Electrotransfer With Pwr Large Semiphor Transphor Unit And Pwr Semiphor Transphor Unit - GE SEMIPHOR TRANSPHOR Series Operating Instructions Manual

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5.3 Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT

5.3
Electrotransfer with PWR LARGE SEMIPHOR
TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR
UNIT
Introduction
Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR
TRANSPHOR UNIT are performed at low current and low voltage, in approximately one
hour.
This section describes how to run the electrotransfer using PWR LARGE SEMIPHOR
TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT.
Recommended current settings
Use the graph to quickly find the maximum current setting for your gel size , or calculate
the gel area (cm
Gel size
(cm
14 × 24
14 × 16
8 × 10
8 × 7
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
2
) and multiply it by 0.8 mA.
500
)
2
400
300
200
100
0
0
100
45
65
200
300
400
Maximum
180
270
current (mA)
5 Operation
47

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