GE SEMIPHOR TRANSPHOR Series Operating Instructions Manual page 58

7 Troubleshooting
Open circuit or no output current
for LARGE SEMIPHOR
TRANSPHOR UNIT and SEMIPHOR
TRANSPHOR UNIT
Problem
Open circuit or no output cur-
rent for LARGE SEMIPHOR
TRANSPHOR UNIT and
SEMIPHOR TRANSPHOR UNIT
Diffuse band patterns
Problem
Diffuse band patterns
58
Solution
Push circuit protection reset button located next
to the input leads. To prevent recurrence limit the
input voltage to 30 V and the current to 500 mA.
Early models: Blown fuse in protection circuit. Re-
place 1.6 A micro fuse.
Solution
Transfer immediately after electrophoretic sepa-
ration. If equilibrating before the transfer, shorten
or eliminate the equilibration time or move the gel
to the cold room during equilibration.
If transfer buffer contains methanol (≥ 10%),
equilibrate the gel in transfer buffer for 30 minutes
to allow it to shrink before assembling the stack.
Note:
Because methanol causes the gel to shrink slightly,
large molecules may migrate more slowly.
Make sure that the gel is held firmly against the
membrane and that it does not shift once contact
is made.
Buffer depletion can change the pH in the transfer
stack, and have a negative effect on the transfer.
On subsequent transfers, either shorten the
transfer time, reduce the current, or increase the
number of buffersoaked blotting papers in the
stack.
Check that the preferred binding surface of the
membrane (if any) contacts the gel.
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA