THORLABS GANYMEDE II HR Operating Manual

THORLABS GANYMEDE II HR Operating Manual

Spectral domain oct system
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GANYMEDE II HR
Spectral Domain OCT System
Operating Manual

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Summary of Contents for THORLABS GANYMEDE II HR

  • Page 1 GANYMEDE II HR Spectral Domain OCT System Operating Manual...
  • Page 2: Table Of Contents

    Saturation and Non-Linearity ....................... 40 5.2. Wrong Reference Intensity Setting ....................... 41 5.3. Auto-Interference ........................... 42 5.4. Multiple Scattering ..........................43 5.5. Flipped Image ............................44 5.6. Shadowing ............................... 45 5.7. Image Distortion by Refractive Media ....................46 GANYMEDE-II-HR Page 2 www.thorlabs.com...
  • Page 3 Part 9. Specifications ..........................55 Part 10. Mechanical Drawings ....................... 56 Part 11. Regulatory ..........................58 11.1. Waste Treatment is Your Own Responsibility ..................58 11.2. Ecological Background .......................... 58 Part 12. Thorlabs Worldwide Contacts ....................59 GANYMEDE-II-HR Page 3 www.thorlabs.com...
  • Page 4 B-scan of a semi-transparent molded Plastic Cap ..............39 Figure 43 B-scan of a laminated IR Card (not included) ..............39 Figure 44 B-scan of a Section of a Grape .................... 39 Figure 45 High Surface Reflection Causing Saturation and Nonlinear Response ....... 40 GANYMEDE-II-HR Page 4 www.thorlabs.com...
  • Page 5 Fuse Cover on Base Unit Rear Panel ................... 52 Figure 61 base unit dimensions ......................56 Figure 62 Imaging probe dimensions ....................56 Figure 63 Dimension of the rotation stage underneath the sample plate ..........57 GANYMEDE-II-HR Page 5 www.thorlabs.com...
  • Page 6: Part 1. Introduction

    Opening the device will void your warranty. Any modification or servicing of this system by unqualified personnel renders Thorlabs free of any liability. This device can only be returned when packed into the complete original packaging, including all foam packing inserts.
  • Page 7: Safety

    Make sure that the line voltage rating agrees with your local supply and that the appropriate fuses are installed. Fuses should only be changed by qualified service personnel. Contact Thorlabs for assistance. Do not operate without cover installed. Refer servicing to qualified personnel.
  • Page 8: Care And Maintenance

    If this occurs, the system will need to be realigned by qualified personnel. If the system is dropped from a height greater than 15", Thorlabs will need to perform an electrical security check. Please contact Thorlabs Technical Support for more information.
  • Page 9: Fiber Cleaning Techniques Using The Fbc1

    Cleaning Fiber Bulkheads Remove the guide cap completely from the device, and insert the tip of the cleaner into the bulkhead connector. Push the case to start the cleaning process; a click indicates that the cleaning is complete. GANYMEDE-II-HR Page 9 www.thorlabs.com...
  • Page 10: Service

    Thorlabs provides. Any modification or maintenance by unqualified personnel will render the warranty null and void, leaving Thorlabs free of liability. Please contact Thorlabs Technical Support for questions on customization.
  • Page 11: Part 2. Description

    The lateral resolution, on the other hand, is affected by the chosen application optics. The SD-OCT system has four main parts: the base unit, a PC, an imaging probe and a probe stand (see Figure 4 above for a schematic diagram of the SD-OCT system). GANYMEDE-II-HR Page 11 www.thorlabs.com...
  • Page 12: Nomenclature In Oct Imaging

    A-scans, a 2-dimensional image is created. This is referred to as a B-scan. Here, the depth information is typically displayed from top to bottom, while the scan axis in Figure 2 is from left to right. Figure 6 shows a B-scan data set. Figure 6 B-scan Data Set GANYMEDE-II-HR Page 12 www.thorlabs.com...
  • Page 13: Figure 7 Rendered Volumetric Data Set

