button is clicked or by pressing “Enter” on the keyboard.
  • Page 115 Figure 26 Smooth Operation Dialog Page 108 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 116 Raman Spectroscopy Bundle Chapter 8: Appendix Curve fit: Fits a mathematical curve to a trace or a region thereof. The Curve Fit dialog box will open, in which the fit settings and intput/output traces can be specified. Available math functions are: Gaussian, Lorentzian, and polynomial, see section "Functional traces ".
  • Page 117 If the original traces are not overlapping, then stitched values between the defined ranges will be set to zero. Page 110 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 118: Functional Traces (Traces Defined By A Function)

    ”. 8.5.7 Saving and Loading Data The Thorlabs OSA Raman Software can read and write the following spectral file formats: · Thorlabs OSA Raman Software Spectrum file format (.spf2x): Native file format for the general Thorlabs OSA Software. Please note, that only the Raman Edition of the OSA Software is compatible with Raman measurements.

  • Page 119: Saving Spectral Data

    Software Spectrum File format .spf2x or as raw data in ASCII file format. "File" Main Menu It is also possible to export the current data display into an image, using button “Save Image ”. Page 112 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 120: Loading Spectral Data

    8.5.8 Analyzing data The Thorlabs OSA Raman Software contains a number of analysis tools, each of which ana- lyzes one aspect of the data from the spectrometer. The result of each analysis is displayed in a panel below the main data display area, or in a separate floating window.

  • Page 121: Floating Windows

    “Display Analysis Region in graph” in the analysis settings window, which is opened by clicking on the small cogwheel icon in the upper left corner of the analysis window. The ana- Page 114 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 122 Raman Spectroscopy Bundle Chapter 8: Appendix lysis settings window also allows the fill color of the analysis region to be changed and the fill to be disabled entirely. By pressing the “Auto” button in the analysis window, the range and/or threshold will be set to values that are automatically determined from the displayed range of the main data display area.

  • Page 123: Peak Track

    Settings dialog box (described in section Peak Track Set- tings ) or directly in the tool options panel found to the left of the data table in the Peak track analysis window. Page 116 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 124 Raman Spectroscopy Bundle Chapter 8: Appendix For each peak that is found in the currently Active trace, one line is displayed in the table shown in the Peak track analysis window (by default found below the main data display area). The data reported for each peak is as follows: ·...

  • Page 125: Valley Track

    When the Valley track tool is active, the Active spectrum trace is checked for valleys upon the collection of a new spectrum from the instrument. By default, the valleys are sorted in order of increasing x-axis location. Right-clicking in the data table brings up a contextual menu from Page 118 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 126: Statistics Analysis

    Raman Spectroscopy Bundle Chapter 8: Appendix which it is possible to save the data in the data table to file, or copy the data to the clipboard for analysis in another software. If no valleys are found by the Valley track tool, check the Threshold and Min valley depth set- tings to make sure that the expected valleys in the spectrum are deeper than these constraints.
  • Page 127 “No” otherwise. · Contains Inf: Equal to true if the trace contains a (positive or negative) infinite value in the analyzed range, and equal to “No” otherwise. Page 120 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 128: Curve Fit

    Raman Spectroscopy Bundle Chapter 8: Appendix Statistics Analysis Window 8.5.8.6 Curve Fit The Curve fit tool allows a mathematical function to be fit to an active trace over user-specified x-axis and y-axis intervals. When the Curve fit tool is enabled, a curve fit analysis window is docked below the main data display area.
  • Page 129 When the fit is finished, the fit parameters as well as the values and standard errors thereof are displayed in a table in the Curve fit tool window. The fitted curve is also overlaid on the data in the main data display area for visual inspection. Page 122 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 130 Raman Spectroscopy Bundle Chapter 8: Appendix Curve Fit Tool Window The middle table shows the properties of the fit itself. The following parameters are displayed: · RSS (residual sum of squares): This is the sum of the squared differences between the fitted function and the trace, taken over all data points included in the fit.

  • Page 131: Baseline Correction

    (polynomial order + · Polynomial above: The baseline is determined in the same fashion as the Polynomial be- low method, but points lying above the fitted polynomial are selected instead. Page 124 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 132: Keyboard Shortcuts

    Raman Spectroscopy Bundle Chapter 8: Appendix The input trace, the calculated baseline, and the final corrected trace will be displayed in the Baseline correction dialog box. When you are satisfied, use the Output combo box to select the trace to which you wish to export the corrected trace and optionally the baseline profile, and click “Ok.”...

  • Page 133: General Osa Raman Settings

    If the check box “Activate persistence” is checked, the display will also display the last few spectra acquired in a more faded nuance. Note Enabling persistence might severely slow down the update rate of the screen. Display Settings Page Page 126 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...
  • Page 134 Raman Spectroscopy Bundle Chapter 8: Appendix 8.5.10.1.2 Peak Track Settings The automatic Peak track routine uses a number of parameters for finding peaks in the spectra. These options are the same as can be specified in the options panel for the Peak track analysis tool (see section Peak Track Peak Track Settings Page...

  • Page 135: Saving And Loading Settings

    Reset action does not destroy the instrument initialization and Calibration data. 8.5.10.2 Saving and Loading Settings When the Thorlabs OSA Raman Software closes, it will store the currently used settings to file and restore them during the next startup. These data are located in the User Profile’ folder “Ad- dData”:...
  • Page 136 Raman Spectroscopy Bundle Chapter 8: Appendix Save Acquisition Settings Dialog; Storing Settings to File for Later Use Saved settings can be restored in three ways: 1. Clicking on the small arrow next to the button “Load settings”, found under the “Sweep” menu in the main window, brings up a drop down list of the names of previously saved setups.
  • Page 137 Raman Spectroscopy Bundle Chapter 8: Appendix Figure 48:P Load Acquisition Settings Dialog, Allowing for Inspection of Settings Prior to Loading Page 130 Rev: 1.0, 17-Sep-2021 © 2021 Thorlabs...

  • Page 138: Safety

    If necessary, ask for replacement packaging. Refer servicing to qualified personnel! Changes to this device cannot be made nor may components not supplied by Thorlabs be used without written consent from Thorlabs. Users that change or modify the product described in this manual in a way not expressly ap- proved by Thorlabs (party responsible for compliance) could void the user’s authority to operate...
  • Page 139 Thorlabs warrants material and production of the Raman Spectroscopy Bundle for a period of 24 months starting with the date of shipment in accordance with and subject to the terms and conditions set forth in Thorlabs’ General Terms and Conditions of Sale which can be found at: General Terms and Conditions: https://www.thorlabs.com/Images/PDF/LG-PO-001_Thorlabs_terms_and_%20agreements.pdf...
  • Page 140 EC, and are not dissembled or contaminated. Contact Thorlabs for more informa- tion. Waste treatment is your own responsibility. “End of life” units must be returned to Thorlabs or handed to a company specializing in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.
  • Page 141 www.thorlabs.com...

This manual is also suitable for:

Rsb1/mRsbr1Rsbr1/mRsbc1Rsbc1/mRsba1

Table of Contents