GE SEMIPHOR TRANSPHOR Series Operating Instructions Manual page 39

Step
6
7
8
9
10
Assemble a transfer stack with
a single gel
Follow the instructions below to assemble the transfer stack.
Note:
Step
1
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
Action
For each gel, cut at least 6 pieces of blotting paper the same size as the gel
or slightly smaller. Make sure to cut the blotting paper to the same size as
the gel.
Saturate the blotting paper with transfer buffer.
For each gel, cut 1 membrane the same size as the gel or slightly smaller.
Note:
Always wear gloves when handling membranes to avoid leaving fingerprints.
Note:
A larger membrane may touch an electrode panel, creating a pathway by
which current can bypass the gel.
Pre-wet nitrocellulose or nylon membranes with distilled water. Pre-wet
polyvinylidene difluoride (PVDF) or other hydrophobic membranes with
methanol.
Soak all membrane types in transfer buffer for 2 to 5 minutes.
Always wear gloves when handling membranes to avoid leaving fingerprints.
Action
One by one, center each sheet on the lower electrode. Refer to
of a transfer stack with a single gel, on page
NOTICE
Stack each layer with care, with edges parallel. As each
layer is added, remove all air pockets by rolling a clean
pipet from the center to the edges. Add a few drops of
buffer to trouble areas to help remove air pockets.
Note:
For best results, the transfer stack should be centered on the electrode panels.
5 Operation
5.1 Assemble transfer stack
Illustration
37.
39