    When displaying a plane with both scan directions as axes, an en-face image is created. Here, the viewing plane is parallel to the image plane of the color camera inside the imaging probe. This plane is referred to as C-scan. Figure 8 En-Face View or C-scan GANYMEDE-II-HR Page 13 www.thorlabs.com...
  • Page 14: System Components

    Refer to the packing lists below to ensure that the system is complete. If any item is missing, contact Thorlabs for assistance. Do not use your own spare parts. Please use the appropriate tools when assembling a Spectral Domain SD-OCT system and handle all components of the system with care.
  • Page 15 1/4"-20 Cap Screw #8-32 Cap Screw 3/16" Hex Key 9/64" Hex Key Note: Before operating the system, check to see if any parts have been damaged during transportation. If so, please contact Thorlabs for further assistance. GANYMEDE-II-HR Page 15 www.thorlabs.com...
  • Page 16: Base Unit

    As such, near-IR broadband sources are a perfect compromise between sufficient transparency and a significantly reduced scattering coefficient. GANYMEDE-II-HR Page 16 www.thorlabs.com...
  • Page 17: Imaging Probe

    2.2.3. Imaging Probe Thorlabsʼ SD-OCT systems use a common path OCT setup in which the interferometer is located within the imaging probe (see Figure 10). Integration of the interferometer eliminates the problems associated with chromatic and polarization mode dispersion that are introduced by differences between individual fibers in the sample and reference arms.
  • Page 18: Figure 12 Optical Layout Of Imaging Probe

    The intensity of the back-reflected light can be adjusted using the reference intensity adjustment knob which will open or close a variable aperture inside the imaging probe. Figure 13 Reference Intensity Adjustment locked unlocked GANYMEDE-II-HR Page 18 www.thorlabs.com...
  • Page 19: Figure 14 Reference Length Adjustment

    In order to adjust the reference length adjustment knob, pull the knob approximately 5 mm outwards (see Figure 11) until you feel the knob coming to a rest . A clockwise rotation will increase the reference length. GANYMEDE-II-HR Page 19 www.thorlabs.com...
  • Page 20: Probe Stand

    Probe Stand The Thorlabs SD-OCT system is shipped with a probe stand. To set up the probe stand, please consult the instructions that you find in the probe stand box. The probe stand is equipped with a dove tail slide for holding the imaging probe.
  • Page 21: Figure 16 Oct Stand Coarse Adjustment And Cable Clip(Rear View)

    Fine focus adjustment can be done using the adjustment knobs depicted in Figure 17. Coarse Focus Coarse Focus Adjuster Adjuster Fine Focus Fine Focus Adjuster Adjuster Dove Tail Slide Lock Ring Figure 17 OCT Stand Adjusters and Dove Tail Slide GANYMEDE-II-HR Page 21 www.thorlabs.com...
  • Page 22: Figure 18 Oct Stand Sample Station

    The sample plate of the sample station can be removed using a counter-clockwise rotation while the rotation stage underneath is held in place. The bores underneath the rotation stage allow for the mounting of individual sample holders. A drawing of the rotation stage bores is shown in Figure 63. GANYMEDE-II-HR Page 22 www.thorlabs.com...
  • Page 23: Graphical User Interface

    Hardware control through the SDK ranges from low level functions such as setting the galvo scanners, to very powerful functions like initiating full 3D measurements. Using the SDK, the programmer can: • Define either a standard probe provided by Thorlabs or create a software representation of a custom-built probe. •...
  • Page 24: Warning Text

    LASER KLASSE 1M CLASS 1M LASER PRODUCT KLASSIFIZIERT NACH DIN EN 60825-1:2008 CLASSIFIED ACCORDING TO DIN EN 60825-1:2008 Figure 19 Laser Emission Warning Labels Figure 20 Base Unit Warning Labels Figure 21 Imaging Probe Warning Label GANYMEDE-II-HR Page 24 www.thorlabs.com...
  • Page 25: Part 3. Installation

     USB Data Port to Connect PC (USB 2.0 Type B Interface)  Fiber Plug (FC/APC)  Probe Connection Port (LEMO, 19 Pin)  Auxiliary Connection Port (LEMO, 14 Pin) – not used  CameraLink trigger (SMB)  CameraLink Base (MDR 26) GANYMEDE-II-HR Page 25 www.thorlabs.com...
  • Page 26: Figure 23 Interconnections Of The Imaging Probe

    Connections of the CameraLink Frame Grabber Card  CameraLink trigger connector (SMB)  CameraLink Port O connector(MDR 26) For the location of USB connectors, monitor connection, and AC plugs, please refer to the hardware manual shipped with the PC system. GANYMEDE-II-HR Page 26 www.thorlabs.com...
  • Page 27: System Installation

    3) Mount the probe in the probe stand by sliding the dove tail at the back of the Probe into the dove tail slide of the probe stand as shown in Figure 25 below. Dove Tail Slide Dove Tail Figure 25 Mounting the Imaging Probe into the Dove Tail Slide of the OCT Stand GANYMEDE-II-HR Page 27 www.thorlabs.com...
  • Page 28: Figure 26 Cameralink Frame Grabber Card Inside The Pc

    Secure the CameraLink plug with the screws attached to the connector. Trigger Connector CameraLink Connector Figure 27 Trigger and CameraLink Cable attached to the PC Attach the Trigger cable to the base unit as shown in Figure 28. Figure 28 Attaching Trigger Cable to Base Unit GANYMEDE-II-HR Page 28 www.thorlabs.com...
  • Page 29: Figure 29 Cameralink Cable Base Unit Attached To Base Unit

    6) Connect the power supply plug to the socket of the base unit and connect the other end to a wall plug (shown in Figure 30 below). Figure 30 Power Supply Plug Connected to the Base Unit GANYMEDE-II-HR Page 29 www.thorlabs.com...
  • Page 30: Figure 31 Plugging The Probe Connector Into The Imaging Probe

    (not shown)). Align the red dot of the plug to the alignment mark of the probe connection port (see Figure 31 below). red dot alignment mark Figure 31 Plugging the Probe Connector into the Imaging Probe Push the connector into the plug until a “click” sound is heard. GANYMEDE-II-HR Page 30 www.thorlabs.com...
  • Page 31: Figure 32 Installation Of The Fiber At The Probe Fiber Connector

    Also, the OCT focus position is affected by this. Secure the fiber connection by turning the lock cap clockwise. No force is needed for this operation. GANYMEDE-II-HR Page 31 www.thorlabs.com...
  • Page 32: Figure 33 Installing The Probe Connection At The Base Unit

    Push the connector into the plug until a “click” sound is heard. After installation, the connector should be locked. alignment mark red dot Figure 33 Installing the Probe Connection at the Base Unit GANYMEDE-II-HR Page 32 www.thorlabs.com...
  • Page 33: Figure 34 Installation Of The Fiber At The Base Unit Connector

    NOTE: If the key is not properly aligned with the slot, you will still be able to secure the fiber but there will be significant light loss produced by this incorrect connection. D) Secure the fiber connection by turning the lock cap clockwise. No force is needed for this operation. GANYMEDE-II-HR Page 33 www.thorlabs.com...
  • Page 34: Figure 35 Fiber Loop At The Base Unit

    Fiber Loop at the Base Unit 10) Pull the protection cap off the scan objective Do not rotate the protection cap, as this might loosen the fit of the illumination tube. protection cap Figure 36 Protection Cap Removal GANYMEDE-II-HR Page 34 www.thorlabs.com...
  • Page 35: Part 4. System Operation

    4.2. Basic Adjustments When receiving the SD-OCT system from Thorlabs, the reference length is adjusted so that OCT imaging in air is possible simply by adjusting the focus. Once the probe is significantly misadjusted, the following procedure provides basic adjustment guidelines.
  • Page 36: Adjusting The Reference Intensity

