Electrotransfer With Large Semiphor Transphor Unit And Semiphor Transphor Unit - GE SEMIPHOR TRANSPHOR Series Operating Instructions Manual

5.2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT

5.2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR
UNIT and SEMIPHOR TRANSPHOR UNIT
Introduction
Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR
UNIT are performed at low current and low voltage, in approximately one hour.
This section describes how to run the electrotransfer using LARGE SEMIPHOR TRANSPHOR
UNIT and SEMIPHOR TRANSPHOR UNIT.
Recommended current settings
Use the graph to quickly find the maximum current setting for your gel size , or calculate
the gel area (cm
Gel size
(cm
14 × 24
14 × 16
8 × 10
8 × 7
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
2
) and multiply it by 0.8 mA.
500
)
2
400
300
200
100
0
0 100
45 65
200 300 400
Maximum
180 270
current (mA)
5 Operation
45