    Hit the auto-adjust button (see Software Manual) to adjust the dynamic range of your B-scan. When using the IR card for adjustment, your B-scan image should look as shown in Figure 39 on the next page. GANYMEDE-II-HR Page 36 www.thorlabs.com...
  • Page 37: Shutting Down The System

    Shutting Down the System The following steps should be followed when shutting down the system: 1) Save any important data. 2) Close the Thorlabs software. 3) Shut down the PC. 4) Turn the power switch on the base unit to “0”.
  • Page 38: Example Images

    Figure 40 B-scan of a Nailfold, imaged at 28kHz A-scan Rate Figure 41 B-scan of a Finger Tip, imaged at 28kHz A-scan rate GANYMEDE-II-HR Page 38 www.thorlabs.com...
  • Page 39: Figure 42 B-Scan Of A Semi-Transparent Molded Plastic Cap

    SD-OCT can also be used for non-biological material science applications, such as monitoring surface topography and layered structures. Figure 42 B-scan of a semi-transparent molded Plastic Cap Figure 43 B-scan of a laminated IR Card (not included) Biological Imaging Figure 44 B-scan of a Section of a Grape GANYMEDE-II-HR Page 39 www.thorlabs.com...
  • Page 40: Part 5. Imaging Artifacts

    Increasing A-scan rate (see software manual for details) Changing focus position Tilting the sample with respect to the A-scan axis Introduction of a wedge into the optical path (first reflex reflecting outside of NA) and immersion (see Figure 46) GANYMEDE-II-HR Page 40 www.thorlabs.com...
  • Page 41: Wrong Reference Intensity Setting

    When using a low reference intensity (see picture Figure 47A), the image becomes very noisy and auto- interference is strong compared to the intended signal; a frequent issue when measuring the thickness of reflecting films. High reference intensities (see picture Figure 47B) can cause saturation and loss of information. GANYMEDE-II-HR Page 41 www.thorlabs.com...
  • Page 42: Auto-Interference

    Auto-Interference with the Reference Arm of the Imaging Probe Deactivated When measuring the thickness of foils or similar reflective samples, auto-interference can be a desired feature. To avoid this effect, we suggest using index matching (see Figure 46) or tilting the sample. GANYMEDE-II-HR Page 42 www.thorlabs.com...
  • Page 43: Multiple Scattering

    In the OCT image acquired from this setup (see Figure 51 above), one can clearly see that the paper appears to be very thick. This apparent thickness is caused by the relatively long travel of photons that have scattered multiple times before finding their way back into the detecting aperture. GANYMEDE-II-HR Page 43 www.thorlabs.com...
  • Page 44: Flipped Image

    Correct Reference Length Setting for Imaging of an IR Card Volume Surface Figure 53 Incorrect Reference Length Setting showing a Flipped Image Figure 52 and Figure 53 show the scans of an IR viewing card when using correct and incorrect reference length settings. GANYMEDE-II-HR Page 44 www.thorlabs.com...
  • Page 45: Shadowing

    Reflections, strong scattering, and absorption can all lead to shadows in the depth distribution of the acquired data. Figure 54 Rendered Volume of a Screw on top of an IR Viewing Card displaying the Shadowing Effect GANYMEDE-II-HR Page 45 www.thorlabs.com...
  • Page 46: Image Distortion By Refractive Media

    Transparent Plastic Scattering Plastic Figure 55 Schematic of a Setup to show Distortions from Refractive Media Height Shift Figure 56 Height Shift of OCT Imaging through Refractive Media GANYMEDE-II-HR Page 46 www.thorlabs.com...
  • Page 47: Group Refractive Index

    In OCT systems, the group refractive index defines the optical path lengths. The table below shows the phase refractive indices and group refractive indices n of common materials. In vacuum and air the values for n as well as for are 1 for all wavelengths. GANYMEDE-II-HR Page 47 www.thorlabs.com...
  • Page 48: Measurement Depth In Oct Systems

    The loss of imaging depth depends on the thickness and the group refractive indices of the materials displayed within the image. It is calculated using the equation below:    loss GANYMEDE-II-HR Page 48 www.thorlabs.com...
  • Page 49: Distortions In The Image

    When the physical structure becomes more complex the resulting OCT image becomes more difficult to interpret. Especially when the sample surface is not horizontal or curved, effects like shadowing, diffraction on interfaces and possible multiple measured structures may occur in addition to the changes in optical path length. GANYMEDE-II-HR Page 49 www.thorlabs.com...
  • Page 50: Figure 59 Complex Structure In Image

    In very complex structures, these effects become more and more difficult to handle – Just assume spherical or curved interfaces, bubbles, inhomogeneous materials, possible imaging aberrations in the sample probe etc. GANYMEDE-II-HR Page 50 www.thorlabs.com...
  • Page 51: Part 6. Troubleshooting

    OCT image Adjust reference intensity Reference intensity too high or too low knob Other reason Call Thorlabs Flipped Image Reference length set incorrectly Adjust reference length Please refer to Part 12 for Thorlabs contact information. GANYMEDE-II-HR Page 51 www.thorlabs.com...
  • Page 52: Changing The Input Fuses

    Use only IS 2A 250VAC Type T 5x20mm style fuses (IEC 60127-2/III, low breaking capacity, slow blow).  Slide the fuse cover closed. 100-240 VAC 50-60 Hz 45W MAX. FUSE 500mA/250V TYPE T 250 Figure 60 Fuse Cover on Base Unit Rear Panel GANYMEDE-II-HR Page 52 www.thorlabs.com...
  • Page 53: Part 7. Certifications And Compliance

    Spectral Domain OCT System Part 7. Certifications and Compliance GANYMEDE-II-HR Page 53 www.thorlabs.com...
  • Page 54: Part 8. Warranty

    Part 8. Warranty 8.1. Lasers and Imaging Systems Thorlabs offers a one year warranty on all lasers and imaging systems, with the exceptions of laser diodes. 8.2. Non-Warranty Repairs Products returned for repair that are not covered under warranty will incur a standard repair charge in addition to all shipping expenses.
  • Page 55: Part 9. Specifications

    420 mm x 320 mm x 149 mm Dimensions of base unit (L x W x H) 60 mm x 85 mm x 187.8 mm Dimensions of Imaging Module (L x W x H) base unit has universal AC input GANYMEDE-II-HR Page 55 www.thorlabs.com...
  • Page 56: Part 10. Mechanical Drawings

    Spectral Domain OCT System Part 10. Mechanical Drawings Figure 61 Base Unit Dimensions Figure 62 Imaging Probe Dimensions GANYMEDE-II-HR Page 56 www.thorlabs.com...
  • Page 57: Figure 63 Dimension Of The Rotation Stage Underneath The Sample Plate

    Spectral Domain OCT System Figure 63 Dimension of the Rotation Stage Underneath the Sample plate GANYMEDE-II-HR Page 57 www.thorlabs.com...
  • Page 58: Part 11. Regulatory

    Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.
  • Page 59: Part 12. Thorlabs Worldwide Contacts

    Spectral Domain OCT System Part 12. Thorlabs Worldwide Contacts For technical support or sales inquiries, please visit us at www.thorlabs.com/contact for our most up-to- date contact information. USA, Canada, and South America UK and Ireland Thorlabs, Inc. Thorlabs Ltd. sales@thorlabs.com sales.uk@thorlabs.com...
  • Page 60 Thorlabs GmbH Hans-Boeckler-Str. 6 Dachau/Munich 85221 Germany Phone: +49 (0) 8131-5956-0 ♦ Fax: +49 (0) 8131-5956-99 www.thorlabs.com M0009-510-784 Rev.A...

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